Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"Chromatin Organization"”.
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Investigating the role of molecular motors on chromatin organization
In eukaryotes, chromatin carrying most of the genetic material and information is densely packed within the cell nucleus. To facilitate the proper functioning of numerous life-essential biological processes, it possesses multiple levels of packaging. On one hand, nucleosomes, as the most …
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Understanding Chromatin Organization and Dynamics with Coarse-Grained Modeling
… human life, and it is crucial to understand its organization. The genome organization is hierarchical with different principles dominating at different scales. At the near-atomistic level, nucleosomes are organized as ordered chromatin fibers or disordered chromatin arrays. Furthermore, chromatin …
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Changes of nucleosome positioning and 3D chromatin organization in cell transitions
… the nucleus in a highly condensed form called chromatin. The basic unit of chromatin is the nucleosome. The positioning of nucleosomes on the DNA determines the accessibility of transcription factors (TFs) and other regulatory molecules. Beyond nucleosome positioning, the higher level of 3D …
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Role of ORCA and ORC in chromatin organization and DNA replication
… or individual subunits of ORC including heterochromatin organization, telomere and centromere function, centrosome duplication and cytokinesis. Heterochromatic domains are enriched with repressive histone marks, including histone H3 lysine 9 methylation, written by lysine methyltransferases …
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Biomechanical regulation of epigenetics and chromatin organization in single living cells
… mechanisms include epigenetic modification, chromatin organization, and gene expression. It is, therefore, highly important to investigate force-induced regulation of these mechanisms to better understand human physiology and disease. In this research, we aim at answering two key questions: …
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Dissecting The Roles of Human Smc Complexes In Transcription Regulation and Chromatin Organization
… and therefore they have to forge highly complex chromatin loops to spatially bridge these regulatory elements and genes in the three-dimensional (3D) genome. However, the hierarchy of chromatin contacts and their underlying mechanisms are not well-understood. SMC complexes including Cohesin …
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Creating a versatile toolkit for transgene expression using BACS and for dissecting large-scale chromatin organization
… highly compacted with histone proteins to form chromatin in interphase nuclei. Both the higher levels of chromatin folding and the spatial organization of the chromatin, referred to as large-scale chromatin organization, have been shown to correlate with transcriptional activity. One example …
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Chromatin organization as a possible factor in the control of susceptibility to radiation-induced AML in mice
To view the abstract, please see the full text of the document.
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Evolution of nucleosome positioning and gene regulation in yeasts : a genomic and computational approach
Chromatin organization plays a major role in gene regulation and can affect the function and evolution of new transcriptional programs. Here, we present the first multi-species comparative genomic analysis of the relationship between chromatin organization and gene expression by measuring mRNA …
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UNDERSTANDING LAMIN A/C AND ITS ROLES IN DISEASE PATHOLOGIES
… COMPRISED OF THE A AND B-TYPE LAMINS, REGULATES CHROMATIN ORGANIZATION AND GENE TRANSCRIPTION. MOUSE MODELS WERE DERIVED TO STUDY THE ROLE OF THE A-TYPE LAMINS IN INTESTINES AND SKIN HOMEOSTASIS AND ONCOGENESIS. MICE LACKING LMNA IN THEIR GASTROINTESTINAL (GI) EPITHELIUM HAVE NO EFFECTS ON …
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The OCA2-HERC2 Locus: Polymorphisms and Eye Color Determination, Chromatin Organization in the Interphase Nucleus, and Forensic Biotechnology Applications [I geni HERC2 e OCA2: polimorfismi e colorazione degli occhi, organizzazione nel nucleo interfasico e applicazioni biotecnologiche in ambito forense]
La Fenotipizzazione del DNA Forense (FDP) ha migliorato le indagini forensi, consentendo la predizione di tratti fenotipici come il colore degli occhi, influenzato dal locus OCA2-HERC2 e dallo SNP rs12913832. Sebbene efficace per colori chiari e scuri, il metodo mostra limiti nel predire tonalità …
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Deriving Structural Insights About Mesoscale Chromatin Folding Using Coarse-Grained Oligonucleosome Modeling
<p>Here, I investigate chromatin organization on the scale of a few nucleosomes (~1kb). I developed an experimentally-validated coarse-grained polymer model and applied it 1) to show how chromatin fiber parameters, such as nucleosome spacing and breathing, impact chromatin organization on this …
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Transcriptional and Chromatin Disorientation Associated with BRCA2 Inactivation
… via regulating the transcriptional and chromatin organization. Using RNA seq datasets from BRCA2 mutant MCF10A cell lines and TCGA breast cancers, I report that BRCA2 inactivation leads to dysregulation of genes involved in the cell cycle, DNA repair, and replication. Strikingly, …
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Genomic and microscopic dissection of large-scale chromatin compaction
Large-scale chromatin compaction varies across the human genome, and these variations correlate with differences in transcriptional activity in a limited number of model systems. However, without validated genome-wide methods directly measuring large-scale chromatin compaction, the degree to which …
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Characterizing the Role of SUMO in Transcriptional Repression in S. cerevisiae
… implicated in transcriptional regulation and chromatin organization, although the nature of its role remains unclear. This thesis aims to: 1) complete a comprehensive characterization of the SSR, and 2) elucidate the true role of SUMO in transcriptional regulation and chromatin organization. …
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Interphase chromosome movement during the midblastula transition in Drosophila melanogaster
Eukaryotic chromatin is functionally active only in the interphase nucleus. Indirectly we know that global chromatin changes occur, such that gene expression and replication proceed. I undertook to directly observe the structural changes of interphase chromatin, at a time in Drosophila …
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Pressure Treatment of Polytene Chromosomes and Computer Vision Techniques for High Resolution Cytogenetic Studies
… exceptional cytology provided by the interphase chromatin organization in polytene chromosomes has made Drosophila the premier model organism for analysis of chromosome structure and behavior. A major impediment to full exploitation of polytene chromosome cytology has been the difficulty in …
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Identification and Characterization of Factors Involved in Sodium Selenite Toxicity
… interact with Topoisomerase 2 (Top2) to organize chromatin organization, I further elaborated the role of Top2 in SeL mediated DNA damage formation. Notably, my results suggest that SeL-dependent chromosome fragmentation is enhanced in cells lacking Top2 activity. I further characterized factors …
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GATA1 Occupancy Site Regulates Erythroid Cell Fitness through Pleiotropic Functions
… about regulatory sequence grammar, complex chromatin environments, and the transcriptional regulatory networks that link genotype to phenotype. In this study, we combined base editor-mediated perturbations of regulatory elements, CRISPR-mediated gene disruptions, epigenetic profiling, and …
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Native chromatin extraction and its structure analysis
Structure determines function. Properly ordered chromatin organization is fundamental for accurate regulation of gene expression inside eukaryotes. A native chromatin extraction method is developed based on solid phase reversible immobilization (SPRI) using magnetic nanoparticles as carriers. This …
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