Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 14 of 14 for “"Chromatin assembly"”.
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Expression, purification and characterisation of recombinant chromatin assembly factor 1
Chromatin Assembly Factor 1 (CAF-1) is the only known replication dependant histone chaperone, responsible for the deposition of the histone H3/H4 tetramer onto DNA. Found in all eukaryotes, CAF-1 consists of three subunits, p150, p60 and p48. Since its identification work on CAF-1 has mainly …
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Role of Nuclear Hat1p Complex and Acetylation of Newly Synthesized Histone H4 in Chromatin Assembly
… Hat1p has long been presumed to be involved in chromatin assembly through the acetylation of newly synthesized histone H4 on lysine 5 and 12, there has been lack of straightforward evidence linking Hat1p to this process. We utilized the yeast Gal-HO system to study the function of nuclear …
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Investigation of The Roles of Asf1 and Caf-1-Mediated Chromatin Assembly In The Human Dna Damage Response
… access-repair-restore model for the role of chromatin in DNA repair infers that chromatin is a mere obstacle to DNA repair. However, here we show that blocking chromatin assembly of newly-synthesized histones, via knockdown of the histone chaperones ASF1A, CAF-1 or a mutation that …
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Dissecting Mammalian Replication-Independent Chromatin Assembly — Biochemical and Structural Studies on the H3.3-Specific Histone Chaperones Hira and Daxx
… the fundamental packaging unit of eukaryotic chromatin — the nucleosome. Beyond their structural role, they regulate virtually all processes that act on or depend on DNA, such as replication, gene expression and maintenance of centromeres and telomeres. Despite the high conservation of the …
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Genome-Wide Dynamics of Chromatin Maturation Following DNA Replication
… transcription, occur in the context of the local chromatin environment. The passage of the replication machinery results in disassembly of chromatin, which must be re-assembled behind the replication fork to re-establish the epigenetic state of the cell. Many of the factors and mechanisms …
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Genomic distribution of histone H1 in budding yeast (Saccharomyces cerevisiae) : yeast chromosome III
… of nucleosomes into the 30-nm filament of the chromatin. This compaction of DNA has a well-characterized effect on DNA function. In Saccharomyces cerevisiae, HHO 1 encodes a putative linker histone with very significant homology to histone HI. In vitro chromatin assembly experiments with …
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DNA supercoiling with a twist
… of specific DNA metabolic processes as well as chromatin assembly. High mobility group proteins (HMGB) are architectural proteins whose HMG DNA binding domains confer significant preference for distorted DNA, such as supercoiled DNA and 4-way junctions. HMGB proteins play a role in transiently …
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Structural Studies of a Non-Sequence-Specific DNA Binding Protein, Hmg-D, Complexed With DNA
… transcriptional activation and repression to chromatin assembly. These proteins can be viewed as two classes divided by their ability to bind DNA in a sequence-specific vs. non-specific manner: LEF-1 and SRY fall into the former category while the latter is exemplified by HMG-1/2 and by HMG-D, …
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Mechanism of Human Papillomavirus E6-Mediated Repression of P53-Target Gene *Transcription
… inhibitors (MG132) and in vitro-assembled chromatin templates reconstituted with purified chromatin assembly factors (Acf1 and ISWI), core histones (H2A, H2B, H3, and H4), and a histone chaperone (NAP-1) in order to define the mechanism used by high-risk and low-risk HPV E6 to inhibit …
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The structural analysis of histone H3 lysine 56 acetylation and related histone chaperone complexes
… eukaryotic organisms is regulated in part by the assembly and disassembly of chromatin. Key regulators of these processes are histone binding proteins known as histone chaperones. The histone chaperone complex Chromatin Assembly Factor 1 (CAF-1) is responsible for depositing H3.1/H4 tetramers in a …
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MenzelJohannes_MSc_July_2013
… role the APC plays in cell cycle progression, chromatin assembly, genomic stability, stress response and longevity will be valuable to fundamental biological science, and may also have applications in health science and medicine.
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Transcription Factor Dynamics Investigated through Single-Molecule Imaging, High-Throughput Sequencing, and Neural Networks
The general metadata -- e.g., title, author, abstract, subject headings, etc. -- is publicly available, but access to the submitted files is restricted to UT Southwestern campus access and/or authorized UT Southwestern users.
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Investigation of Regulatory Mechanisms of Chemical-Mediated Fruit Thinning in Apple (Malus X Domestica Borkh.)
… biosynthesis, photosynthesis, carbon fixation, chromatin assembly, auxin transport/efflux, cytoskeleton function, and flower development were generally downregulated. Comparison of changes in the gene expression patterns during NAA-induced fruit abscission with shading-induced fruit abscission …
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Small Molecules Modulate Chromatin Accessibility to Promote NEUROG2-Mediated Fibroblast-to-Neuron Reprogramming
… SOX4 expression, and promote SOX4-dependent chromatin remodeling. Genome-wide occupancy analysis revealed that SOX4 targets numerous SWI/SNF complex subunits and co-binds with NEUROG2 to enhance the expression of diverse neurogenic factors. The overexpression of SWI/SNF chromatin remodeling …