Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 61 for “"Chromatin immunoprecipitation (ChIP)"”.
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Estrogen Regulation of Proteinase Inhibitor 9 Gene Expression and Interleukin-1beta Production
… with the native PI-9 promoter we carded out chromatin immunoprecipitation (ChIP) assays. The ChIP assays demonstrated that moxestrol-ER complex bound to the PI-9 promoter, but that OHT-ER (and unliganded ER) did not bind. These data provide the first evidence that the agonist activity of …
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Microfluidic Engineering for Ultrasensitive Molecular Analysis of cells
… and fast molecular analysis of cells. Chromatin immunoprecipitation (ChIP) assay followed by next generation sequencing serves as the primary technique to characterize the genomic locations associated with histone modifications. However, conventional ChIP-seq assay requires large …
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The Sumoylation of General Transcription Factors Regulates Transcription in Saccharomyces cerevisiae
… or reduced TFIID or TFIIF sumoylation. Using chromatin immunoprecipitation (ChIP), we found that GTF sumoylation does not substantially impact GTF-chromatin interactions but can impact the promoter-wide sumoylation level. Notably, TFIID and TFIIF sumoylation enhances RNAPII recruitment at …
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Reproducibility and reliability of chromatin immunoprecipitation in clinical research
… and epigenetic silencing. Changes to chromatin structure influence gene expression via histone modifications. Chromatin immunoprecipitation (ChIP) is an experimental technique used to investigate the interactions between histones and DNA. ChIP determines the specific location in the …
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Recording Transcription Factor Binding During Development
… named "Calling Cards", offers an alternative to chromatin immunoprecipitation (ChIP) methods and furthermore has the ability to record transcription factor binding through cell division and development. I first demonstrated the method using the Ty5 transposon in the budding yeast Saccharomyces …
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Practical Approaches to Biological Network Discovery
… how network inference results can complement chromatin immunoprecipitation: ChIP) experiments, which directly probe the binding events of transcriptional regulators. Collectively, my contributions improve both the accessibility and practicality of network inference methods.
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Regulation of flagellin glycosylation genes in Campylobacter jejuni: influence of NssR, the nitrosative stress response regulator.
… of the affected genes was deduced and chromatin immunoprecipitation (ChIP) used to map NssR binding to six potential locations in the region. Methods to measure bacterial motility were assessed and used to document the defective motility of the nssR mutant, which was found to be due to …
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MECHANISM OF TRANSCRIPTIONAL REPRESSION OF MMP-9 BY INTERFERON-β
… and -72, which contains an AP-1 binding site. Chromatin immunoprecipitation (ChIP) studies indicate that IFN-beta increases histone deacetylase (HDAC)-1 recruitment to the MMP-9 promoter and reduces histone H3 acetylation, in addition to reduced NF-kappaB recruitment. Accordingly, IFN-beta's …
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Bmp-Signaling Regulates A Common Transcriptional Program to Control Facial Form and Skeletal Morphogenesis
… modulation and normal skeletal morphogenesis. Chromatin immunoprecipitation (ChIP) revealed a subset of the BIG signature, including <em>Satb2, Smad6</em>, <em>Hand1, Gadd45</em><em>g</em> and <em>Gata3</em> that was bound by Smad1/5 in the developing mandible revealing direct, Smad-mediated …
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Investigation into the molecular mechanisms underlying circadian rhythm disruption and human cancer
… electrophoretic mobility shift assays (EMSA) and chromatin immunoprecipitation (ChIP) experiments. At the cellular level, Trp53YC luciferase reporter cells were found to have dampened circadian oscillations, altered rhythmicity of core clock gene transcription, and exhibited increased …
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A study of the composition and function of telomeric chromatin in drosophila melanogaster.
… of the proteins that constitute telomeric heterochromatin. Genetic dissection was used to exploit this phenomenon in order to identify components of telomeric heterochromatin in Drosophila melanogaster. Using genetic dissection, followed by a chromatin analysis technique known as Chromatin …
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Autonomous and non-autonomous regulation of chromatin structure during cellular senescence
… NOTCH1 signalling confers a dramatic impact on chromatin structure during senescence. RAS-induced senescent (RIS) fibroblasts often develop chromatin structures called senescence-associated heterochromatic foci (SAHF). We find that NOTCH1 inhibits SAHF formation at least partially through …
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Histone Demethylase LSD1 Restricts the Size of the Germline Stem Cell Niche in Drosophila Ovaries
… as a direct target of Lsd1 by performing Chromatin Immunoprecipitation (ChIP-seq) analysis in the escort cells of the Drosophila ovary. Engrailed is expressed in the cap cells of wild type germaria and in Lsd1 mutants engrailed transcripts are misexpressed in the escort cells. Knocking …
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Delineating the Upc2A Regulon in Candida glabrata
… an activating mutation (GOF) in UPC2A. Global chromatin Immunoprecipitation (ChIP-seq) was used to identify genes whose promoters were bound by Upc2A in strains carrying WT and GOF alleles of Upc2A with and without fluconazole exposure. Only three genes (UPC2A, ERG25, and ERG3) were found to be …
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Genomic Approaches for Pathway Identification in Regenerating Sensory Epithelia of the Inner Ear
… with two of these targets: LMO4 and MBNL2) by chromatin immunoprecipitation: ChIP) using GATA3 antibodies and also demonstrated by RNA in situs that both these genes exhibit patterns of expression consistent with their direct regulation by GATA3.
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HP1BP3, A Chromatin Retention Factor for Co-Transcriptional MicroRNA Processing
… the Drosha-DGCR8 complex can be recruited to chromatin to catalyze co-transcriptional processing of primary microRNAs (pri-miRNAs) in mammalian cells. However, the molecular mechanism of co-transcriptional miRNA processing is poorly understood. Here, we find that HP1BP3, a histone H1-like …
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Epigenetic Patterns: Process-Driven Or Sequence-Driven?
… (RH-43), or a full late promoter region (T-165). Chromatin immunoprecipitation (ChIP) and real-time quantitative polymerase chain reaction (q-PCR) of the histone modifications H3K4me1/2/3, H3K9me1/2/3, H4K20me1 and RNAPII were analyzed. We found that certain modifications were affected by each …
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Mechanism of Chimeric Vaccine Mediated Immune Suppression of Human Dendritic Cells
… inhibited IDO1 expression in human DCs. Chromatin immunoprecipitation (ChIP) analysis, showed that RelB-p52 dimers bound dendritic cell NF-κB consensus sequences in the IDO1 promoter region, demonstrating vaccine stimulation of NF-κB non-canonical pathway activation of IDO1 expression in …
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THE APPLICATION OF HISTONE ANALYSIS FOR IDENTIFYING DISTAL REGULATORY ELEMENTS - REGULATION OF FSHR
… states. Genome-wide approaches that combine chromatin-immunoprecipitation (ChIP) and next-generation sequencing have greatly advanced our understanding of chromatin structure and the role that histone modification plays in transcriptional regulation. In particular, these advances revealed …
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A Study on the Effect of Peroxisome Proliferator-Activated Receptors (PPARs) Activation in Cultured Rat Brain Glial Cells
… as key players, which were further confirmed by chromatin immunoprecipitation (ChIP). In an experiment to further reveal the mechanisms involved, we found that the differential effects on AP-1/SP-1 were mediated by Jun N-terminal kinase (JNK) and MAP kinase phosphatase-1 (MKP-1), upstream …
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