Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"Chromatin remodelling"”.
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Nitric oxide induces chromatin remodelling in the developing central nervous system
… broad level of gene regulation by influencing chromatin remodelling. My data also show that NO accumulates within the nucleus of cortical neurons upon BDNF stimulation, thereby inducing Snitrosylation of a wide array of nuclear proteins. S-nitrosylation of histone deacetylase 2 (HDAC2) …
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Genetic interactors of the SWI/SNF chromatin remodelling complex in Caenorhabditis elegans
Chromatin remodellers are adenosine triphosphate (ATP)-powered molecular machines that can directly alter the structure of chromatin by reshuffling or evicting nucleosomes. Thereby they control the access to DNA elements like promoters and replication origins that need to be exposed to execute …
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Role of host chromatin remodelling in Mycobacterium leprae-induced Schwann cell fate change
… Sox2 binding sites in order to define changes to chromatin accessibility and Sox2 binding. Cultured mSwCs, along with fixed ML-mSwC models, were validated and phenotypically characterised through microscopy with additional immunofluorescence staining for Schwann cell lineage markers (p75-NTR, …
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Investigating the role of SWI/SNF chromatin remodelling complex in Clear Cell Renal Cell Carcinoma
SWI/SNF related ATP-dependent chromatin remodelling complexes act to regulate chromatin accessibility, ensuring that genes are regulated appropriately. Components of the complexes are mutated in approximately 20% of human cancers. PBRM1 is one of defining subunits of the PBAF subtype of SWI/SNF …
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The role of the SWI/SNF ATP dependent chromatin remodelling complex in the regulation of the human hair follicle cell proliferation and control of the human cutaneous wound healing
… nucleosome positioning and ATP-dependent chromatin remodelling as well as higher order chromatin folding and 3D genome organisation. ATP-dependent chromatin remodelling complexes modulate nucleosome structure, positioning and chromatin de-compaction and are involved in gene activation and …
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Mechanisms of epigenetic regulation in epidermal keratinocytes during skin development. Role of p63 transcription factor in the establishment of lineage-specific gene expression programs in keratinocytes via regulation of nuclear envelope-associated genes and Polycomb chromatin remodelling factors.
… envelope-associated proteins, changes in heterochromatin distribution as well as decrease of the expression of several polycomb proteins (Ezh2, Ring1B, Cbx4) has been observed in p63-null keratinocytes. Moreover, recent data in our lab have showed that p63 directly regulates Cbx4, a component of …
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Investigating the mechanisms of action of the SNF2-homolog protein Fun30
In eukaryotes, the manipulation of chromatin structure represents a means of regulating access to genetic information. One way in which this can be achieved is via the action of ATP-dependent chromatin remodelling complexes related to the S.cerevisiae Snf2 proteins. The Fun30 protein (Function …
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Epigenetic control of seed dormancy using Capsella bursa-pastoris as a model species
… which is often associated with major changes in chromatin structure. Recent advances have highlighted the role of chromatin remodelling in dormancy regulation. Despite the high importance of dormancy for plants competitiveness and life cycle timining, there is an enourmous lack of knowledge about …
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The role of the chromatin remodeller ISWI in the cellular response to hypoxia
… access sequences in DNA that are integrated in chromatin. It is known that the chromatin remodelling complex SWI/SNF (Switch/Sucrose non-fermenting) is essential for HIF activity. However, no additional information exists about the role of other chromatin remodelling enzymes. This thesis …
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Role of ATRX in colorectal cancer metastasis
… mental retardation x-linked (ATRX) is a chromatin remodelling protein with established roles in regulating chromatin conformation, genomic stability, and homologous repair. ATRX is frequently mutated in many cancer types and in colorectal cancer (CRC) loss of ATRX is associated with the …
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Structural Investigation of the Chromatin Remodeller Fun30
Manipulation of chromatin structure is closely linked to the process of gene regulation. The core component of chromatin, the nucleosome, consists of approximately 147 base pairs of DNA wrapped around a protein core consisting of two histone H2A-H2B dimers and a histone H3-H4 tetramer. Chromatin …
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SMARCB1 Maintains Lineage Fidelity in Clear Cell Renal Cell Carcinoma
… the loss of SMARCB1, a member of the SWI/SNF chromatin remodelling complex, confers an advantage to ccRCC cells upon inhibition of the essential renal lineage factor PAX8. SMARCB1 knockout (KO) leads to large-scale loss of a kidney-specific enhancer program, conversion to a cellular state …
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Engineering Cell Lines to Investigate the Function of Mammalian SWI/SNF Complexes
… SWI/SNF complexes belong to the class of chromatin remodelling complexes, and consist of approximately 15 subunits. Bioinformatics analyses of tumour sample sequencing studies have shown that members of the mSWI/SNF complexes are mutated in almost 20% of all cases, suggesting many subunits …
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The impact of ARID1A loss on ER+ breast cancer: mechanistic insights and therapeutic opportunities
… in ARID1A, a critical subunit of the SWI/SNF chromatin remodelling complex. In 2020, Nagarajan et al. have shown that ARID1A mutations contribute to resistance to endocrine therapy in breast tumours while simultaneously increasing their sensitivity to epigenetic BET inhibitors. This project …
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Epigenetic Regulation of Skin Development and Postnatal Homeostasis The role of chromatin architectural protein Ctcf in the control of Keratinocyte Differentiation and Epidermal Barrier Formation
… However, mechanisms that regulate higher-order chromatin remodelling and transcription of keratinocyte-specific genes that are clustered in the genome into three distinct loci (Keratin type I/II loci and Epidermal Differentiation Complex (EDC) during differentiation of the epidermis are poorly …
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Studying the Clonal Origins of Drug Resistance in Human Breast Cancers
… quiescent repopulation dynamics, drug induced chromatin remodelling or differential transcription factor binding, that can be transient or permanent in nature. This thesis will outline experiments using high complexity molecular barcodes to trace the fate of individual cellular clones in the …
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Contribution of Lsh to DNA methylation reprogramming in embryonic stem cell, epiblast stem cell and embryoid body model systems
… Lsh (lymphoid specific helicase), a putative chromatin remodelling helicase, has been implicated in facilitating de novo methylation, as Lsh knockout embryos and derived somatic cell lines display substantial but specific DNA methylation losses at repetitive elements and single copy genes. …
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Molecular mechanisms of pioneer factor ELF2 interactions with the nucleosome
… and regulate gene expression at closed chromatin regions. Pioneer factors are important for stem cell pluripotency, cell reprogramming and differentiation. ETS family TFs are a large subfamily of TFs consisting of 29 members. The DNA binding domains (DBD) of ETS family TFs are highly …
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Understanding the relationship between large-scale chromatin structure and gene expression
… into a complex DNA, RNA, and protein rich chromatin fibre, creating an interdependent functional relationship between the structure of the chromatin and the activity of the genetic features stored within. The composition of active genomic regions is weighted towards transcription factors, …
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