Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 55 for “"Cilium"”.
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Sensory Primary Cilium is a Distinct Signaling Compartment
<p>The primary cilium is a solitary cellular organelle that protrudes from the apical cell membrane. Findings on cilia-dependent mechanosenstation have shown that the primary cilium acts as a transducer of fluid-shear stress into intracellular signaling. Over recent years, studies in primary cilia …
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Artificial Micro-Devices: Armoured Microbubbles and a Magnetically Driven Cilium
… on a wall. Secondly, we study an artificial cilium, which due to its unique design, when placed in an array, easily produces a metachronal wave for fluid transportation. The Armoured Microbubble was designed by our experimental collaborators (group of Philippe Marmottant, University Grenoble …
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Structural and biophysical characterization of membrane-coating proteins from the nuclear pore and the primary cilium
… pathway. Additionally, transport to the primary cilium is not well characterized and is therefore of interest. The nuclear envelope comprises a double membrane that fuses at points to produce pores, which link the nucleoplasm with the cytoplasm. Coating these openings are nuclear pore complexes …
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Cardiac repair post-myocardial infarction: Roles of the primary cilium and the long non-coding RNA Malat1
… factor Wilms’ tumor 1 (Wt1) and the primary cilium regulate EMT. Furthermore, the long non-coding RNA (lncRNA) Malat1 is not only known to regulate EMT, but also mitigates cell death from injury. Little is known about the roles of Wt1, primary cilia, and Malat1 in relation to neonatal cardiac …
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The Protein Composition of the Chlamydomonas Flagellar Membrane Is Dynamically Regulated by Cilium-Generated Signaling During Fertilization
… of cilia and flagella at steady state and during cilium-generated signaling are poorly understood. Our laboratory uses the biflagellated green alga Chlamydomonas as a model system to study regulated movement of the flagellar adhesion receptor SAG1 from the cell body to the flagella. Interactions …
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Multi-Step Trafficking Pathway for Regulation of the Protein Composition of the Chlamydomonas Flagellar Membrane during Cilium-Generated Signaling
The primary cilium is a signaling organelle found on most cell types in vertebrates. It is a distinct compartment for membrane and cytoplasmic proteins that mediate multiple sensory processes. Little is known about the cellular and molecular mechanisms that determine regulated ciliary localization …
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Daple Orients and Patterns Sensory Hair Bundles in the Inner Ear
… hair-bundle development. Although the primary cilium also participates in each of these pathways, the mechanism that links tissue-wide and cell-intrinsic polarity is not yet understood. To address this issue, I initiated a yeast two-hybrid screen with a central component of the cell-intrinsic …
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Function of the forkhead gene fd3F in Drosophila chordotonal neuron differentiation
… of the Ch neuron is a compartmentalised motile cilium, a feature that is exclusive to this neuron subtype. Ch organs are specified early in development by expression of the proneural gene atonal in the proneural cluster and sense organ precursors (SOPs) (Jarman et al., 1993). However little is …
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Regulation of Inverted Formin-1 (INF1) by Microtubule-Affinity Regulating Kinase 2 (MARK2)
… INF1 overexpression is associated with primary cilium absence and in some cases, the presence of long cilia, suggesting that INF1 plays a role in primary cilium formation.
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Targeting Primary Cilia Immune Receptor Proteins for the Treatment of Polycystic Kidney Disease Mechanisms
… health, and others genetic diseases. Primary cilium functions as a mechano-sensor and chemo-sensor. Defect in primary cilia causes the progression of polycystic kidney disease (PKD) which further leads to the inflammatory responses. We, therefore, investigated the role of Toll-like receptors 4 …
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Stiffness controls primary cilia position and length: mechanisms and potential in reversing osteoarthritis phenotypes
… through the action of, the primary cilium. Mechanical stress and the stiffness of the extracellular matrix are key drivers of tissue development and homeostasis. Aberrant mechanosensation is associated with a wide range of pathologies, including diseases such as osteoarthritis (OA). …
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Mutation of Polaris, an Intraflagellar Transport Protein, Shortens Neuronal Cilia
… of polaris in the development of neuronal cilia, cilium length and numerical density of cilia were quantitatively assessed in six different brain regions on postnatal days 14 and 31 in Tg737orpk mutant and wildtype mice. Our results indicate that the polaris mutation leads to shortening of cilia …
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Chemically Based Interrogation of Smoothened, a Proto-Oncogenic Component of the Hedgehog Signal Transduction Pathway
… antenna-like structure known as the primary cilium. Furthermore, this deviant form of signaling from an intracellular compartment shelters SmoM2 from chemical attack thus rendering it intrinsically resistant to certain Smo antagonists. Based on this new understanding of SmoM2 signaling, I was …
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The Role of Peripherin in Photoreceptor Outer Segment Morphogenesis
… which both contain a highly evolved sensory cilium called the outer segment. The outer segment is a specialized cellular compartment that contains all of the protein machinery involved in converting the initial light signal into an electrical signal that can be ultimately transmitted to the …
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Dynamic and structural basis of complex behaviour in a ciliated unicellular organism
… we develop a macroscopic robotic model of the cilium. Taking inspiration from theoretical models of dynein coordination, our two filament design incorporates actuation along the robo-cilium length and relies purely on mechanical coupling to organise the shape dynamics. We discuss how such …
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A functional and therapeutic investigation of ciliopathy proteins and ciliopathies
… diseases whose aetiology lies in the primary cilium. Over 30 genes have been identified as mutant in ciliopathies and their proteins localise at the primary cilium. When mutated they can cause kidney disease, obesity, polydactyly, and retinal degeneration. In this project, I have studied …
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The role of CD133 in mouse incisor tooth epithelium
… recruitment of Arl13b and Hdac6 to the primary cilium regulating proper ciliogenesis. Establishment of an in vitro cell culture model of dental epithelial cells allowed the investigation of nuclear CD133 under normal physiological conditions. To date, nuclear CD133 has been reported in a variety …
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Immunohistochemical characterization of neuronal cilia in the rat central nervous system.
… negative correlation between a region's mean cilium length and that region's distance to the nearest ventricle; regions nearest ventricles were those with the longest cilia. This correlation suggests neuronal cilia may function as chemosensors, detecting substances as they move out from the …
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Understanding HNF-1β through Identification of Interacting Proteins and Target Genes in the Kidney
… showed that PDE4C is localized in the primary cilium and there interacts with a protein complex containing AKAP150, adenylyl cyclase 5/6 (AC5/6), protein kinase A (PKA), and polycystin-2 (PKD2). These results identify a cAMP-regulating protein complex that is localized in the primary cilium and …
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Defining the molecular composition and organisation of Transition fibres in Trypanosoma brucei and their roles in flagellar assembly and length control
The eukaryotic cilium/flagellum is a slender microtubule-based projection that extends, with a contiguous membrane, out from the cell body. They possess a highly conserved core structure, composed of a microtubule-based axoneme that runs through its core. The axonemal microtubules are nucleated by …
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