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Showing 1 to 13 of 13 for “"Chondroitinase ABC"”.
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Understanding the catalytic machinery of chondroitinase ABC I in processing dermatan sulfate
… serve as inhibitors to neuron regeneration. The chondroitinase enzyme family is comprised of several bacterial lyases known to dissolve galactosaminoglycans in the extracellular matrix. Although several studies have shown the benefit of using chondroitinase enzymes for treatment, there is much to …
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Characterising changes in pathology and function in clinically relevant models of spinal cord injury and using chondroitinase ABC gene therapy to promote repair
Over recent years the bacterial enzyme chondroitinase ABC (ChABC) has emerged as a promising experimental therapeutic for the treatment of spinal cord injury (SCI). In pre-clinical studies ChABC has repeatedly been shown to enhance functional recovery in a number of SCI models in both rodents and …
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Analysis of the Structural Units of Sulfated Glycosaminoglycans by High Performance Ion Exchange Chromatography
… be structurally characterized in terms of their chondroitinase ABC digestion products. The high performance ion exchange chromatography technique has been used in the analysis of the oligosaccharide composition of a series of heparins fractionated by Dowex anion exchange chromatography and in the …
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Structural and Metabolic Studies of Glycosaminoglycans (Sulfatase, Hydrazine, Mucopolysaccharidosis)
… released by treatment of these polymers with chondroitinase ABC or chondroitinase AC. A study of the rates of disaccharide release during the N(,2)H(,4)/HONO treatment of reference GAGs indicated that 4-sulfation of CS significantly decreased the overall N-deacetylation rate.
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Impact loading and functional tissue engineering of articular cartilage
… of established disease, the GAG degrading enzyme chondroitinase ABC (C-ABC) improved the tensile integrity of articular cartilage constructs grown with a scaffold-less approach. C-ABC application increased ultimate tensile strength and tensile stiffness, reaching values of 1.4 and 3.4 MPa, …
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Tissue-Engineered Annulus Fibrosus Repair and Nucleus Pulposus Augmentation in Pre-Clinical Models of Intervertebral Disc Degeneration
… and quality of AF repair in vivo (Chapter 4). Chondroitinase ABC was investigated ex vivo as a pretreatment to improve adhesion of the AF repair strategy to native tissue (Chapter 5). Lastly, Fourier transform infrared microscopy was utilized to quantitatively analyze the local biochemical …
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Tools for decoding the structure-function relationships of biopolymers in nanotechnology and glycobiology
… biochemical-structural characterization of the Chondroitinase ABC-II enzyme that depolymerizes dermatan sulfate and chondroitin sulfate GAGs (CSGAGs), and the 6-0- Sulfatase and N-Sulfamidase enzymes that de-sulfate functional groups on heparin and heparan sulfate GAGs (HSGAGs) are described. …
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Collagen scaffolds in full- and hemi- resection spinal cord injury models
… delivering either soluble Nogo receptor (sNgR), chondroitinase ABC (ChABC), or bone marrow derived mesenchymal stem cells (MSCs). Improvement in hindlimb function over four weeks following injury was seen in the DHT scaffold, EDAC scaffold + ChABC, and EDAC scaffold + MSCs groups, but not in the …
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Poly(N-Isopropylacrylamide)-based scaffolds for tissue engineering applications
… that is negatively charged and degraded by chondroitinase ABC enzymes. Incorporating CS into PNIPAAm will create a three-dimensional hydrated copolymer network with increased elasticity and biocompatibility. As before, the thermoresponsive properties of PNIPAAm enable the copolymer to be …
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Purification and Characterization of Proteoglycan from Bovine Aortic Endothelial Cells Conditioned Media, and its Interaction with Basic Fibroblast Growth Factor (bFGF)
… of chondroitin sulfate, which can be digested by Chondroitinase ABC. Gel filtration chromatography (Sepharose CL-2B and CL-4B columns) showed that BAE PG consisted of two different sized peaks, and had an average molecular weight of approximately 5 x 10⁵ Da. SDS-PAGE with silver staining indicated …
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Studies of plasticity after neuronal injury
… to promote plastic changes, that is, the enzyme chondroitinase ABC (ChABC) which degrades chondroitin sulphate proteoglycans - a major class of inhibitory extracellular matrix molecules. Viral vector technology was used to optimise the delivery of ChABC by gene transfer to host spinal cord cells. …
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Molecular studies of aggrecan : experiments and simulations
… (GAGs) via enzymes Keratanase II (K'ase) and Chondroitinase ABC (C'ase) respectively. The aggrecan was extracted and purified from the intervertebral disc of a 24-year old human (obtained from the Shriner's Hospital, Montreal) and separated into two pools: the HA-associated pool is composed of …