Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 12 of 12 for “"Chimeric receptor"”.
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Evaluating the Interaction Between the Human Melanocortin-2 Receptor and the Accessory Protein, Mrap1: Chimeric Receptor and Alanine Substitution Studies on Transmembrane Domain 4, Extracellular Loop 2, and Transmembrane Domain 5
<p>The melanocortin-2 receptor (MC2R) is the most complex due to its trafficking and ligand selectivity requirements for proper activation. The MC2R requires the melanocortin receptor accessory protein-1 (MRAP1) for proper trafficking and activation of the receptor by the melanocortin hormone, …
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Lymphocyte-specific reconstitution of IL-4Ra-deficient mice : characterization and infectious disease studies
… Human IL-4Ra may bind to mouse yc resulting in a chimeric receptor specific for human IL-4 but not mouse IL-4. This provides us with and inducible IL-4 system. The aim of this study was to investigate in vitro and in vivo characteristics of our novel hlL-4Ra Tg/mIL-4Ra+/- mouse model.
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Toward a Cell-Based Assay for Cubam-Mediated Intrinsic Factor-Vitamin B12 Binding and Uptake
… a Ba/F3 cell model for evaluating IF-Cubam receptor interactions predictive of functionality in the human digestive tract. Methods: A Cubam/EpoR chimeric receptor was expressed on the cell surface, which was expected to activate the JAK-STAT5 pathway and drive cell proliferation upon binding …
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Reengineering Estrogen Receptor Hormone Specificity Through Coordinated Changes at Key Contact Sites Between Estrogen Receptor and Estradiol
… that a hormonal ligand has with its cognate receptor. This interaction is defined by steric and electronic complementarity at certain sites at which portions of the ligand contact specific residues in its receptor. In the nuclear hormone receptors, mutational mapping studies, and more …
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Characterization of the Whale Shark (Rhincodon Typus) Melanocortin-2 Receptor
<p>Among bony vertebrates, the melanocortin-2 receptor ortholog is unique among the family of five melanocortin receptors on the basis that it is dependent on its accessory protein, MRAP1, for trafficking and activation, and is selective for activation by ACTH alone. Previous studies on the MC2R …
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Activated EphA4 receptor in <i>Xenopus laevis</i> acts rhrough src- like tyrosine kinase p59fyn to inhibit RhoA GTPase
<p>Eph receptors and their ligands, ephrins, have been implicated in many important developmental processes and in tumorigenesis. The activation of EphA4 receptors in the frog, <em>Xenopus laevis</em>, causes loss of cell adhesion, tight junctions, and stress fibers. Previous studies revealed that …
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Recombinant CXCR4/CCR5 hybrid receptors as tools for studies of HIV-1 receptor usage
The chemokine receptors CCR5 and CXCR4 are required, together with CD4, for the entry of HIV-1 into target cells. CCR5 using HIV-1 dominates during transmission and the asymptomatic phase of infection. During progression, virus phenotypes with the ability to use CXCR4 emerge in about 50% of …
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Targeting Receptor-Histidine Kinase Signaling in Staphylococcus Aureus
<p>Two-component signaling systems involving receptor-histidine kinases (RHKs) are present in all branches of life outside of the animal kingdom. Remarkably, very little is known concerning the extracellular ligands that presumably bind to RHKs to initiate signaling. The two-component agr signaling …
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Directed Migration Achieved in Mammalian Cells via Ca2+ Signal Rewiring in Synthetic Protein Chimeras
… apoptosis, motility and exocytosis. In some receptors (e.g. VEGFR2), Ca2+ signals are generated upon ligand binding (e.g. VEGF-A). Here, firstly we engineered fusion proteins that generate a Ca2+ signal upon ligand binding by creating fusions of domains that oligomerize to the transmembrane …
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Structural Basis of GPCR-Transducer Signaling
<p>G protein-coupled receptors (GPCRs) are a class of ubiquitous cell-surface receptors involved in the regulation of virtually every physiological processes. GPCRs typically signal through either G proteins or beta-arrestins (βarrs), each of which activate distinct cellular pathways that leads to …
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Non-canonical sensing mechanisms in bacteria
… to opposite pH gradients. Through in vivo chimeric receptor and site-directed mutagenesis studies, we found that the lower regions of the extracellular ligand binding domains of the chemoreceptors are involved in pH sensing. In particular, we identified number of key amino acid residues …
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Reversal of Neurodegeneration By Engineered Monocytes In Alzheimer’S Disease
… inflammation. In tumor immunity, scavenger receptors such as CD163, CD204 and FcRIIb act to limit the proinflammatory responses of the myeloid stroma. We sought to repurpose this system to facilitate o-Tau internalization in AD while limiting inflammation. Unlike microglia in late stage AD, …