Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 69 for “"Chemically defined"”.
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Osteochondral allograft preservation in a serum-free chemically-defined media
… Our work showed that storage in a serum-free chemically-defined media at 37[degrees]C can maintain OCAs \ better than the current tissue banking protocol for storage.
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Development of Chemically-Defined Platform Materials for Localized Delivery of RNA Therapeutics
Most nucleic acid therapeutics have been engineered for systemic delivery with accumulation predominantly occurring in the liver. Currently, mRNA and siRNA represent the most clinically advanced nucleic acid modalities which can be used to address underlying under- or over-expression of crucial …
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Development and use of a chemically defined medium for estimating the oxygen tolerance of campylobacter species
… such variation in complex media, we developed a chemically defined medium for Campylobacter spp. This medium allows reproducible colony counts to be obtained. The medium was used to assess the effect of ROI scavengers on the oxygen tolerance of various Campylobacter species. Allopurinol, azelaic …
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The stimulation or inhibition of growth and acid precipitable material production of Vibrio Fetus by Kreb's cycle intermediates, fatty acids, amines, and miscellaneous compounds
Using the chemically defined medium designed by Smibert (1963), the stimulatory or inhibitory effect of test compounds on the growth and the production of acid precipitable material by seven strains of vibrio fetus was determined. The test compounds were Krebs cycle intermediates, lactate, …
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Studies on the Regulation of Hepatic Hmg Coenzyme a Reductase and the Production of Anti-Hmg-Coenzyme a Reductase Monoclonal Antibodies
… cells have been adapted to growth in serum-free chemically defined Higuchi's medium for the purpose of studying the regulation of HMG-CoA reductase.
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Long-term expansion, genomic stability, in vivo safety and immunogenicity of adult human pancreas organoids
… do not expand efficiently long-term in a chemically defined, serum-free medium. The aim of this project was to generate a 3D culture system for long-term expansion of human pancreas ductal cells as hPOs to serve as the basis for studies of human pancreas ductal epithelium, exocrine …
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Nutritional, environmental and morphological studies on Trypanosoma ranarum
… in complex nutrient solutions, but growth in chemically defined media has never been attempted. The purpose of the study was to cultivate R. ranarum in “purified” bacterial media ingredients and to determine the basic nutrient requirements of the organism. Preliminary studies were also …
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Robot-trifle: A robotic system for automated combinatorial growth phenotyping
… the automated construction of minimal chemically-defined media for microorganisms known to grow in culture. The first part of the pipeline analyzes known growth data to produce media elimination experiments that are planned and executed by the second and third sections. This software …
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Sulfur amino acid nutrition of the young pig
… hydroxy analog with the same molar efficiency. A chemically defined amino acid diet (14.77% protein (N X 6.25), 3450 kcal ME/kg, 0.15% choline) in which all nutrients were 100% bioavailable was used to assess the maximal portion of the SAA requirement that could be furnished by Cys and the …
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Design of a cultivation medium for protein production in Pichia pastoris based on genome-wide biological understanding
… areas have limited investment to date. Few chemically-defined cultivation media have been reported for P. pastoris fermentation and all are minimal salt solutions.
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Susceptibility Testing of Pathogenic Fungi With a Radiometric System
… agar at 25°C and converted to yeast phase in a chemically defined, liquid medium at 37°C. Yeast inoculum was adjusted to 5x10⁴ cells. Results indicate that the radiometric method provides a reproducible, rapid, and quantitative measurement of the inhibitory effects of antifungal agents on yeast …
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DIRECT REPROGRAMMING OF HUMAN FIBROBLASTS INTO CARDIOMYOCYTES
… an optimal culture condition by defining a chemically defined media (CDM). In addition, we screened 80 transcriptional regulators and found two new factors, SALL4 and YY1 significantly enhanced reprogramming efficiency in human dermal fibroblasts (HDFs) when working together with GATA4, …
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ΙΧΝΟΣΤΟΙΧΕΙΑ ΚΑΙ ΑΦΟΜΟΙΟΥΜΕΝΑ ΑΠΟ ΤΗ ΖΥΜΗ ΣΤΟΙΧΕΙΑ ΕΛΛΗΝΙΚΩΝ ΖΑΧΑΡΟΥΧΩΝ ΠΡΩΤΩΝ ΥΛΩΝ ΚΑΙ ΜΕΛΕΤΗ ΤΗΣ ΕΠΙΔΡΑΣΗΣ ΤΟΥΣ ΣΤΗΝ ΑΛΚΟΟΛΙΚΗ ΖΥΜΩΣΗ ...
… PRODUCTION. MODEL ALCOHOLIC FERMENTATION WITH CHEMICALLY DEFINED MEDIA WERE ALSO STUDIED USING VARYING CONCENTRATION OF MACRO AND TRACE ELEMENTS. IT WAS FOUND THAT RAW MATERIALS WITH A LOW CUCONTENT (<IO PPM) SHOW BETTER KINETIC PARAMETERS THAN THOSE WITH HIGHER CU CONTENT. IN MODEL ALCOHOLIC …
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Isotopic constraints on the sources and associations of organic compounds in marine sediments
… in 14C content are observed between different chemically defined sedimentary organic fractions. These variations are dependent on organic matter inputs and/or the affects of diagenesis. Our observations also provide evidence for the protection of labile marine carbon by chemical binding.
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A revision of the genus Saprolegnia
… All isolates studied were propagated on a chemically defined medium, as well as, on halves of sterilized hemp seed (Cannabis sativa). Reliable taxonomic criteria are used in the keys provided for the identification of species. As a result of this investigation, fifteen species are …
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Development of an in vitro human lung organoid system to study alveolar type 2 stem cell maintenance and dysfunction during disease
… that allowed for long-term hAT2 maintenance in chemically-defined conditions. Cultured hAT2-derived organoids underwent clonal expansion, were functionally mature and exhibited maintenance of differentiation capacity. High Wnt signalling and FGF7 presence were found to be essential for primary …
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Iron-dependent regulation of gene expression in Corynebacterium pseudotuberculosis
… of C. pseudotuberculosis. To this end, a novel chemically-defined medium was designed, which supported the growth of C. pseudotuberculosis, but in which the concentration of specific growth factors could be manipulated. Subsequently, iron was shown to be essential for C. pseudotuberculosis …
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The crystal structure of cholesterol helical ribbons
… ribbons taken from two particular solutions: Chemically Defined Lipid Solution and model bile. Using a synchrotron x-ray source I observed Bragg reflections from ribbons with a pitch angle of 11⁰. From the diffraction patterns, I was able to deduce the parameters of the unit cell. The crystal …
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Effects of light and temperature on the formation of sexual structures in the family Saprolegniaceae
… of Saprolegnia parasitica Coker were grown in a chemically defined medium under controlled conditions of temperature and illumination. Light inhibited growth and oogonium formation rates in S. ferax and A. americana. Light inhibited zoospore germination rates in A. americana and the growth rate …
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A Comparison of Growth of Primary Mouse Marrow Explants
… been successfully maintained and grown using chemically defined media and media enriched with non-defined constituents such as horse serum and chick embryo extract. However the media which are presently available are inadequate for the support and proliferation of any single, isolated cell of …
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