Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 99 for “"Clonogenic"”.
-
Identification and Characterization of Distinct Populations of Clonogenic Bone Marrow Stromal Cells Capable of Transferring The Hematopoietic Microenvironment In Vivo and Supporting Lt-Hscs In Vitro
… in the mouse are complicated by the low yield of clonogenic progenitors (CFU-F). Given the perivascular location of MSC in BM, we developed an alternative methodology to isolate MSC from mBM. An intact ‘plug’ of bone marrow is expelled from bones and enzymatically disaggregated to yield a single …
-
Functional Consequences of Matrix Metalloproteinase-1 Over-Expression in Human Gliomas
… a collagen type-I invasion assay along with clonogenic and ATP viability assays. Data presented demonstrate that MMP-1 over-expressing cells were more invasive in both cell types and interestingly more clonogenic in on of the glioma cell lines, supporting a possible role for MMP-1 in glioma …
-
Effects of Concurrent Radiosensitization and Chemoradiotherapy for Brain Tumors
… chemoradiotherapy. We also have a focus on clonogenic assays for cell lines treated with this same broad range of quantum dots with (TMZ) for measurement of cell survival two- and three-weeks posttreatment. Using a standard laboratory cell irradiator (Faxitron), we irradiate glioblastoma …
-
Effects of Green Tea Extract (GTE) for Normal and Cancer Cell Death by Radiation
… 단독으로 처리하거나 녹차추출물 처리 후 방사선을 조사하고 MTT assay, clonogenic assay, ROS assay를 시행하였다. 방사선과 함께 0.025 및 0.05 mM의 녹차추출물을 사용하였을 때 방사선만을 조사받은 경우에 비해 정상 세포주인 IEC-6에서 방사선조사 후 세포생존율이 증가하였으며 방사선조사를 받지 않은 경우에도 같은 비율로 세포생존율의 증가가 있었다. 대장암 세포주인 HCT-116에서는 0.025 및 0.05 mM 녹차추출물 처리로 방사선에 의한 세포살상이 증가하였다. 0.05 mM보다 …
-
Dose-rate-effects in XRCC1 wild-type and mutant CHO cell lines using An ²⁴¹AM source
… cell lines. In particular, this study performed clonogenic survival and growth assays to determine the radiations/ effect on the cells proliferative capacity. It was hypothesized that the XRCC1 null mutants would show greater radiosensitivity during continuous low-dose-rate radiation since the …
-
DNA repair deficiencies as a biomarker for treatment response in acute myeloid leukaemia
… cell lines to be used as in vitro models. Clonogenic assays, flow cytometry and immunofluorescence was used to assess the repair efficiency of the in vitro models. A pooled CRISPR screen and single cell sequencing ascertained which mutations may be driving the DDRD phenotype in patients. …
-
The impact of folate deficiency on the base excision repair pathway: Analysis of enzyme coordination in response to dna damage and imbalanced repair
… of DNA base lesions and damage can result in clonogenic selection of initiated cells, driving carcinogenesis.</p>
-
Role of Actin and its regulating proteins in drug response
… in chapter 4, gene silencing and drug treated clonogenic assays were performed to elucidate the functional role of LIMK1 and LIMK2 in drug response. Silencing of LIMK1 and/or LIMK2 increased sensitivity of neuroblastoma cells to microtubule targeting drugs and DNA damaging agents, suggesting …
-
Regulation of cancer progression by hepatitis B virus X protein (HBx) and HBxAP/RSF1
… 3. Western blotting . 5 4. Luciferase assay 5 5. Clonogenic cell proliferation assays 5 6. Patients characteristics 6 7. Statistics 6 C. RESULTS 7 1. The protein levels of HBx are decreased by catalase and MnSOD 7 2. Catalase downregulates HBx protein levels at the translational level 7 3. …
-
Novel Drug Discovery for Glioblastoma Multiforme Using Brain Tumour Initiating Cells
… R333 dramatically decreased the viability and clonogenic potential of diverse BTIC lines. In vivo, systemic treatment with R348, the prodrug form of R333, was well tolerated and demonstrated favourable pharmacokinetic properties. R348 treatment was also found to lead to a significant increase …
-
THE EFFECT OF SILENCING THE WILMS' TUMOR 1 GENE ON THE RADIATION SENSITIVITY OF GLIOBLASTOMA CELLS
… was assessed using a luminescent ATP assay and clonogenic survival assay. We demonstrate that treatment with WT1 siRNA increased the radiation sensitivity in both cell lines. These findings suggest that WT1 functions to protect glioblastoma cells from radiation-induced death.
