Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 18 of 18 for “"Clonal Analysis"”.
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Opposing Crosstalk between Rho1 and Cdc42 in Epithelial Morphogenesis
… allows for straightforward loss-of-function analysis, in vivo. To determine the role of Rho1 and Cdc42 in Drosophila epithelia, we used mosaic clonal analysis and targeted RNA interference expression to perform loss-of-function studies with Rho1 and Cdc42 during pupal eye and larval imaginal …
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Functional and computational analysis of RNA-binding proteins and their roles in cancer
… browser. Appendix C describes a method for clonal analysis of neural stem cell growth and differentiation in culture using live imaging and `microdot' plates, developed as part of the work presented in part one of the thesis.
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Genetic Recombination in Pneumococcal Transformation: The Role of Integrity and Heterogeneity of Deoxyribonucleate Molecules, as Reflected in Kinetic Analysis
… relative to singly-marked transformants. Kinetic analysis has been made of the fate of each factor in a linear array of three genetic markers within one DNA particle. The linear rate of accumulation in time of each of the several kinds of recombinant progeny acquiring any one, two, or three of …
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The role of Decapentaplegic (Dpp) in Drosophila wing development
… normal cell segregation at the A/P boundary by clonal analysis. Third, I investigated whether cross talk among the Hh, Dpp and Wg signaling pathways exists and what its relevance for wing patterning is. To investigate the role of Dpp in Drosophila wing development, the general strategies are to …
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Novel Regulators of Somatic Cell Reprogramming
… expression late in the maturation phase. Clonal analysis of reprogramming cells revealed subsets of stabilization competent (SC) versus stabilization incompetent (SI) cells. SC clones robustly entered the pluripotent state upon transgene withdrawal in the late, but not early maturation …
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Histone Demethylase LSD1 Restricts the Size of the Germline Stem Cell Niche in Drosophila Ovaries
… demethylase exhibit GSC-like tumor formation. Clonal analysis, cell-type specific knock down and rescue experiments demonstrate that Lsd1 functions within the escort cells that reside immediately adjacent to cap cells (niche). Loss of Lsd1 causes the escort cells to adopt an intermediate fate …
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An Integrative Analysis of Fruit Shape in Capsella rubella and Arabidopsis thaliana
… has an elaborate heart-shaped fruit. I used clonal analysis to generate a description of regional growth dynamics within the tissue. Using this data, I generated computational models to explore the coordination of growth and polarity in the Capsella fruit. Based on observations of morphology, …
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Discovery of Immunogenic Neo-peptides from Actionable RNA Fusion CD74-NRG1 for Developing Cancer Vaccines
… exhausted, and effector memory CD8 T cells. Clonal analysis found 21 distinct CD8 T cell clones expanded by 6 of the top binding neopeptides which were validated to be immunogenic through the ELISpot assay. Conserved amino acid residues in the CDR3 sequences of these TCRs supported their …
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Fgf4 and Wnt5a/Pcp Signaling Promote Limb Outgrowth by Polarizing Limb Mesenchyme
… cell migration and/or cell polarity. Our analysis revealed that there was no difference in cell migration when cells were exposed to an exogenous Wnt5a source. However, this did not rule out the possibility that cells were responding in a more mild fashion and polarizing toward a Wnt5a …
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Cellular and genetic mechanisms of new tissue production in the regenerating planarian Schmidtea mediterranea
… these models experimentally, methods for clonal analysis were developed here and used to assess descendants of individual neoblasts. Individual cells, termed "Clonogenic Neoblasts" (cNeoblasts) were identified that gave rise to large descendant cell colonies in vivo. Individual cNeoblasts …
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Identification of pluripotent stem cells and characterization of glia in the planarian Schmidtea mediterranea
… type. We developed two methods for performing clonal analysis to determine the potential of neoblasts: sublethal irradiation and single-cell transplantation. Colonies consisting of both self-renewed neoblasts expressing stem cell markers and differentiated cells expressing specialized tissue …
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Development of a Neoepitope Prioritization Platform to Identify Immunogenic Neoantigens from Oncogenic FGFR3-TACC3 Fusion in Glioblastoma
… and convergence without evidence of oligoclonal dominance. Clonal analysis further identified antigen-responsive CD8 T cell populations with conserved CDR3 features, supporting structural stability and antigen specificity. Collectively, this work establishes a scalable and reproducible …
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Alveolar stem cell dynamics and regulatory mechanisms in homeostasis and early oncogenesis
… transformation. By integrating immunofluorescent analysis with 3D reconstruction and mathematical modelling of AT2- derived clones, I describe the presence of two independent AT2 subpopulations regulated by distinct dynamics. I show that these populations undergo neutral competition with their …
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Identification of a Novel Regulator of ER Stress-Induced Apoptosis
… particular, the use of the FLP/FRT system allows clonal analysis of mutant genes in dispensable tissues of the fly when a gene cannot be easily studied in the homozygous condition. Eye imaginal discs, dissected from the progeny of selected p-element lethal FRT allele males mated to females with …
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Genetic diversity and population structure of Vaccinium vitis-idaea
… crops and their wild relatives, which are often clonal. This is because in species capable of asexual reproduction, it is necessary to first differentiate between clonal ramets and unique genets before measuring genetic diversity. We assessed the clonal structure and genetic diversity in wild …
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Cell Fate Decisions in the Developing Lung
… To address these questions I have taken a clonal lineage-tracing approach to label lung tip epithelial progenitors. A range of tamoxifen doses were administered to two different mouse models, representing either a biased (tip cells only) or unbiased (random) labelling approach, to ensure an …