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Showing 1 to 7 of 7 for “"Clamp loader"”.
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Comparative Studies of Processivity Clamp Loader ATP Site Function
<p>DNA replicases utilize ring-shaped sliding clamps to ensure polymerase processivity. An ATP-dependent clamp loader topologically links the clamp around DNA in a multi-step mechanism. Clamp loaders are ring-shaped pentamers of AAA+ subunits. The pentamer complex contains 3-4 ATP sites, each …
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Molecular and Biochemical Analyses of a Three-Subunit Euryarchaeal Clamp Loader Complex
… requires two processing factors, sliding clamp and clamp loader, to direct rapid and accurate DNA replication. In eukaryotes, the ring-shaped sliding clamp, proliferating cell nuclear antigen (PCNA), encircles double-stranded DNA within its central hole and tethers the DNA polymerases onto …
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Clamp loading on the leading strand during DNA replication and initial reconstitution of sister chromatid cohesion establishment
… factors. One such factor is the sliding clamp PCNA, which acts as both a processivity factor to support fast and efficient DNA synthesis and as a recruitment platform in various genomic stability maintenance processes downstream of DNA replication. Regulation of chromatin bound PCNA is …
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Single -Molecule and Ensemble Fluorescence Studies of Single -Stranded DNA and Replication Proteins
… DNA binding proteins, and the sliding clamp and clamp loader proteins. Fluorescence studies have been widely used to study the properties of biomolecules and here we will use both Fluorescence Polarization Anisotropy (FPA) and Fluorescence Resonance Energy Transfer (FRET) to study these …
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Structural Studies of DNA Replication Proteins by X-Ray Crystallography and Molecular Dynamics
… The second functional complex is the sliding clamp which adopts a ring-shaped structure, composed of either two or three protomers. It confers processivity onto the polymerase subunit by tethering it to the template. The third complex is the clamp loader complex, which loads the sliding clamp …
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Single-molecule studies of bacterial DNA replication and translesion synthesis
… the replicative polymerase Pol IIIα on the β-clamp. Furthermore, we find that the sequential activities of the replication proteins: clamp loader, clamp, and Pol IIIα, are highly organized while the exchange with the translesion polymerases is disordered. This exchange is not determined by …
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Single-molecule and ensemble studies of DNA replication system
… started from ensemble experiments, studying the clamp (PCNA) and the clamp loader (RFC) from the mesophilic archaeon Methanosarcina acetivorans, and then moved to the single-molecule level trying to reconstitute the DNA replication machinery protein by protein. In the first study, by developing a …