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Showing 1 to 20 of 171 for “"Cleave"”.
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Designing a mechanism to cleave silicon wafers
… was designed and manufactured to precisely cleave silicon wafers. Two vacuum chucks were designed to support a 150 mm diameter silicon wafer and cleave it by providing a pure moment at a pre-etched v-notch while a vacuum was created on either side of the cut. The design of the vacuum chucks …
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The Ogbanje in Little Bee by Chris Cleave
<p>"In Chris Cleave's 2008 novel Little Bee, he offers readers a literary representation of the Nigerian spirit-child for the purpose of political and post-colonial examination. Cleave's intentions are revealed through a full study of this traditional Nigerian concept. The Nigerian spirit-child, …
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Methods and applications of using DNA-cleaving DNAzymes to cleave double-stranded DNA
Submission published under a 24 month embargo labeled 'Closed Access', the embargo will last until 2024-12-01
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A Cleave within the Piney Woods: Nacogdoches, Stephen F. Austin State University and How Racial Integration Divided the Town and Gown
<p>Stephen F. Austin State University was once the pride and joy of the city of Nacogdoches, Texas. When the Texas State Legislature began to look for a location for their new state normal school, the people of the East Texas town fought to have it built there and the Stephen F. Austin Teacher’s …
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In Vitro Characterization of Proglucagon Cleavage by PCI
… Propeptide converting enzymes recognize and cleave their respective propeptide substrates on the carboxy-terminal side of specific cleavage sites identified most commonly by dibasic residues and less often by tetrabasic and monobasic residues. Identification of a furin/propeptide convertase …
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Quantitative non-destructive study of sub-micron defects in very large scale integrated-circuit silicon using transmitted and reflected infra-red laser scattering
… were then measured by the OPP, the SIRM, and the cleave-and-etch technique and compared with the TEM measurements. In the second sample the defect density was measured only by the cleave-and-etch technique and then by the SIRM"--Abstract, page iii.
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Cystic fibrosis gene repair: correction of ΔF508 using ZFN and CRISPR/Cas9 guide RNA gene editing tools
… corrected using a pair of existing ZFNs, which cleave in intron 9, and the donor repair plasmid pITR-donor-XC, which contains the correct CTT sequence and two unique restriction sites. HDR was initially determined to be <1% but further analysis by next generation sequencing (NGS) revealed HDR …
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ΜΕΛΕΤΗ ΤΗΣ ΠΑΡΑΓΩΓΗΣ IGA1 ΠΡΩΤΕΑΣΗΣ ΑΠΟ MYCOPLASMA ΚΑΙ UREAPLASMA ΑΝΘΡΩΠΟΥ ΚΑΙ ΖΩΩΝ
… UREAPLASMA ISOLATED FROM NONHUMAN HOSTS DID NOT CLEAVE HUMAN IGA1. TWO STRAINS OF CANINE UREAPLASMA WERE ABLE TO CLEAVE CANINE IGA BUT NOT MURINE IGA. THUS UREAPLASMA FROM OTHER SPECIES CAN PRODUCE IGA PROTEASE, BUT THE SPECIFICITY OF THE ENZYME WAS RESTRICTED TO THE IGA OF THE APPROPRIATE HOST. …
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RESPONSE OF A NEIL1 DEFICIENT MURINE EPITHELIAL CELL LINE TO CHROMATE
… glycosylase has been shown to recognize and cleave the oxidative DNA lesion Sp. NEIL1 belongs to a family of three mammalian DNA glycosylases Neil1, Neil2, and Neil3. NEIL1 and NEIL2 are both able to recognize and cleave the oxidative lesion Sp in single stranded DNA in vitro. In duplex DNA, …
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RESPONSE OF A NEIL1 DEFICIENT MURINE EPITHELIAL CELL LINE TO CHROMATE
… glycosylase has been shown to recognize and cleave the oxidative DNA lesion Sp. NEIL1 belongs to a family of three mammalian DNA glycosylases Neil1, Neil2, and Neil3. NEIL1 and NEIL2 are both able to recognize and cleave the oxidative lesion Sp in single stranded DNA in vitro. In duplex DNA, …
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Mechanistic insight on a chimeric Cas9 protein's specificity for DNA target with 5 '-NAA-3' PAM
… with the S. pyogenes Cas9 scaffold to cleave target DNA with an NAA PAM. We show that SpySmacCas9 does not bind or cleave at rates comparable to Casl2a, but its overall performance rivals that of wild-type SpyCas9 with a new PAM preference.
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Host Entry Factors for Human Coronaviruses
… II transmembrane serine proteases, was shown to cleave the spike glycoprotein on human coronaviruses, readying the virus for entry into the target cell. I explored the role of this protein in a cell culture mimic of the human respiratory tract, the primary site of infection. Using Calu-3 cells, I …
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The role of unfolded states in collagen degradation
… exist in the sequence of collagen, only one is cleaved in triple helical collagen. The hypothesis of this work is that locally unfolded states exist in collagen in the absence of collagenases. They occur as a result of the natural thermal fluctuations in the structure of collagen. Collagenolysis …
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Comparison of Extraction Techniques for Δ-9-Tetrahydrocannabinol from Urine
… solution or β-glucuronidase has been used to cleave the bond between the Δ-9-THC and glucuronic acid before an alkaline extraction. Δ-9-THC has a pKa of 10.6 and therefore is always extracted at an alkaline pH. In this study, both a strong solution ofNaOH and the enzyme were used to break the …
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Characterization of IntDOT, the CTnDOT Integrase
… in vivo. The ability of the mutant proteins to cleave and ligate CTnDOT attachment site (attDOT) DNA in vitro in general paralleled the in vivo results. I also showed that the predicted three dimensional structure of the IntDOT core binding domain is similar to that of well-studied tyrosine …
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Biochemical and Structural Studies of the Enzymes Involved in RNA Cleavage and Modification
… E3 is a highly specific endonuclease that cleaves a single bond in 16S rRNA between A1493 and G1494. This cleavage results in termination of protein synthesis in the cell. In order to determine whether the nucleotide sequence of the target site accounts for colicin E3's specificity, we …
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Intrinsic Cleavage Inhibition by Thermophilic Type II-S Restriction Endonucleases
… Type II-S restriction endonucleases (IISREs), cleave double-stranded DNA (dsDNA) not governed by sequence specificity, but rather at a fixed distance from their recognition sequence. These enzymes maintain complete cleavage promiscuity, allowing utilization in techniques such as Golden Gate …
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HUMAN CLCA2 IS A P53-DEPENDENT ZINC METALLOPROTEASE AND ITS INTERACTION WITH EVA1 MAINTAINS DIFFERENTIATION OF HUMAN MAMMARY EPITHELIAL CELLS
… amino acid type I transmembrane protein that is cleaved near amino acid 700 to produce a diffusible 100kD product. The N-terminus contains a hydrolase-like domain with well-conserved HEXXH zinc binding amino acid motif that was proposed to cleave the precursor auto-proteolytically. We investigate …
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Mechanism of Permissive Cleavage Activity of TevCas12a
… I-TevI and Cas12a work together to permissively cleave DNA.
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