Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 21 for “"Clonal selection"”.
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Clonal selection and characterization of epigenetic variation in Pichia pastoris
Recombinant proteins produced by different host organisms have been broadly used as therapeutics. Considering the demand for large quantities of protein drugs, methods are needed to increase reactor titers in a timely and cost-effective manner. We used random chemical mutagenesis to modify a …
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Estudio del origen genético de la variedad de vid Garnacha Blanca, de su diversidad fenotípica y de los efectos moleculares asociados a la variación en el color de la uva
… to improve traditional varieties either by clonal selection, or for the generation of new derivative varieties, like berry color variants. Garnacha Blanca (GB) is a white-berried variety derived from Garnacha Tinta (GT). Both varieties display fruit set problems that can limit production. In …
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A Molecular Cytogenetic Profile of the Chronic Myeloproliferative Disorders
… whether application ofGF stimulants leads to clonal selection, cultured samples from 15 patients were analysed by Fluorescence In situ Hybridisation (FISH), which supported the theory that clonal selection remained unaltered in stimulated samples. In addition to this, immunophenotyping of …
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Immunologic Memory to Polysaccharide Antigens
… against previously encountered antigens. The clonal selection theory of acquired immunity attributes immunologic memory to the formation of an expanded compartment of quiescent antigen-specific memory B cells that are capable of rapid activation upon secondary antigen encounter. Whereas …
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Investigating the Effect of Antibody-Mediated Feedback on Ongoing Germinal Center Responses
… onto contemporaneous GCs to influence B cell clonal selection and affinity maturation in real-time remains poorly understood. Here we present a genetic mouse tool to interrogate the effect of antibody-mediated feedback on ongoing GC responses. We have designed an oligoclonal B cell transfer …
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The mutational landscape of normal urothelium
… their histologically normal appearance. These clonal expansions are commonly driven by mutations in known oncogenes and tumour suppressor genes, with the driver landscape in a given tissue resembling its associated cancer. Bladder cancer exhibits complex landscapes of clonal selection and …
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Studying the Clonal Origins of Drug Resistance in Human Breast Cancers
… giving a new depth to our understanding of clonal selection in cancer. In particular, high complexity barcodes are used to identify a pre-existing tamoxifen resistant population in the MCF7 cell line. This resistance phenotype is then linked to the induction of embryonic transcription factor …
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Hapten Binding by Lymphocyte Surface Immunoglobulin and the Subsequent Implications for Affinity Maturation
… be a continuous and necessary feature of B cell selection and differentiation. Fourth, naive lymphocytes from BALB/cV mice, selected according to smIg avidity on fluorescein affinity plates failed to demonstrate on anti-fluorescyl response upon adoptive transfer into irradiated recipients. In …
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Differentiation of Normal and Cystic Fibrosis Human Lung Epithelial Cells in a Decellularized and Reconstituted Mouse Lung
… derived human bronchial epithelial cells and clonal selection without the need for genetic manipulation; facilitating the study of lung diseases and optimization of organ reconstitution in tissue engineered models.
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Intestinal CD4+T Cell Responses to Food
… We also observed a strong bias towards clonal selection of mature intestinal CD4<sup>+</sup> T cells by dietary proteins, suggesting that food antigen-specific intestinal CD4<sup>+</sup> T cells are generated in the course of eating. Finally, we show that intestinal inflammation induced …
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Decoding Human T Cell Immunity With Single-Cell Multi-Omics
… comparison, and make the case for convergent clonal selection after infection through the discovery and characterisation of TCR motifs. Next, I link TCR motifs to antigen specificity using experimental T cell specificity databases and validate the findings with in-house data in the context of …
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A cell-based sensor of fluid shear stress for microfluidics
… sensor was created by stable transfection and clonal selection. Inducing the pathway with Phorbol-myristate-acetate resulted in fluorescence induction by both microscopy and flow cytometry, verifying the sensor functionality. The fluorescence activation was then characterized across PMA doses …
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Characterizing the Role of TLX1 in T-cell Acute Lymphoblastic Leukemia
… in T-ALL tumors suggests a complex process of clonal selection through which oncogenic TF-expressing pre-leukemic cells gradually transition along the path of oncogenic transformation3,4. While transgenic mouse studies have established the causative role of primary oncogenic TFs in driving …
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The natural history of clonal haematopoiesis
… throughout life, some of which can drive clonal expansion. Such expansions are frequent in the haematopoietic system of healthy individuals and have been termed clonal haematopoiesis (CH). While CH predisposes to myeloid neoplasia and other diseases, we have limited understanding of its …
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Isolation, Culture and Cryopreservaion of Neural Stem Cell
… neurosphere from single cell, we selected by clonal selection and cultured for 10 day. Single cell was formed neurosphere and expressed GFP or not. Neural stem cells (NSCs) derived from fetal and adult central nervous system has been expanded, differentiated, and fount to be multipotent and …
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Somatic variation, carcinogenesis and cancer risk in the lung
… and risk factors alter mutation rates and clonal expansions has only recently become possible. In this thesis, we explore how evolution shapes the landscape of somatic mutations and clonal populations within an individual. Specifically, we use the lung and lung cancer as a primary example, …
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An Online Adaptive Machine Learning Framework for Autonomous Fault Detection
… of this research is the development of a Clonal Selection Algorithm that optimizes the OSVM hyperparameters and the V-Detector algorithm parameters, resulting in a more effective fault detection solution. The integration of the AIS in the training process enables the generation of …
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An investigation towards a conjugate vaccine against Streptococcus pneumoniae serotype 19A
… the model Pn1. The cultivation process included clonal selection for the growth and isolation of a serotype 19A clone producing high levels of CPS and cultivation using disposable bag technology as an alternative to the traditional fermentor. The culture was inactivated at low temperatures using …
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Factors Influencing the Somatic Mutational Landscape of Ageing Squamous Epithelium
… sites reveals differences in UV signature and selection between sites. In aged oesophageal epithelium from UK donors, I observe an increase in mutations with an alcohol-associated signature with reported alcohol consumption. Furthermore, mutation burden increases with smoking, without a …
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Evolutionary biology of Fusarium oxysporum f.sp. cubense
… groups, and can be divided into a number of clonal lineages that roughly correspond with VCG groupings. In this thesis, we investigated the evolutionary relationships among VCGs using multi-gene sequencing and MAT genotyping. A PCR-RFLP fingerprint discriminating the Foc lineages and a PCR …
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