Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 20 for “"Chromatin Remodeler"”.
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Maintenance of Genomic Integrity by RSF1 Chromatin Remodeler
PART1 1 I. INTRODUCTION 2 II. MATERIALS AND METHODS 4 A. Cell culture and treatments 4 B. Generation of RSF1 KO cells 4 C. Transfection and RNA interference 4 D. Plasmid and purification of recombinant proteins 5 E. Immunoprecipitation and in vitro binding assay 5 F. In vitro kinase assay 6 G. Cell …
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Analysis of CHD Remodelers during Development: A Tale in Two Organisms
… Organisms have recruited ATP-dependent chromatin remodelers to ensure the correct timing of gene expression during developmental transitions. Here I show how different CHD ATP-dependent chromatin remodelers regulate developmental transitions of different organisms. I show that PICKLE not …
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A functional role of chromatin remodeling factor, Rsf-1 in the DNA damage signaling pathway
… imitation switch (ISWI) family in ATP-dependent chromatin remodeling factors, RSF complex consists of SNF2h ATPase and Rsf-1. Although it has been reported that SNF2h ATPase is recruited to DNA damage sites (DSBs) in poly(ADP-ribosyl) polymerase 1 (PARP1)-dependent manner in DNA damage response …
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Roles for Chd7 in Zebrafish Development with Implications for Human Disease
… genes, consistent with the hypothesis that this chromatin remodeler is necessary for the proper development of the long axis of the body and may be involved in the onset of human scoliosis. We show that the presence of Chd7 is crucial for proper neural, retinal and vertebral development in …
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An Investigation into the Evolution and Function of RNA-Directed DNA Methylation and its Component Proteins
… of a Pol IV/Pol V associated protein of the chromatin remodeler family in the CGAs. Our results show that homologs of Pol IV and Pol V first and second subunit evolved around the same time point as the Pol IV/Pol V-associated chromatin remodeler homolog that is identifiable in the CGAs, and …
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Novel use of a cell-penetrating peptide-adaptor system to characterize Akirin constructs and investigate activity of bacterial effector proteins
… to interact with transcription factor Twist and chromatin remodeler Brahma. The functional regions of Akirin are unknown; therefore, TAT-CaM was used to characterize the four conserved regions of Akirin. Bacterial effectors, AopP and AvrA, have been shown to inhibit the NF-kB pathway. Previous …
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LINKING CHROMATIN REMODELING TO MITOCHONDRIAL DYSFUNCTION: A ROLE FOR THE SMARCA5-MYC-TFAM AXIS IN HEPATOCELLULAR CARCINOMA
… Matrix Associated, Actin Dependent Regulator of Chromatin, Subfamily A, Member 5), an ATP-dependent chromatin remodeler of the ISWI family, as essential gene for maintaining mitochondrial function, proliferation, and viability in HCC cells. Depletion of SMARCA5 led to severe impairments in …
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Epigenetic Regulation of Oligodendrocyte Development and Regeneration in the Central Nervous System
… and remyelination. First, we show that chromatin remodeler Chd7 is required for proper onset of CNS myelination and remyelination. Genome-occupancy analyses, coupled with transcriptome profiling, reveal that Chd7 interacts with Sox10 and targets the enhancers of key myelinogenic genes, …
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Arid1a Haploinsufficiency Initiates Neural Crest Transformation in a Mouse Model of Mycn-driven Neuroblastoma
… tumors have uncovered precise deletions in the chromatin remodeler and tumor suppressor gene (TSG) ARID1A. Additional causal studies supported ARID1A’s candidacy as a putative 1p36 TSG in MYCN-driven NBL. This study aimed to causally test Arid1a loss during Mycn-driven NBL initiation through the …
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Regulation of Flo11p-dependent adhesion in <i>Saccharomyces cerevisiae</i>
… with genes for components involved in chromatin establishment, maintenance or remodeling identified the histone variant H2A.Z/Htz1p as yet unknown factor that is required to keep FLO11 in a silent state. The chromatin remodeler Rsc1p and the histone acetyl transferase Gcn5p are …
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ROLES OF ACCESSORY DOMAINS IN THE CATALYTIC SUBUNIT OF ISW2
Regulation of chromatin structures in eukaryotes involves ATP-dependent chromatin remodeler complexes. ISW2 belongs to the ISWI family of chromatin remodelers in Saccharomyces cerevisiae and has been shown to be involved in transcription repression and DNA replication. The catalytic subunit of ISW2 …
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Dissecting Mammalian Replication-Independent Chromatin Assembly — Biochemical and Structural Studies on the H3.3-Specific Histone Chaperones Hira and Daxx
… the fundamental packaging unit of eukaryotic chromatin — the nucleosome. Beyond their structural role, they regulate virtually all processes that act on or depend on DNA, such as replication, gene expression and maintenance of centromeres and telomeres. Despite the high conservation of the …
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Unraveling Epigenetic Mechanisms That Regulate Breast Cancer Lung Metastatic Relapse by Rewiring the Immune Microenvironment
… ATAC-seq revealed wide-spread shifts in chromatin accessibility upon Brd7-loss, while ChIP-seq demonstrated alteration of H3K27ac labeling at thousands of genomic sites, with Brd7-loss exhibiting reduced H3K27ac binding at multiple transcription factor sites with key roles in immune cell …
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Dissecting the role of CHD4 in the NuRD and ChAHP complexes
… protein 4) is a widely conserved ATP-dependent chromatin remodeler essential for early mammalian development. Although best known for its role in gene regulation as a core component of the NuRD complex, CHD4 is also found within the ChAHP complex, which contributes to silencing of transposable …
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The roles of DPPA2, DPPA4 and SMARCA5 in mouse zygotic genome activation, epigenetic reprogramming and development
… small DNA binding proteins DPPA2, DPPA4 and the chromatin remodeler SMARCA5 as potential inducers of major ZGA. Here I focus on validating these three factors and I assess their roles in vivo, using conditional knock-out mouse models and a targeted protein depletion system, together with the …
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NOVEL INSIGHTS INTO GENETIC AND ENVIRONMENTAL DETERMINANTS SHAPING GNRH NEURON BIOLOGY AND RELATED REPRODUCTIVE DISORDERS
… variants in the CHD7 gene, encoding a chromatin remodeler essential for neural crest and olfactory system development. CS is characterized by a range of anomalies, including craniofacial malformations, sensory deficits, and hypogonadotropic hypogonadism. Despite CHD7 mutations being the …
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MODES OF NUCLEOSOME INTERACTION AND MECHANISMS OF THE SACCHAROMYCES CEREVISIAE CHROMATIN REMODELERS INO80 AND ISW1A
The dynamic nature of eukaryotic chromatin enables the packaging of large amounts of genetic material in a small space. At the same time, it provides controlled access to genomic DNA for a variety of nuclear processes for example, transcription and DNA repair. The transition between open and closed …
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STRUCTURAL AND FUNCTIONAL ANALYSIS OF THE ISW2 CHROMATIN REMODELING COMPLEX
Chromatin remodelers utilize the energy derived from ATP hydrolysis to mobilize nucleosomes. ISWI remodelers mobilize and evenly space nucleosomes to regulate gene expression. ISW2, an ISWI remodeler in yeast, has been shown to reposition nucleosome near promoter regions and represses both mRNA and …
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Single molecule investigations into DNA replication, recombination and repair
Double-stranded DNA breaks (DSBs) is a principal cytotoxic damage that can be repaired by several well-known repair pathways. One of the most prominent routes is the homologous recombination (HR). The mechanism of repair has been studied primarily by biochemical analysis over the years. Recent …