Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 831 for “"Cloning"”.
-
MicroRNA cloning and bioinformatic analysis
Part I. Two gene-regulatory noncoding RNAs (ncRNAs), let-7 RNA and lin-4 RNA, were previously discovered in the C. elegans genome. The let-7 gene is conserved across a wide range of genomes, suggesting that these ncRNAs represent a wider class of gene-regulatory RNAs. Both lin-4 and let-7 RNAs are …
-
Human cloning : science, ethics, policy, society
… to our understanding of and relation with human cloning. Genetic science and technology at the end of the twentieth century has permitted successful cloning of mammals and other animals. Such advancement has raised key ethical issues regarding the prospect of cloning human beings. Evaluation of …
-
Facial Expression Cloning with Fuzzy Membership Functions
… and experimental results of a system to explore cloning of facial expressions between dissimilar face models, so new faces can be animated using the animations from existing faces. The system described in this thesis uses fuzzy membership functions and subtractive clustering to represent faces …
-
Gene cloning studies in two nocardioform bacteria
… wastes and are important medically. A gene cloning vector designated pLR591 was constructed from the broad host range, multicopy Streptomyces plasmid pIJ702 and the positive selection Escherichia coli plasmid pEcoR251. This plasmid has useful features for the construction of actinomycete …
-
Optimizing Conditions for Cloning cDNA from Chicken Brain
… techniques including cDNA synthesis and cloning were attempted first with the chicken brain. The initial attempts at making cDNA libraries were elusive; therefore, experiments were performed to identify problems and enhance the efficiency of specific steps. These steps included RNA …
-
Cloning and characterization of MET2 in Pichia pastoris
<p>The methylotrophic yeast, Pichia pastoris, has been used as a protein expression system to express over 500 heterologous proteins. P. pastoris provides many advantages over other organisms that have been utilized for this purpose. In this project, we developed a new host/selectable marker and …
-
The cloning and characterization of the TIK kinase.
… work carried out in this thesis describes the cloning and characterization of the TIK kinase, a novel member of the protein kinase family. The TIK kinase was isolated and cloned from murine pre-B cells on the basis of its immunoreactivity with antibodies to phosphotyrosine, and was therefore …
-
Cloning and Overexpression of Yeast Cystathionine γ-Lyase
Cystathionine γ-lyase (CGL) is a protein involved in the conversion of methionine to cysteine as part of the trans-sulfuration pathway. Inactivation of the enzyme, possibly by oxidation of reactive site residues, has been linked to cataractogenesis. This research isolated the cystathionine γ-lyase …
-
Cloning and Molecular Characterization of Novel Animal Papillomaviruses
… some cancers. This thesis describes the cloning and molecular characterization of five new animal papillomaviruses: an equine cutaneous papillomavirus (EqPV); a canine oral papillomavirus associated with vaccine-induced carcinomas (COPV); an avian papillomavirus (PePV); an oral …
-
Cloning of a putative human oncogenic virus, BK
Papova viruses are a group of non-enveloped icosahedral viruses which contain a double-stranded circular DNA genome in the supercoiled configuration. There are two subgroups, i.e., the papilloma and the polyoma viruses. The papilloma viruses are generally larger than the polyoma-viruses, having a …
-
Towards Cloning the Leaf Rust Resistance Gene Rph5
Leaf rust caused by Puccinia hordei is an important disease of barley (Hordeum vulgare) in many regions of the world. Yield losses up to 62% have been reported in susceptible cultivars. The Rph5 gene confers resistance to the most prevalent races (8 and 30) of barley leaf rust in the United States. …
-
Cloning and expression of xylanase variants in Pichia pastoris
Submitted in fulfilment for the requirement of a Degree of Master in Biotechnology, Durban University of Technology, Durban, South Africa, 2017.
-
Cloning and characterization of the Pichia Pastoris PMR1 gene
<p>Pichia pastoris, a popular protein expression system, is limited in its ability to secrete heterologous proteins. The PMR1 gene, the disruption of which is known to improve the secretion of prochymosin, human prourokinase, and human tissue plasminogen activator in Saccharomyces cerevisiae, was …
-
Tobacco Phospholipase D β1: Molecular Cloning and Biochemical Characterization
Transgenic tobacco plants were developed containing a partial PLD clone in antisense orientation. The PLD isoform targeted by the insertion was identified. A PLD clone was isolated from a cDNA library using the partial PLD as a probe: Nt10B1 shares 92% identity with PLDβ1 from tomato but lacks the …
-
Cloning, Purification, and Biochemical Characterization of Human Prolyl Endopeptidase
… and 3’ ends necessary for ligation independent cloning (LIC) into the expression vector. This was then expressed in BL21(DE3)/pTFs and purified on a nickel column. Future studies will include comparing inhibition between hPEP and spPEP using 16 PCR amplified fragments of varying length that …
-
Cloning and characterization of novel kinases from embryonic cells.
… to identify novel PYKs through expression cloning strategies have led to the identification of a novel family of protein kinases, referred to as dual specificity kinases (DSKs). In addition to their immunoreactivity with antiphosphotyrosine antibodies, DSK family members have the ability to …
Page 1 of 42