Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Chromatin Immunoprecipitation Sequencing (ChIP-Seq)"”.
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A High-Throughput Approach to Uncover Novel Roles of APOBEC2, a Functional Orphan of the AID/APOBEC Family
… For this thesis, I have combined Next Generation Sequencing (NGS) techniques with state-of-the-art molecular biology to determine the physiological targets of APOBEC2. Using a cell culture muscle differentiation system, and RNA sequencing (RNA-Seq) by polyA capture, I demonstrated that unlike the …
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Analysis of ASCL1 in Neuroendocrine Lung Cancer
… targets of ASCL1, whole-genome RNA-sequencing (RNA-seq) analysis combined with chromatin immunoprecipitation-sequencing (ChIP-seq) were performed with a series of lung cancer cell lines. We discovered the gene SCNN1A, which encodes the alpha subunit of the epithelial sodium channel …
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The Expression of the Zinc Finger Transcription Factor zDC Defines the Classical Dendritic Cell Lineage
… genes occupied and regulated by zDC, I performed chromatin immunoprecipitation-sequencing (ChIP-seq) on zDC from steady state cDCs. zDC bound upstream of the transcription start sites of over 1,000 genes at sequence-specific motifs. (3) To interrogate zDC function, I generated zDC-deficient mice …
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Characterizing the transcriptional regulation of crassulacean acid metabolism in Kalanchoe
… and KfMYB59 were selected for chromatin immunoprecipitation-sequencing (ChIP-seq). However, these experiments failed to identify enriched target genomic loci possibly as a consequence of the unique challenges of adapting experimental protocols designed for model C3 …
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Identification and characterization of factors involved in DNA demethylation and anti-silencing in Arabidopsis
… repressors and protein factors for heterochromatin formation. Our data show that Arabidopsis MBD7 functioins as a cellular anti-silencing factor. MBD7 is required for the expression of transgenes by preventing DNA hypermethylation at the transgene promoter. Analysis of the methylome …
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DIABETES IMPAIRS THERAPEUTIC EFFECT OF ENDOTHELIAL PROGENITOR CELL EXOSOME-MEDIATED MYOCARDIAL REPAIR
… endothelial cell gene expression. The H3K9Ac chromatin immunoprecipitation sequencing (ChIP-Seq) results further revealed that diabetic EPC-Exo reduced H3K9Ac level on angiogenic, cell survival, and proliferative genes in MCECs. Moreover, we found that a small molecular inhibitor of HDACs, …
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Global transcriptional, post-transcriptional, and translational regulation during soybean seed and seedling development
… gene regulatory networks, high-throughput RNA sequencing (RNA-Seq) was carried out using cotyledons from seven developmental stages of soybean seedlings. A total of 154 genes demonstrated higher level of expression exclusively in the early seedling stages. About 25% of those genes with known …