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Showing 1 to 20 of 105 for “"Chlamydomonas reinhardtii"”.
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Eyespot Assembly and Positioning in Chlamydomonas reinhardtii
… of the biflagellate unicellular green alga Chlamydomonas reinhardtii is a complex organelle that facilitates directional responses of the cell to environmental light stimuli. The eyespot, which assembles de novo after every cell division and retains a distinctive association with the …
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Cell Cycle Control by Cyclin-CDKS in Chlamydomonas Reinhardtii
… lineage of particular concern to humanity. Chlamydomonas reinhardtii is a unicellular member of the plant kingdom. Its simple genome (compared to land plants) and easily observed cell division cycle have facilitated the collection of a large number of conditional mutations that block the …
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Nuclear proteomics and transcription factor profiling in Chlamydomonas reinhardtii
… processes is the unicellular green algae Chlamydomonas reinhardtii, due to advantages related to culturing, genetic manipulation and availability of genome sequence. In the present study, we were interested in understanding the regulatory mechanisms underlying photosynthesis-related …
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CHLAMY 1, ein circadianes RNS-Bindeprotein aus Chlamydomonas reinhardtii
… des RNS-Bindeproteins CHLAMY 1 aus C. reinhardtii wurden unter Verwendung eines chimären Reporterkonstruktes überprüft. Hierdurch konnte gezeigt werden, dass ein in CHLAMY 1- angereichertes Proteingemisch die Translation dieses Reporterkonstruktes in einem zellfreien System um …
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Photosynthetic hydrogen production from the green alga Chlamydomonas reinhardtii
Includes bibliographical references (pages 119-134).
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Regulation of the carbon-concentrating mechanism in Chlamydomonas reinhardtii
… environments. In the model eukaryotic green alga Chlamydomonas reinhardtii, the CCM is induced under low CO2 in the light and comprises: active inorganic carbon transport systems, carbonic anhydrases and the localisation of Rubisco to a central chloroplast microcompartment called the pyrenoid. In …
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Physiological and molecular determinants of the Chlamydomonas reinhardtii pyrenoid
… associated CCM, was studied using the model alga Chlamydomonas reinhardtii. The starting premise was the report by Nozaki et al. (2002) that, in some species of the family Chlamydomonaceae, a few amino acid residues within the RuBisCO large subunit (LSU) correlated strongly with pyrenoid …
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Small RNA and genome interactions in Chlamydomonas reinhardtii recombinants
… phenotype, I designed crossing experiments with Chlamydomonas reinhardtii, choosing this unicellular alga due to the genetic tools available and the haploid nature of its vegetative cells. The specific aim of the experiment was to identify transgressively expressed sRNA populations. Crossing two …
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Photoprotection and chloroplast regulation in the green algae Chlamydomonas reinhardtii
… light regimes in the model green algae Chlamydomonas reinhardtii, to characterize potential energy dissipating mechanisms that may occur in absence of qE and identify factors regulating the expression of the chloroplast encoded photosystem I subunit, psaA, using a forward genetic screen. …
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Genome Scale Metabolic Modelling of Arabidopsis thaliana and Chlamydomonas reinhardtii
… in plants and algae, GSMs of A. thaliana and C. reinhardtii have been constructed using annotations from their respective BioCyc databases. They satisfy all theoretical considerations and are able to represent known biological behaviors and thus can be used in subsequent investigations. In …
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Molecular characterization of phosphoribulokinase and rubisco activase from Chlamydomonas reinhardtii
Chlamydomonas reinhardtii phosphoribulokinase was purified to apparent homogeneity and polyclonal antibodies were prepared. Immunoblot analysis indicated antigenic similarity and a similar subunit size for the enzyme from five higher plant species and Chlamydomonas. Immunoblots also revealed …
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Investigating the molecular basis of vitamin B12 uptake in Chlamydomonas reinhardtii
… green algae. In this study, the model green alga Chlamydomonas reinhardtii was used to investigate B12 uptake. Candidate genes related to B12 transport and binding were identified in C. reinhardtii from sequence similarity to genes known to bind or transport B12 in other organisms. Six genes were …
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Secondary Level Screening of Chlamydomonas Reinhardtii Mutants Defective in Circadian Gene Expression
… control, this work focuses on the isolation of Chlamydomonas reinhardtii mutants which are defective in circadian gene expression. In a previous study, the reporter gene ARS2 encoding the arylsulfatase enzyme was fused to the promoter of the circadian-regulated CABII-1 gene and transformed into …
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Morphological and Physiological Studies of the Carbon Concentrating Mechanism in Chlamydomonas reinhardtii
Chlamydomonas reinhardtii possesses a single-cell-based CO2-concentrating mechanism (CCM). The CCM is an important element of algal photosynthesis, metabolism, growth and biomass production, which works by increasing the concentration of inorganic carbon (Ci) in the pyrenoid, a dense RuBisCO-packed …
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Toxicity, morphological changes, and dissipation of oil sands naphthenic acids in Chlamydomonas reinhardtii
… effects of NAFCs on the unicellular green algae Chlamydomonas reinhardtii wild type (WT) and two cell wall mutants: CC-400, which retains the innermost and outermost glycoprotein wall layers, and CC-3395, believed to be completely naked. The presence of the cell wall was strongly linked to NAFC …
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Isolation and characterization of two closely related beta-carbonic anhydrases of Chlamydomonas reinhardtii
… organisms such as the green alga, C. reinhardtii respond to low carbon dioxide conditions by inducing a carbon dioxide concentrating mechanism (CCM). Important components of the CCM are carbonic anhydrases (CAs), zinc metalloenzymes that catalyze the interconversion of carbon dioxide …
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Photochemically produced reactive species generation by extracellular organic matter sensitizers from Chlamydomonas reinhardtii
… solutions separated from pure batch cultures of Chlamydomonas reinhardtii under solar irradiation. Results show increasing steady-state levels of 3DOM, 1O2, and formation rates of •OH under sunlight irradiation as EOM levels increase with batch culture growth. Changes in reactive species levels …
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Evaluation of the effects of dye-mediated irradiation on metabolite production in Chlamydomonas reinhardtii
Submitted in fulfilment of the requirements of the degree of Doctor of Philosophy of Applied Science in Biotechnology, Durban University of Technology, 2022.
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