Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Chloride Channels"”.
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Isolation and partial characterization of three acetylcholine-gated chloride channels in Haemonchus contortus
… The Cys-loop superfamily of ligand gated ion channels are well recognized as critical drug targets for many invertebrate specific compounds. With the rise in resistance seen worldwide to existing anthelmintics, novel drug targets must be identified so new treatments can be developed. This …
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Chloride Channels in Normal Human Blood Monocytes and a Macrophage-like Cell Line
… and volume change are unclear.|Alteration in ion channels, especially chloride channels, is generally involved in the shape and volume change of circulating cells for successful invasion into tissues. In this work, we examined chloride currents in human blood monocytes and macrophages, and their …
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HUMAN CLCA2 MODULATES THE CONDUCTANCE OF CALCIUM-ACTIVATED CHLORIDE CHANNELS BY REGULATION OF INTRACELLULAR CALCIUM
Chloride channels play an essential role in the physiology of the respiratory system, the gastrointestinal tract, and secretory glands. Their dysregulation underlies debilitating pathologies such as cystic fibrosis, asthma, and certain cancers. The CLCA (Chloride Channel Accessory) gene family is …
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Structure-Function Studies on Human Epithelial Cystic Fibrosis Chloride Channels Expressed in Xenopus Laevis Oocytes
… originally ascribed to the R domain. Here, CFTR channels were severed near likely N- or C-terminal boundaries of NBD1 or the R domain. Channel activity, assayed via two-microelectrode voltage clamp monitored successful assembly of pairs of channel segments as the sever point was systematically …
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Identification and characterization novel of cystine-loop ligand- gated chloride channels from Dirofilaria immitis: pharmacological analysis and novel compound screening
… an alarming rate. Cystine-loop ligand-gated ion channels are important receptors in nematode neurobiology, and as such are promising drug targets. The UNC-49 (GABA-gated chloride channel) and ACC (acetylcholine-gated chloride channel) family of receptors have been characterized as potential drug …
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Are Post-Transcriptional RNA Modification Events in the Glutamate-Gated and GABA-Gated Chloride Channels of Anopheles minimus and Anopheles dirus associated with Ivermectin Insensitivity?
… glutamate-gated (GluCl) and the GABA-gated (RDL) chloride channels. The mechanism by which this is achieved is poorly understood. Using a combination of molecular and bioinformatic techniques such as DNA/RNA extraction, PCR amplification and DNA/RNA sequence analysis of the GluCl and RDL channels, …
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Exploiting muscarinic acetylcholine receptors as an insecticidal target to enhance the toxicity of gamma-amino butyric acid channel blockers and the continued challenges with resistance
… targeting gamma-aminobutyric acid (GABA)-gated chloride channels, indicating the potential of insect mAChRs as a target for developing novel insecticides/synergists to control resistant pests. A point mutation (A301S) in the GABA-gated chloride channel confers resistance to dieldrin (Rdl), …
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Macromolecular Complexes of Cystic Fibrosis Transmembrane Conductance Regulator Alter Fluid Transport in inflammatory Bowel Disorders
… that can regulate cAMP-/cGMP-activated CFTR chloride channel function. We report the physical and functional coupling of CFTR with nitric oxide (NO) producing enzyme-inducible nitric oxide synthase (iNOS) at the apical plasma membrane in inflammatory bowel disease (IBD). The complex formation …
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Investigation into the role of binding loop E on the function of the nematode cys-loop GABA receptor.
… novel drug targets such as ligand-gated chloride channels (LGCCs) need to be characterized. The objective of this thesis was to further characterize the agonist binding pocket in Hco-UNC-49BC, the LGCC gated by γ- aminobutyric acid (GABA) within H. contortus. To meet this objective, each …
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Computational studies of membrane proteins: Structure, function and dynamics
Ion channels are membrane proteins that regulate the flow of ions across the cell membrane. These channels open in response to changes in electrostatic potential across the cell membrane or binding of small molecules to the channels and allow the passage of specific ions through their conduction …
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STRUCTURE AND FUNCTION OF A NEURONAL GLUTAMATE TRANSPORTER
… of glutamate, the transporters also mediate a chloride conductance that is gated by Na+ and glutamate. Our data indicate that glutamate binding sites, transport pathways, and chloride channels reside in individual subunits in the trimer and function independently and that the anion channel is …
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STRUCTURE AND FUNCTION OF A NEURONAL GLUTAMATE TRANSPORTER
… of glutamate, the transporters also mediate a chloride conductance that is gated by Na+ and glutamate. Our data indicate that glutamate binding sites, transport pathways, and chloride channels reside in individual subunits in the trimer and function independently and that the anion channel is …
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Study of correlation analysis for protein-protein interactions
… applied this method onto epithelial sodium and chloride channels, which included CFTR and ENaC, Clc2 and ENaC, CACC and CFTR, trying to identify the possible networks of sodium and chloride channels in the airway epithelia. This provided us the possible interactions of Clc2 and ENaC γ subunit, …
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Toxicological Analysis of Acaricides for Varroa Mite Management
… and diseases in bee colonies. Voltage-gated chloride channels are involved in the maintenance of nerve and muscle excitability in arthropod pests, which suggests that these channels might be exploited as targets for acaricides. Apistan® (the pyrethroid tau-fluvalinate), Checkmite+® (the …
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Characterization of the ACC-1 Family of cys-loop ligand gated ion channels from the parasitic nematode Haemonchus contortus.
Nematode cys-loop ligand-gated ion channels (LGICs) have been extensively studied for decades because of their role in current anthelmintic action and potential as targets for future drugs. Many families of cys loop receptors have not yet been pharmacologically characterized in parasitic nematodes, …
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Design, Synthesis and Characterization of Synthetic Ion Receptors as Biologically Functional Ion Carriers and Channels
… transporters as well as natural ion carriers and channels. Among the studies that are performed, is the design, synthesis and characterization of members of the dipicolinic dianilide class of compounds as synthetic chloride channels. In originating this project, an array of both known and novel …
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Nongenomic Effects of Estrogens on Epithelial Chloride Secretion.
… cell line T84, a model for studying epithelial chloride secretion and cystic fibrosis chloride channel (CFTR) function, was used to investigate the regulatory role of estrogens in transepithelial ion transport. Estrogens and other steroid hormones do not stimulate chloride secretion by …
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Chloride Channel 3 (CLC3), an Immune Antiporter in the Migration and Activation of Human Eosinophils in Allergic Asthma
… has also been implicated in the modulation of chloride channels including CLC3, which is required for the respiratory burst by eosinophils and neutrophils, and altering the shape to cause migration of neutrophils. However, the underlying mechanism of eosinophil migration and activation is not …
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TGF-β1-induced Chloride Channel Activity and Migration of Human Eosinophils
… airway remodeling. TGF-β activity also affects chloride channel activity, which can regulate mediator release and cell migration. Therefore,the role that TGF-β1 stimulation of eosinophils has on chloride channel activity and migration of eosinophils and the underlying mechanisms involved in this …
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The Cystic Fibrosis Transmembrane Conductance Regulator: Regulation by HSP-90
… measured from Xenopus oocytes expressing CFTR chloride channels. When assessing the phosphorylation status of CFTR, it is seen that under conditions that increase the association of Hsp90, there is also observed an increase in the phosphorylation of CFTR. Conversely, in the presence of …
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