-
Understanding Ontogeny and Optimizing Strategies to Promote Hematopoietic Engraftment Following In Utero Transplantation
… hematopoietic, and, surprisingly, possess higher clonogenic potential compared with their fetal counterparts. Finally, using the ovine model of in utero transplantation, we demonstrated that manipulation/priming of the fetal HSC niches, by transplanting adult stromal/ endothelial cells prior to …
-
Development of Lineal Energy Spectrum-Based Biological Effects Models for Protons
… of protons began. Using previously gathered clonogenic cell survival response data of H460, H1437, U87, and AGO cell lines following proton irradiation, mathematical models describing the relative biological effectiveness of proton therapy as a function of lineal energy and linear energy …
-
Direct and indirect effects of alpha-particle irradiations of human prostate tumor cells
… of DU-145 cells were studied using the clonogenic assay, the comet assay and the micronucleus assay. A novel co-culture system was then designed to study the bystander effect transmitted through the cell culture medium. The micronucleus assay was used as endpoint for the bystander …
-
Characterization of Thy-1 expression on human hematopoietic cells
… the basis of Thy-1 expression, the majority of clonogenic hematopoietic progenitor cells were recovered in the Thy-1⁻CD34⁺ fraction, whereas the majority of cells giving rise to clonogenic progenitors after 5-8 weeks of culture on irradiated marrow adherent feeder layers (i.e., long-term culture …
-
Post-translational modifications of c-FLIP: opportunities for therapeutic intervention
… enhanced apoptosis and reduced long-term clonogenic survival. Furthermore, apoptosis arising from vorinostat and cisplatin co-treatment was FLIPs- and caspase-8-dependent.<br/><br/> TRAIL is an anti-cancer agent that activates the extrinsic apoptotic cascade through the death receptors. …
-
The effect of distinct BRCA1 splice forms on drug sensitivity in breast- and ovarian cancer cell lines
… olaparib. Drug sensitivity was studied by clonogenic assay, and treatment with olaparib demonstrated that cells with loss of BRCA1 expression are more sensitive to PARP inhibitors than cells expressing wildtype (WT) BRCA1. The clonogenic assay also revealed that cells with BRCA1 loss grew …
-
Physical and biological characterisation of clinically relevant combined MRI-radiation exposures with conventional and nanoparticle contrast agents
… contrast agents was determined primarily through clonogenic survival assays and DNA damage immunofluorescence assays, at both 225 kVp and 6 MV energies, with and without the presence of a 1.5 T static magnetic field. The contrast enhancement was determined for several different CT and MRI scanners …
-
Loperamide enhances bortezomib-mediated cell death in colon cancer cells
… cytometry for the analysis of DNA contents 13 M. Clonogenic cell survival assay 13 III. RESULTS 14 1. Synergistic cell death induced by loperamide plus bortezomib is neither apoptosis not autophagic cell death 14 2. Intracellular Ca2+ influx is involved in the combined treatment-induced …
-
Induced Cytotoxicity In Crebbp/Ep300Mut Head and Neck Squamous Cell Carcinoma
… lines HN31 and FADU were used in cell culture. Clonogenic survival assays were used to cell growth and death with the different agents (DOX, CDDP, XRT with or without A485) used in single and combination treatments. Flow cytometry was used to measure cell death and apoptosis. Cell Titer Glo was …
Page 1 of 5