Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"Circulating Tumor Cells (CTCs)"”.
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Characterization of Photoacoustic Flow Cytometry Signals
… to clinically determine the presence of melanoma circulating tumor cells (CTCs). Further investigation was conducted into the morphology of detection signals and how they could be manipulated to allow for further classification. Novel features were extracted from waveforms that appear to have …
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An optofluidic platform for longitudinal circulating tumor cell studies in mouse models of cancer
… for over 90% of cancer-related deaths. Circulating tumor cells (CTCs) that are shed from primary tumors represent the disseminating "seeds" that give rise to distant malignant growths. Despite their importance to metastasis, understanding of their role has been hindered by the extreme …
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Development of High-Throughput Platforms for Single-Cell Analysis
… deal with highly heterogeneous populations of cells where the contributions of individual players cannot be ignored. Single-cell technologies aim to resolve this heterogeneity by analyzing many individual cells in a high-throughput manner. Here we developed two examples of such tools that rely …
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Novel approaches to investigate behaviors of bacteria by atomic force microscopy and circulating tumor cells through microfluidics
… and apparent ingenuity of renegade and intruding cells within the human body present formidable challenges in warding off disease. As the longevity of humans increases, cancer will afflict greater numbers, and if bacteria continue to grow resistant to conventional antibiotics, new treatment …
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Experimental Validation of Heteroclinic Orbit Dynamics in Inertial Microfluidics
… the goal of improving label-free isolation of circulating tumor cells (CTCs) for liquid biopsy applications. CTCs are extremely rare in blood, making their separation both technically challenging and clinically significant. Inertial microfluidics offers a promising alternative to traditional …
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Genome sequencing and phenotypic analysis of single cells in cancer
… genome profiling (~99%) is done on primary tumors; yet, metastatic cancer is attributed to >90% of cancer-related deaths. The underlying challenge is that metastatic cancer is difficult to sample: surgical resections are less common, metastatic biopsies are not part of clinical routine, and …
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Bioengineered surfaces and hydrogels for specific cell capture and release from whole blood
… is no reliable method to release the target cells from the capture surface while preserving their integrity. This work presents the development and evaluation of a functional hydrogel coating that supplements microfluidic capture devices to enable both specific capture and release. The …
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Passivated-Electrode Insulator-Based Dielectrophoretic Chips for Rare Cell Analysis
… One powerful technique for the analysis of cells is the use of dielectrophoresis (DEP) forces for the manipulation of particle movement. DEP is a particle transport phenomenon, induced by the presence of non-uniform electric fields. The dependence on intrinsic electrical properties of cells, …
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Cell and nanomaterial-based approaches for diagnosis and chemotherapy of metastatic cancer cells
Metastasis is a multistep process during which tumor cells separate from a primary tumor, penetrate the bloodstream, evade host defenses, and colonize distant organs. This final and fatal step in tumor development is the cause of more than 90% of cancer related deaths. Therapies and diagnostics can …
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Zein films as substrates for cell attachment
Circulating tumor cells (CTCs) are cells that have shed into the vasculature from a primary tumor and circulate in the blood stream. Circulating tumor cells play an important role in metastasis. The long term goal of this project is to capture and remocve CTC’s from the blood stream. Numerous …
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Fluid Shear Stress Effects on Cancer Metastasis
… to all metastatic events but its effects on circulating tumor cells (CTCs) remains a mystery. It is known that shear stress can act either directly, causing membrane rupture and cell death, or by altering cell phenotype via mechanotransduction pathways, but to what extent these and other …
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Detection of AR gene rearrangements and regulation of AR variant-driven prostate cancer models
… development and homeostasis, but prostate cancer cells are also dependent on AR signaling for growth and survival. AR is the target for most prostate cancer therapies. Inhibiting this signaling axis is associated with progression to the lethal form of the disease, castration-resistant prostate …
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Biological and Clinical Significance of Circulating Tumor Cells in Breast Cancer
Circulating tumor cells (CTCs) represent a unique source of information that might help clarifying numerous aspects of metastasis biology and finding new clinically relevant biomarkers. On the hypothesis that CTCs possess a distinct profile compared to solid primary and secondary lesions, the …
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INFERRING TUMOR PROPERTIES FROM GENE EXPRESSION DATA
… is used to predict the tissue of origin (TOO) of tumor samples, which is relevant in several scenarios such as the diagnosis of cancer of unknown primary (CUP), early cancer detection by means of circulating tumor cells (CTCs) or cell-free RNA (cfRNA) and the identification of mislabelled cell …
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Functional characterization of mobilized tumor cells
… cascade, examining three types of mobilized tumor cells: circulating tumor cells (CTCs), intraoperatively shed tumor cells, and malignant pleural effusions (MPE). We investigated the functional behavior of mobilized tumor cells in order to explain the discrepancy between the number of tumor …
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Technologies for the isolation of circulating tumor cells
Metastasis, the spread and growth of tumor cells from the primary site to distant organs, is arguably the most devastating and deadly attribute of cancer, and is ultimately responsible for 90% of cancer-related deaths. Circulating tumor cells (CTCs) are exceedingly rare cells found in the whole …
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The hybrid epithelial/mesenchymal phenotype and its implications in cancer metastasis
… of cancer-related deaths occur because cancer cells metastasize, i.e. invade the surrounding tissue, travel throughout the body, and form tumors at distant organs. Metastasis is often fueled by Epithelial-to-Mesenchymal Transition (EMT) that enables cells to migrate and invade, and its reverse …
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Tumor cell deformability in the metastatic cascade
During the process of metastasis, tumor cells must undergo changes that enable them to detach from a tumor, exit surrounding tissue during intravasation, enter into circulation and eventually stop at a distant site for extravasation. Here, we measure the physical changes in the deformability of …
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Label-Free Microfluidic Devices for Single-Cell Analysis and Liquid Biopsies
… biological samples. Biomechanical probing of cells through deformability assays provides a label-free method of identifying cell health and monitoring response to physical and chemical stimuli. Bioimpedance analysis is an alternative versatile label-free method of evaluating cell …
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Decoding the RNA cargo of plasma-derived extracellular vesicles in metastatic castration-resistant prostate cancer
… by capturing multiple molecular components circulating in body fluids, such as circulating tumor cells (CTCs), cell-free DNA (cfDNA), cell-free RNA (cfRNA) and extracellular vesicles (EVs). EVs are nanoscale structures surrounded by a lipid bilayer. They carry a rich molecular cargo that …
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