Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 1351 for “"Chromatin"”.
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From Technology to Chromatin Biology: Contemporary Mass Spectrometry of Histones and Chromatin Associated Proteins
… of top down and middle down MS to other chromatin-associated proteins like HMGA1. In this study we were able to link PTM dynamics (phosphorylation of Ser 43) on HMGA1a to DNA repair and the early events in apoptosis.
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From Technology to Chromatin Biology: Contemporary Mass Spectrometry of Histones and Chromatin Associated Proteins
Made available in DSpace on 2014-12-17T21:58:34Z (GMT). No. of bitstreams: 1 3347511.pdf: 6286893 bytes, checksum: a541f3eac3d2d43e52f4e03c2218cf78 (MD5) Previous issue date: 2008
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Chromatin Accessibility Beyond the Peaks
Chromatin state resides at the intersection of trans-acting factors that operate globally over the genome but respond to changing conditions, and DNA sequence which is invariant across conditions but varies locally around genes. Assays that measure features of chromatin state can help us understand …
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Chromatin Dynamics During DNA Replication
The primary level of chromatin organisation consists of arrays of nucleosomes that are present across the genetic template. Advances in the post genomics era have made it possible to determine the positions of nucleosomes genome-wide where it has been observed that nucleosomes adopt a distinct …
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Apoptosis-induced chromatin modifications in lupus.
Contains fulltext : 82617.pdf (Publisher’s version ) (Open Access)
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Analysis of Human Sperm Chromatin Integrity
… material in the two condensation stages of chromatin and chromosomes. Original semen samples (ORI) and their swim-up fractions (SW, selected for motility) from men of known (donors) and unknown (patients) fertility were analyzed. The extent of chromatin condensation was assessed by light …
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Hypochlorous Acid Mediated Damage of Chromatin
<p>Hypochlorous acid (HOCl), the primary product of activated neutrophils at sites of inflammation, can damage both DNA and associated histone proteins. HOCl damage to cytosine bases in DNA generates the chlorination damage products 5-chlorocytosine (ClC) and 5-chlorouracil (ClU) as well as the …
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Exploiting Lentiviral Integrations to Profile Chromatin
… modifications and protein localisation along chromatin suffer from critical limitations: the gold standard, ChIP-seq, relies on high-quality antibodies, while other antibody-free techniques like DamID are limited by the availability of specific DNA sequences. Here, I describe the development …
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Compaction of chromatin in Saccharomyces cerevisiae
… phase. The relative gene content of condensed chromatin, fractionated and isolated by sucrose gradient ultracentrifugation from stationary and exponential phase cultures was compared using genome-wide technologies. This study showed that condensed chromatin of stationary phase culture contained …
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3D chromosome structure and chromatin proteomics
… part focuses on developing an approach called "chromatin proteomic profiling" to identify protein factors associated with various active and repressed portions of the genome marked by specific histone modifications. The histone modifications assayed by chromatin proteomic profiling are …
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Dissecting the Architectural Properties of Chromatin and the Influence of the H1 Linker Histone on Chromatin Regulation
… this research, I used an existing coarse-grained chromatin model from the Collepardo Lab to study the binding of the H1 linker histone to chromatin and delve into the intrinsic properties of chromatin that regulate its architecture. The primary aim is to decipher the factors modulating chromatin …
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Roles of H2A.z in Fission Yeast Chromatin
… variants are shown to coincide with different chromatin compartments and mark active or repressed genes. Msc1 is one of the seven JmjC Domain Proteins (JDPs) in Fission Yeast. JDPs are known to function in chromatin and some act as histone demethylases. We found that Msc1 is a member of Swr1 …
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Native chromatin extraction and its structure analysis
Structure determines function. Properly ordered chromatin organization is fundamental for accurate regulation of gene expression inside eukaryotes. A native chromatin extraction method is developed based on solid phase reversible immobilization (SPRI) using magnetic nanoparticles as carriers. This …
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Sculpting 3d Chromatin Folding Via Genome Editing
… and initiated contacts reflective of chromatin loop extrusion. Deletions of the CBS or the TSS individually or in combination within inserts revealed their distinct contributions to genome folding. Altogether, short DNA insertions can suffice to shape the spatial genome in a manner …
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UNCOVERING STRUCTURE-FUNCTION RELATIONSHIPS IN CHROMATIN ARCHITECTURE
… enabled the creation of high-resolution maps of chromatin architecture genome-wide from which to test new biological hypotheses. In this thesis, we first present a networks based algorithm, 3DNetMod, for identifying the prominent feature of Topological Associating Domains (TADs) and their inner …
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In silicio Streckung und Kompaktierung von Chromatin
… die Streckung und Kompaktierung einzelner 30-nm-Chromatinfibern mit Hilfe von Monte-Carlo-Simulationen untersucht. Die DNS wird als flexible Polymerkette mit einer Debye-Hückel-Elektrostatik approximiert und basiert auf dem „Zwei-Winkel-Modell“. Nukleosomen werden durch oblate Ellipsoide …
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Dynamic Simulation of active/inactive Chromatin Domains
… which describes the formation of higher order chromatin structures with the help of polymer physics for the first time. It is a block-copolymer model for the compactification of the 30nm Chromatin fiber into higher order structures. The idea is that basically every condensing agent (HMG/SAR, …
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Investigating the chromatin dynamics of gene activation
… previously unknown. I investigated changes in chromatin accessibility and modifications to show that Shh expression is activated from endodermal enhancers when mESCs are differentiated with retinoic acid. RNA-seq confirmed that the resulting cells express several markers of early endoderm and …
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Structural Investigation of the Chromatin Remodeller Fun30
Manipulation of chromatin structure is closely linked to the process of gene regulation. The core component of chromatin, the nucleosome, consists of approximately 147 base pairs of DNA wrapped around a protein core consisting of two histone H2A-H2B dimers and a histone H3-H4 tetramer. Chromatin …
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