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Showing 1 to 20 of 34 for “"Circular dichroism spectroscopy"”.
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Computational and experimental studies of collagen and related diseases
… is then compared with experimental results from Circular Dichroism spectroscopy. Similar studies are done on mutant peptides of collagen from the Ehlers-Danlos Syndrome type IV.
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Implications For the Role of the Peroxoferric Intermediate In the Second Step of Nitric Oxide Synthase Derived From the Reactivity of P450-Cam With Camphor-5-Oxime and Characterization of the Heme Iron Coordination Structures of Tyrosine- and Histidine-Ligated Heme Enzymes Using Magnetic Circular Dichroism Spectroscopy
… step of the NOS catalytic cycle.</p> <p>Magnetic circular dichroism (MCD) and UV-Visible (UV-Vis) absorption spectroscopy have been used to characterize the heme-iron coordination structure of (A) several tyrosinate-ligated heme enzymes and (B) the high potential (HP) and low potential (LP) heme …
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Antigenic properties of snake venom cardiotoxins
… peptides in various solvents, was determined by circular dichroism spectroscopy, for relating immunological properties to epitope structure. Antiserum was raised against the two proteins, and the reactivity thereof towards the synthetic peptides determined. ...
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Lanthanide Binding Tag Peptides for Selective Separation of Rare Earth Elements
… and anomalous small angle x-ray scattering. Circular dichroism spectroscopy reveals that metal coordination influences peptide conformation and aggregation via hydrophobic and aromatic interactions. This work integrates interfacial science, macromolecular assembly, and coordination chemistry …
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Design and Synthesis of Hyaluronan:RHAMM Interaction Inhibitors
… lactam bridges. The peptides were analyzed by circular dichroism spectroscopy and one stapled peptide exhibited a 4-fold increase in helicity compared to the unstapled sequence and significantly decreased migration, inflammation, and fibrosis in vitro. This cyclic peptide is a novel …
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Enantioselective Adsorption of 1,3-Dithiolane-1-Oxides and Related Compounds on Liquid Chromatographic Amino Acid-Derived Chiral Stationary Phases (Resolution, Enantiomers, Sulfoxides)
… Confirmation of these assignments is provided by circular dichroism spectroscopy, NMR spectroscopy in the presence of a chiral solvating agent and preparation of chiral sulfoxides from compounds of known absolute configuration. The chiral recognition models are useful for determining the essential …
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The Mechanism of Activation of Potassium Dependent and Potassium Independent Asparaginases from Common Bean (Phaseolus vulgaris)
… in PvAspG1 and PvAspG-T2-I117S, as determined by circular dichroism spectroscopy. However, no change in conformation was observed for PvAspG-T2 and PvAspG1-S118I. The presence of the serine residue was associated with a narrow pH profile, with a sharp peak in enzyme activity between pH 6.5-7 and …
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A Survey of Stapling Methods to Increase the Affinity, Activity and Stability of Ghrelin Analogues
… measured using competitive binding assays and circular dichroism spectroscopy, respectively. Generally, an increase in alpha-helicity using a flexible staple linker led to an improved affinity towards GHSR. Ghrelin(1-20) analogues with a lactam, triazole, and hydrocarbon staple resulted in …
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Human Serum Albumin Characterisation and Binding Studies by Spectroscopic Techniques
… and native HSA using Ultraviolet (UV) & circular dichroism spectroscopy (CD) and involved HSA conformational changes, which altered the ability to bind or off-load ligands. Changing environment, together with the use of characteristic "marker ligands," influences binding to provide a …
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Intrinsic Flexibility of West Nile Virus Protease: In Solution Characterization
… changes due to substrate binding, we performed Circular dichroism spectroscopy, fluorescence experiments, small angle X-ray scattering (BioSAXS) and Fourier transform infrared spectroscopy (FT-IR) experiments. Our results suggested that in absence of a substrate analogous, the NS3pro domain …
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Complexation Between DNA and Hydrophilic-Cationic Diblock Copolymers
… - measured using isothermal calorimetry and circular dichroism spectroscopy, respectively - turn out to show a strong dependence on the cationic content in the diblock, leading to wide ranges of polyplex size, dispersity, and stability. Second, the effects of hydrophilic block structure, …
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Peptide nanovesicles: supramolecular assembly of branched amphiphilic peptides
… of SDS, urea and trypsin as confirmed by circular dichroism spectroscopy. Given sufficient time, they fuse together to form larger assemblies and trap compounds of different sizes within the enclosed space. They are prepared using a protocol that is similar to preparing lipid vesicles. We …
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Porphyrin-DNA as scaffold for nanoarchitecture and nanotechnology
… of techniques such as UV?vis and fluorescence spectroscopy, UV?vis and fluorescence melting studies, circular dichroism spectroscopy and EPR (electron paramagnetic resonance) spectroscopy showed electronic interaction between porphyrins stacked on DNA. Metallation with zinc or copper of …
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A novel bioactive peptide QUB-2022 from the skin secretion of Phyllomedusa bicolor
… its secondary structure needs to be confirmed by circular dichroism spectroscopy.<br/> <br/><i>Thesis embargoed until 31st October 2024 </i>
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Helical Pitch, Hydrophobic Binding, Chiral Templation, and Supramolecular Chelation of M-Phenylene Ethynylene Foldamers
… were studied by electron spin resonance (ESR) spectroscopy to determine the helical pitch of folded mPE oligomers. Based on the relative degree of broadening observed in the ESR spectra it was determined that there are approximately six repeat units in a single helical turn of a folded mPE …
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Phenylene Ethynylene Foldamers: Cooperative Conformational Transition, Twist Sense Bias, Molecular Recognition Properties, and Solid -State Organization
… was quantitatively characterized by fluorescence spectroscopy. From these measurements, it was determined that the stability of the helical conformation is linearly dependent on chain length. The addition of nonpolar methyl groups to the oligomeric backbone was found to stabilize the helical …
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Design and synthesis of molecular tools for the ubiquitin proteasome system
… synthesized and ring-closed on the solid phase. Circular dichroism spectroscopy was used to analyse secondary structure, revealing the peptides exhibited a range of 16 - 91% helicity. Further NMR studies demonstrated the more precise helical nature of short helical peptides in solution. …
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Single-Molecule and Ensemble Measurements Inform on the Role of Protein Crowding at Interfaces
… produces protein-nanorod aggregates that exhibit circular dichroism. These aggregates could be applicable to sensitive chirality detection, but the mechanisms by which they form and produce circular dichroism are not well understood. Circular dichroism spectroscopy and scanning electron microscopy …
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Development and Application of 19F NMR of Proteins
… thoroughly investigated using 19F and 1H-15N NMR spectroscopy, 15N spin relaxation and thermal denaturation via circular dichroism spectroscopy. While stability was unaffected, NMR experiments revealed increased protein plasticity, minor conformers and line broadening. The merit of fractional …
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Intracellular localization, biochemical and biophysical properties of human Armet
… for calcium binding by Armet was obtained by circular dichroism spectroscopy (the binding of calcium appeared to decrease helix content), by differential scanning calorimetry (binding of calcium resulted in a less structured protein) and two-dimensional (1H-15N HSQC) nuclear magnetic resonance …
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