Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 97 for “"Chinese Hamster Ovary"”.
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Micro-bioreactor design for Chinese hamster ovary cells
… to design a micro-bioreactor for the culture of Chinese Hamster Ovary (CHO) cells. There is an increasing demand for upstream development in high-throughput micro-bioreactors specifically for the recombinant CHO cell line, an important cell line for producing recombinant protein therapeutics. In …
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Genetic control of death in Chinese Hamster Ovary cultures
… in mammalian cell culture systems, including Chinese Hamster Ovary (CHO) cultures, is the inability to maintain viability of these cultures over extended periods of time. This inability translates into lower final protein titers and higher protein production and recovery costs. This thesis was …
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Glycosylation site occupancy heterogeneity in Chinese hamster ovary cell culture
Thesis (Ph. D.)--Massachusetts Institute of Technology, Dept. of Chemical Engineering, 1998.
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Characterization and improvement of interferon-[gamma] glycosylation in Chinese hamster ovary cell culture
… interferon-[gamma]y (IFNY-[gamma]) derived from Chinese hamster ovary (CHO) cell culture were characterized by a sensitive (- 1 [mu]) and rapid (- 3 h) analytical methodology employing a variety of tandem chromatographic techniques, capillary electrophoresis and mass spectrometry. Although …
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Genomic Analysis And Engineering Of Chinese Hamster Ovary Cells For Improved Therapeutic Protein Production
… are produced in living cells, primarily Chinese Hamster Ovary (CHO) cells. Due to the importance of these therapeutic proteins to disease treatment, it is essential to improve the efficiency of their production, both to promote the development of new therapies, and to bring down the cost …
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The Signaling Pathway of Oxysterol Induced Apoptosis in Chinese Hamster Ovary (CHO)-K1 Cells.
… of apoptosis induced by oxLDL, we have used Chinese hamster ovary (CHO)-K1 cells treated with a potent oxysterol, 25-hydroxycholesterol (25-OHC). In the present study, we find that oxLDL can induce apoptosis in any cell types if cells present the specific receptors on their surface to take up …
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Spatial proteomic investigation of monoclonal antibody expression in the Chinese hamster ovary (CHO) cell
Over the last three decades, the Chinese hamster ovary (CHO) cell has progressively established itself as a stable, high yielding mammalian expression system for the production of recombinant proteins – in particular monoclonal antibody (mAb) biotherapeutics. Despite the availability of other …
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Ultrasonic backscatter coefficient quantitative estimates from Chinese hamster ovary and BALB/3T3 cell pellet biophantoms
… backscatter coefficient (BSC) estimate using Chinese hamster ovary (CHO), and new and old 3T3 cells. Also introduced, for its geometrical similarities to eukaryotic cells, is a concentric sphere scattering model. BSC estimates from cell pellet biophantoms of known number density were performed …
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Studies on the internalization and intracellular transport of horseradish peroxidase in Chinese hamster ovary cells
… horseradish peroxidase (HRP) is internalized by Chinese hamster ovary cells, a cell line of fibroblastic origin (Adams et al., 1982). We have confirmed this result by showing no inhibition of uptake in the presence of divalent cation chelators (EGTA Mg or EDTA), excess (19 mg/ml) yeast mannan (an …
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Identification and characterisation of a novel gene, DWNN, isolated from promoter-trapped Chinese hamster ovary cells
The process of cytotoxic T lymphocyte (CTL) killing involves the recognition and destruction of foreign antigens by cytotoxic T cells and is of crucial importance to the defence of the organism against viral infections. Defects in this process can lead to various autoimmune diseases and cancer. The …
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The Engineering of Chinese Hamster Ovary Cells to Achieve More Efficient Gene Amplification for Improving Biopharmaceutical Development
… issue of the long development times of obtaining Chinese hamster ovary (CHO) cells capable of producing high quantities of therapeutic proteins. It addresses the specific time bottlenecks associated with developing high producing CHO cell lines and reviews various methods that are employed to …
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Human Estrogen Receptor(alpha) Activity on Estrogen Responsive, Integrated Chloramphenicol Acetyltransferase (Cat) Reporter Constructs in Chinese Hamster Ovary (Cho) Cells
The estrogen receptors endogenous to CHO cells did not induce transcription of chromosomal copies of the more complex 2ERETKCAT promoter. In transient transfections hER$\alpha$ regulated transcription of the three 2ERETKCAT cell lines. We found that an ER mutant containing only AF1 elicited a dose …
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Investigating "difficult-to-express" monoclonal antibody frameworks in transient and site-specific integration-based Chinese hamster ovary cell expression systems
… to mitigate these phenotypes. mAb expression in Chinese hamster ovary (CHO) cells, the production host cell of choice, is a crucial but poorly understood developability characteristic that can be significantly impacted by amino acid sequence, leading to orders of magnitude changes in titer driven …
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The human cannabinoid CB(1) receptor stably expressed in Chinese hamster ovary cells provides a model system to predict the pharmacological effects of cannabinoids in man
… CB₁ receptor has been stably expressed in Chinese Hamster Ovary (CHO/CB₁) cells, which do not endogenously express and known cannabinoid receptors. Saturation binding studies using [³H]SR141716A and [³H]CP55,940 determined that this system expresses the human cannabinoid CB₁ receptor at …
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Early selection of Chinese Hamster Ovary (CHO) cells using simultaneous label-free autofluorescence multi-harmonic (slam) microscopy and Fluorescence Lifetime Imaging Microscopy (FLIM) for biopharmaceutical cell line development
Submission published under a 24 month embargo labeled 'U of I Access', the embargo will last until 2027-05-01
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Analysis of the roles of ligand and DNA binding in estrogen receptor action
… is unable to activate transcription. Studies of Chinese Hamster Ovary cells which were grown under serum-free conditions, suggested that commercial base media may contain traces of estrogenic compounds. Additional studies demonstrated that transcriptional synergy by ER is strongly dependent on …
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Studies on the biosynthesis of lipoic acid
… over the next five hours of aerated cell growth. Chinese hamster ovary cells were found not to incorporate the two known bacterial precursors, [²H₃]-acetate and [U-²H₁₅]-octanoate, into lipoic acid. Further studies with both Chinese hamster ovary and mouse fibroblast cells which were designed to …
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Case studies in the modeling and control of continuous pharmaceutical manufacturing processes
… or produced via recombinant hosts such as Chinese hamster ovary (CHO), Escherichia coli, or Saccharomyces cerevisiae, with some products requiring both a recombinant host and transient transfection or infection with additional genetic material. Across these modalities, industry, regulatory …
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