Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 20 of 72 for “"Cis-regulatory elements"”.
-
Computational discovery of Cis-regulatory elements in multiple drosophila species
… factors control gene expression by binding regulatory DNA segments called cis-regulatory modules (CRMs). The increasing number of sequenced genomes of multicellular eukaryotes along with high-throughput methods such as whole genome microarray expression data allows for systematic …
-
Discovering Conserved cis-Regulatory Elements That Regulate Expression in Caenorhabditis elegans
… is two-fold:: 1) To catalog all <italic>cis</italic>-regulatory elements within the intergenic and intronic regions surrounding every gene in <italic>C.elegans</italic>: i.e. the regulome) and: 2) to determine which cis-regulatory elements are associated with expression under specific …
-
Characterization of Cis-Regulatory Elements Controlling Repo Transcription in Drosophila Melanogaster
… factors. In 2005, Lee and Jones defined a 4.3 kb cis-regulatory DNA region that recapitulates the endogenous Repo expression pattern dependent on a single Gcm binding site. Within that region, are three different cis-regulatory elements that drive cell-specific expression independent of Gcm …
-
Identification and characterization of conserved regulatory elements by comparative genomics
… is to use fugu to identify conserved noncoding elements (CNEs) in genes expressing in the human forebrain, and to characterize selected CNEs in transgenic mice. To this end, genomic sequences for 50 human genes were aligned with their orthologous sequences in mouse and fugu. 206 CNEs associated …
-
Mechanistic Dissection of Tcf7l1-Dependent Enhancer Decommissioning During Exit from Naïve Pluripotency
… the simultaneous dissolution of the naïve gene regulatory network as well as a redirection of transcriptional response to Wnt/β-catenin signaling. Here, we show that Tcf7l1 functions as a linchpin factor that connects the two outcomes via effects at Tcf7l1-bound cis-regulatory elements in mouse …
-
Chromatin Architecture Aberrations Contribute and Acute Lymphoblastic Leukemia Relapse
… single nucleotide variants that converge on cis-regulatory elements for the FOXA1 gene in primary prostate tumours. We found that deletion and repression of these cis-regulatory elements significantly decreases FOXA1 expression and prostate cancer cell growth by altering the potential of …
-
Investigating the chromatin dynamics of gene activation
Enhancers are cis-regulatory elements which contribute to the activation of gene expression. The spatio-temporal control of gene expression is particularly important during embryonic development, when the expression of developmental regulators is tightly controlled. Sonic hedgehog (Shh) is an …
-
Characterization of Loop Extrusion and Regulatory Elements in IgH Locus Structure and Function
… the Igh locus. Furthermore, we demonstrate that cis regulatory elements regulate accessibility in the Igh locus, which is correlated with rates of V-DJH joining. Collectively, this demonstrates the importance of cis regulatory elements in Igh locus organization and function.
-
Characterising the function and evolution of enhancers in Drosophila
Enhancers are important modular cis-regulatory elements that precisely control the spatial and temporal expression of most genes. Therefore understanding how enhancers work and evolve is crucial for understanding the regulation of most developmental processes in animals. In my PhD project, I …
-
THE DEVELOPMENTAL BASIS AND EVOLUTION OF WING COLOR PATTERN IN BUTTERFLIES
… patterning wing colors, and to characterize the regulatory architecture modulating color pattern-related function of the WntA gene. Our results revealed that WntA is essential to establish multiple pattern elements, even in highly divergent species of nymphalids and papilionids–two distant and …
-
Functional interpretation of cancer-associated genetic variants
… modulate cancer susceptibility. However, the precise molecular mechanisms behind these associations remain largely uncharacterized, creating barriers to understanding the biological processes behind oncogenesis. This thesis presents an integrated computational method for identifying functional …
-
Characterizing the transcriptional regulation of crassulacean acid metabolism in Kalanchoe
… this work focuses on elucidating the cis-regulatory elements and the trans-acting factors governing the transcriptional control of the phosphoenolpyruvate carboxylase gene (Ppc1) in Kalanchoe. Despite this enzyme's importance in catalyzing the primary nocturnal fixation of CO2 in CAM …
-
Characterization of Transcriptional and Post-transcriptional Regulation of lin-42/Period During Post-embryonic Development of C. elegans
… the research undertaken to characterize the cis-regulatory elements and the trans-regulatory factors that control lin-42 expression. We used direct molecular interaction assays (Electrophoretic Mobility Shift Assay, EMSA) (Chapter 2) followed by an RNA interference (RNAi)-based genetic screen …
-
Identification, improved modeling and integration of signals to predict constitutive and altering splicing
(cont.) manipulation of intronic elements that enables fish genes to be spliced properly in mammalian cells; (iii) A computational analysis using EST data, genome sequence data, and microarray expression data of tissue- specific alternative splicing is conducted, which distinguishes human brain, …
-
SPATIOTEMPORAL CONTROL OF THE SALT STRESS INDUCED TRANSCRIPTIONAL RESPONSE IN ARABIDOPSIS
… pipeline to study the biological functions of cis-regulatory elements which are good starting points for the generation of a CRE centered transcriptional network involved in the salt stress response in the Arabidopsis roots. Using this pipeline, I validated the regulatory functions of seven …
-
Development of broadly applicable transgenic tools for the transposon mutagenesis of the red flour beetle, Tribolium castaneum
… non-species-specific, broad-range transposable elements and the establishment of a universal 3xP3 promoter revolutionized insect transgenesis. It overcame the limitations of the germ-line transformation to be restricted to the model organism Drosophila melanogaster. In combination with …
-
Polyamine metabolism in Arabidopsis: Transgenic manipulation and gene expression
… regions of all AtSAMDC genes contain common cis-regulatory elements that are associated with stress responses, developmental regulation, and hormone responses.</p>
-
A Semi-Supervised Predictive Model to Link Regulatory Regions to Their Target Genes
… act as ``information integration hubs" to confer regulatory requirements on a given cell. These non-coding DNA sequences can regulate genes from long distances, or across chromosomes, and their relationships with their target genes are not limited to one-to-one. With thousands of putative …
-
FUNCTIONAL ROLE OF NON-CODING REGULATORY VARIANTS IN NEUROBLASTOMA
… to disease, mainly by residing in functional regulatory regions and ultimately affecting gene expression networks. However, their interpretation is challenging. In our recent work, whole-genome sequencing (WGS) data from 151 neuroblastoma primary tumours identified two clusters of non-coding …
-
Regulation of Brain-Derived Neurotrophic Factor in the Adult Mouse Brain
… bioinformatics study revealed 9 highly conserved elements that are good candidates for cis-regulatory elements of BDNF. In conclusion, the regulation of the BDNF gene appears far more complicated than was previously predicted.
Page 1 of 4