Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Chinese hamster ovary cells"”.
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Micro-bioreactor design for Chinese hamster ovary cells
… to design a micro-bioreactor for the culture of Chinese Hamster Ovary (CHO) cells. There is an increasing demand for upstream development in high-throughput micro-bioreactors specifically for the recombinant CHO cell line, an important cell line for producing recombinant protein therapeutics. In …
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Genomic Analysis And Engineering Of Chinese Hamster Ovary Cells For Improved Therapeutic Protein Production
… These complex molecules are produced in living cells, primarily Chinese Hamster Ovary (CHO) cells. Due to the importance of these therapeutic proteins to disease treatment, it is essential to improve the efficiency of their production, both to promote the development of new therapies, and to …
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Studies on the internalization and intracellular transport of horseradish peroxidase in Chinese hamster ovary cells
… horseradish peroxidase (HRP) is internalized by Chinese hamster ovary cells, a cell line of fibroblastic origin (Adams et al., 1982). We have confirmed this result by showing no inhibition of uptake in the presence of divalent cation chelators (EGTA Mg or EDTA), excess (19 mg/ml) yeast mannan (an …
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Identification and characterisation of a novel gene, DWNN, isolated from promoter-trapped Chinese hamster ovary cells
… destruction of foreign antigens by cytotoxic T cells and is of crucial importance to the defence of the organism against viral infections. Defects in this process can lead to various autoimmune diseases and cancer. The aim of this study was to identify more genes involved in the cell death …
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The Engineering of Chinese Hamster Ovary Cells to Achieve More Efficient Gene Amplification for Improving Biopharmaceutical Development
… issue of the long development times of obtaining Chinese hamster ovary (CHO) cells capable of producing high quantities of therapeutic proteins. It addresses the specific time bottlenecks associated with developing high producing CHO cell lines and reviews various methods that are employed to …
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The human cannabinoid CB(1) receptor stably expressed in Chinese hamster ovary cells provides a model system to predict the pharmacological effects of cannabinoids in man
… CB₁ receptor has been stably expressed in Chinese Hamster Ovary (CHO/CB₁) cells, which do not endogenously express and known cannabinoid receptors. Saturation binding studies using [³H]SR141716A and [³H]CP55,940 determined that this system expresses the human cannabinoid CB₁ receptor at …
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Analysis of the roles of ligand and DNA binding in estrogen receptor action
… is unable to activate transcription. Studies of Chinese Hamster Ovary cells which were grown under serum-free conditions, suggested that commercial base media may contain traces of estrogenic compounds. Additional studies demonstrated that transcriptional synergy by ER is strongly dependent on …
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Hv1 novel peptide inhibitor extracted from tarantula venom
… patch clamp techniques on genetically modified Chinese Hamster Ovary cells expressing human Hv1, the study revealed that GsAF-I significantly stabilizes the closed state of these channels, thereby delaying their opening and hastening their closure, with these effects being notably …
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Comparison of the growth and monoclonal antibody production of suspended mammalian cells in three perfusion systems
… and monoclonal antibody production by HPCHO Chinese hamster ovary cells and IB4 hybridoma cells in batch, fed-batch, and three perfusion systems: the stirred ceramic membrane reactor (SCMR), the alternating tangential flow (ATF) hollow fiber membrane system, and the external spin filter (ESF) …
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Effect of continuous irradiation on proliferation of CHO-K1 and xrs-6 cells under low and high oxygen conditions
… 1) the effect of continuous irradiation on Chinese Hamster Ovary cells, CHO-K1 (wild type) and xrs-6 cells (mutant), and 2) the effect of continuous irradiation under low (5%) and high (20%) ambient oxygen conditions. Other authors have found continuous irradiation at low dose rates to have …
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Overexpression of Amyloid Precursor Protein Induces Mitochondrial Oxidative Stress and Activates the Intrinsic Apoptosis Pathway
… induced by overexpression of wild type APP. Chinese hamster ovary cells transfected with a DsRed-APP fusion construct displayed caspase-3 activation and nuclear fragmentation indicative of apoptosis. APP-induced apoptosis was blocked by a pan-caspase inhibitor, (BOC), glutathione (GSH), or …
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Studies on the biosynthesis of lipoic acid
… over the next five hours of aerated cell growth. Chinese hamster ovary cells were found not to incorporate the two known bacterial precursors, [²H₃]-acetate and [U-²H₁₅]-octanoate, into lipoic acid. Further studies with both Chinese hamster ovary and mouse fibroblast cells which were designed to …
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Case studies in the modeling and control of continuous pharmaceutical manufacturing processes
… or produced via recombinant hosts such as Chinese hamster ovary (CHO), Escherichia coli, or Saccharomyces cerevisiae, with some products requiring both a recombinant host and transient transfection or infection with additional genetic material. Across these modalities, industry, regulatory …
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von Willebrand factor interaction with subendothelial collagens and platelet surface receptor GPIBalpha under shear conditions
… vWf binding to glycocalicin-coated beads, Chinese hamster ovary cells expressing the wild type and mutant GPIb-IX complex, and washed human platelets. Compared to binding in the presence of botrocetin and ristocetin, shear stress induces only low-level nvWf binding, whereas shear stress can …
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Dominant negative mutants of the human estrogen receptor
… co-transfection assays using ER-deficient Chinese hamster ovary cells, each of the mutants effectively suppresses the ability of wild type ER to activate transcription of an estrogen-regulated reporter plasmid. Of the three mutants, S554fs is the most potent ER inhibitor. A fourth ER …
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Three-dimensional polymeric capillary network: fabrication and applications
… regulation of metabolism in the growth of yeast cells and Chinese hamster ovary cells by controlling the density of micro-capillaries. In an effort to increase the response speed of a polymeric hydrogel device during solvent actuation, we also reported on the design, analysis, fabrication and …
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Developing Magnetic Resonance Based Analytics to Support Continuous Bioprocess
… the latter being transferrable to bench-scale. Chinese hamster ovary cells were used to provide an intracellular environment, which is found to have an associated self-diffusion coefficients 70 times slower than that of extracellular proteins. This work demonstrates how varying the NMR …
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Role of polymorphonuclear leukocytes in the tumoricidal activity of Propionibacterium acnes
… mechanism responsible for the killing of tumor cells after injection of mice with a mixture of tumor cells and Propionibacterium acnes were investigated. Tumor cells were injected intramuscularly into Balb/c mice either alone or together with P. acnes vaccine. The tumor cells were then removed …
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Identification of Novel Acid-Sensing Ion Channel 3 Modulators using in silico Modelling and Screening
… function of rASIC3 transiently transfected in Chinese hamster ovary cells using whole-cell patch-clamp electrophysiology. This *in silico* approach was also conducted for pro-inflammatory mediators known to activate/enhance ASIC3 activity, which identified potential physiological modulators. …
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