Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Chromatin Profiling"”.
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Exploiting Lentiviral Integrations to Profile Chromatin
Current methods for profiling histone modifications and protein localisation along chromatin suffer from critical limitations: the gold standard, ChIP-seq, relies on high-quality antibodies, while other antibody-free techniques like DamID are limited by the availability of specific DNA sequences. …
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Identifying CDK8/19 as a Novel Regulator of EWS-FLI1 Function in Ewing Sarcoma
… CDK8/19 in EWS-FLI1 transcriptional repression. Chromatin profiling of CDK8 and EWS-FLI1 shows colocalization across the chromatin. In addition, treatment of Ewing sarcoma cells with either known CDK8/19 inhibitors or our novel CDK8/19 inhibitor causes growth inhibition.
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Investigating DNA G-quadruplex structure function through genome and epigenome engineering
… of the *MYC* oncogene. In combination with chromatin profiling, whole-genome and locus-specific sequencing, and biophysical assays, I investigated how G4 folding in cells regulates the local chromatin environment and gene expression. I found that the *MYC* G4 structure positively regulates …
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Genomic architecture of the aging hematopoietic system
… cells, together with whole genome sequencing and chromatin profiling on bulk CD45+ cells to reveal genetic, epigenetic, and transcriptional factors underlying healthy immune aging. I show that sex and cell-type specific transcriptional signatures rather than cell composition differentiate …
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Characterising the gene regulatory landscape of CD4+ T cells
… followed by genome-wide transcriptome and chromatin profiling of naive and memory cells. I observed that CD28 plays a critical role in the expression of genes involved in effector functions, cell cycle regulation in memory T cells and in disease susceptibility. I profiled the gene …
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Not Black and White: BMP Signaling Drives Melanocyte Differentiation Downstream of Stem Cell Activation
… mutant mouse models, cell culture systems, and chromatin profiling, I provide evidence demonstrating cooperation of BMP and WNT signaling to trigger complete differentiation of McCP into melanocytes through MITF and LEF1 activity. Altogether, I have generated a thorough characterization of the …
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Home Sweet Home: Epigenetic Paths of Stem Cells In and Out of Their Niche
… are well-characterized. By mapping global chromatin domains of HFSCs in their native niche, and of committed progenitors, I discovered that super-enhancers and their dense clusters ('epicenters') of transcription factor binding sites change dramatically upon lineage progression. New fate is …