Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
Results
Showing 1 to 15 of 15 for “"Chemical mutagenesis"”.
-
Chemical Mutagenesis for the Modulation and Study of Protein Function and Dysfunction within Alzheimer’s Disease
Chemical mutagenesis is defined as the post-expression interconversion of amino-acid (AA) sidechains. This approach enabled the first ever point-mutations in proteins and has seen a resurgence of interest over the last two decades. This thesis reports on two disparate applications of chemical …
-
Clonal selection and characterization of epigenetic variation in Pichia pastoris
… timely and cost-effective manner. We used random chemical mutagenesis to modify a wild-type strain of the heterologous protein production host Pichia pastoris, which resulted in overall improvement of the secretion rate of the mutated population. More than 4000 single-cells were simultaneously …
-
Essential Fatty Acid Biosynthetic Enzymes of Escherichia Coli and Lactococcus Lactis Subsp. Lactis
… report the first characterized fabG mutants. By chemical mutagenesis followed by a tritium suicide procedure, I obtained three conditionally-lethal temperature-sensitive fabG (fabGts) mutants. The E. coli mutant has two point mutations: A154T and E233K. In Salmonella enterica Serovar Typhimurium …
-
Studies of in situ nitrosative stress following nitrate ingestion in the human upper gastrointestinal tract
… whose epithelium will be more sensitive to chemical mutagenesis.
-
Identification And Characterization Of Mediators Of Toxicity Of Aβ Oligomers By Genome Wide Screening In <i>Caenorhabditis Elegans</i>
… in these worms a genetic screen following chemical mutagenesis was applied to discover the genes involved in the Aβ-dependent paralysis (forward genetics). This analysis allowed identification of a mutated clone showing a complete lack of paralysis, despite this it not bear mutations in the …
-
Dominant negative mutants of the human estrogen receptor
… a frameshift (S554fs), were generated by random chemical mutagenesis of the ER hormone binding domain and screened first for low transcriptional activity in a yeast selection system. When co-expressed with wild type ER in transient co-transfection assays using ER-deficient Chinese hamster ovary …
-
Tissue adaptations to colitis influence neoplastic risk through clonal interaction
… Such processes were explored in depth after chemical mutagenesis in the *Muc2<sup>KO</sup>* model, where both anti-inflammatory and cancer-driver mutations were shown to benefit from the permissive chronically inflamed environment for expansion. Regenerative expansions did not seem to compete …
-
Rodent inflammasome activation by Toxoplasma gondii
… clearance by nearby immune cells. Using a chemical mutagenesis screen, we identified three Toxoplasma dense granule proteins (GRAs), GRA18, GRA27 and GRA28, essential for NLRP1 inflammasome activation and pyroptosis in rat macrophages. Our work has identified Toxoplasma gondii as a novel …
-
Transcriptional Control during Quorum Sensing by LuxR and LuxR Homologues
… work describing the results of alanine scanning mutagenesis performed on the C-terminal domain of LuxR (residues 190-250) has identified residues (K198, W201 and I206) that appear to play a role in positive control of transcription initiation. Additional mutagenesis of residues 180-189 has been …
-
Genetic interactors of the SWI/SNF chromatin remodelling complex in Caenorhabditis elegans
… to a restrictive temperature. By employing chemical mutagenesis, I identified compensating mutations that can restore viability and prevent developmental defects from manifesting in these swsn-1 mutants. Firstly, I found that a specific alanine to valine mutation in the SWI/SNF core subunit …
-
The Response of Tepary Bean (Phaseolus actifolius) Germplasm to Induced Mutation
See the attached abstract below
-
Lethal and Semilethal Mutation Frequency of the First and Second Chromosomes in Two Natural Populations of "Drosophila melanogaster"
… captured in an area believed to be free of chemical contamination. Procedure. The Basc technique for determining X-linked lethal mutation frequency and the Cy/Pm method for determining second chromosome lethal mutation frequency were employed. Mutation frequencies were calculated and data …
-
Pasteurella multocida biofilm formation, and the interrelationship of P. multocida with Histophilus somni in a polymicrobial biofilm during bovine respiratory disease
… to encase large numbers of bacterial cells. Chemical mutagenesis was performed on P. multocida strain P1059, resulting in isolation of an acapsular mutant designated as P1059-R8. A uridyltransferase encoded by gene P1059_01979 was mutated in such a way that a polar amino acid was changed to a …
-
Assessment of gentle remediation options for trace element-contaminated agricultural land under semi-controlled and field conditions
… solution; chapter 4.1) and/or legally relevant chemical soil extractions (NH4NO3-solution; chapters 2 to 4) and pH measurements. Additionally, earthworms served as ecotoxicological endpoints (chapter 4.1). Initial soils, earthworms, vegetative and generative biomass produced from each approach, …
-
Putative phosphodiesterase inhibitors as potential new chemotherapies against African Trypanosomiasis
… series are inhibitors of trypanosomal PDEs and chemically validate PDEs as drug targets for potential new chemotherapies against African trypanosomiasis. The effect of PDE inhibition on the physiology of the bloodstream form trypanosomes was also investigated. Flow cytometry analysis and the …