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Showing 1 to 18 of 18 for “"Chemical ligation"”.
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Stereoselective glycosylation and chemical ligation-applications in oligosaccharide and peptide synthesis.
Stereoselective glycosylation and chemical ligation-applications in oligosaccharide and peptide synthesis.
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Synthesis of Epidermal Growth Factor by Native Chemical Ligation and Incorporation of Non-Natural Amino Acids
… complete dodecapeptide synthesis. Chemoselective ligation techniques allow the use of unprotected peptide fragments which can eliminate solubility problems associated with fragment condensation. Native chemical ligation results in a peptide bond thus, joining the fragments. Therefore, it was …
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Synthesis of Selenium-Alkylated Selenocysteine Derivatives and Their Use in Native Chemical Ligation, Expressed Protein Ligation and the Synthesis of Dehydropeptides
Finally, an improved, scalable synthesis of Fmoc-phenylselenocysteine is reported. The amino acid was coupled to a model peptide on the solid phase. It was demonstrated that the selenide could be oxidatively eliminated to give a dehydropeptide while still bound to the solid resin. In addition, the …
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Induktion von Sekundärstrukturen durch den Einbau von Alanyl-PNA in Peptide und Proteine
… von Alanyl-PNA durch die Technik der native chemical ligation in größere Peptide möglich ist. Hierfür wurde der N-terminale Thioester 31 des humanen Interleukins hIL8(1-55) durch Expression des Fusionsproteines in E.coli und Expressed Protein Ligation dargestellt. Nach Umsetzung des …
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Caged phosphopeptides and phosphoproteins : probes to dissect the role of phosphorylation in complex signaling pathways
… 31 of the 557-residue protein using native chemical ligation.
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Convergent Site-Selective Carbohydrate-Peptide Ligations With Dehydroalanine and Aziridine -2 -Carboxylic Acid-Containing Peptides
… reactions. The efficiency of the dehydroalanine ligation approach was demonstrated by the preparation of thio-isosteres of the four tumor-associated carbohydrate antigens, T N, T, STN, and 2,6-ST, as a pair of diastereoisomers at the newly formed cysteine stereocenter. This 1,4-conjugate addition …
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Chemical Modification Methods for Protein Misfolding Studies
… for misfolding studies. We adopted the native chemical ligation (NCL) method between a C-terminal thioester fragment and an N-terminal Cys fragment. We first devised strategies for the synthesis of thioamide-containing peptide thioesters as NCL substrates, and demonstrated their applications in …
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Synthesis and characterisation of peptide-based probes for quantitative multicolour STORM imaging
… anti-GFP and ant-mCherry nanobodies via native chemical ligation. The labelled anti-GFP nanobody was to demonstrate ensemble and single molecule imaging in S. pombe, as well as characterisation on single molecule surfaces in comparison to a conventional randomly labelled antibody. The …
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Identification of a Functional Hotspot on Ubiquitin Required for Stimulation of Methyltransferase Activity on Chromatin
… we developed a strategy for the site-specific chemical ligation of ubiquitin to preassembled mononucleosomes to greatly expedite structure activity studies of ubiquitin in a nucleosome context. Accordingly, we synthesized a library of H2Bligated ubiquitin alanine mutant nucleosomes and tested …
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Examining Post-Translational Modifications In Alpha-Synuclein Through Semi-Synthesis And Single Molecule Spectroscopy
… and semi-synthetic strategies involving native chemical ligation (NCL) are optimized for the incorporation of challenging or novel modifications such as phosphorylation, arginylation, and acetylation. Where applicable, we evaluate trade-offs between the synthetic ease of using a mimic and the …
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Investigation and application of heterochiral proteins enabled by flow-based peptide synthesis
… Solid-phase peptide synthesis (SPPS) and native chemical ligation enable the total chemical synthesis of proteins. Using these techniques, it is possible to design and study polypeptides foreign to Nature. Herein, I describe the investigation and application of proteins simultaneously comprised …
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DESIGN OF HIGH-YIELDING NON-ENZYMATIC DNA AND RNA LIGATION SYSTEMS
… still exists is how substrates can undergo ligation (i.e. form a covalent bond) in the absence of enzymes. The use of activated species such as imidazole and water soluble carbodiimides have been proposed as means for ligation. However, obtaining the activating agents by a prebiotic route is …
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An Inverse Electron-Demand Diels-Alder Strategy for Mapping RNA Methylation
… to investigate precisely how the installed chemical modifications impact key detection technologies, namely reverse transcription and nanopore sequencing. In this work, an inverse electron-demand Diels-Alder (IEDDA) reaction between allyl-modified nucleosides and a dipyridyl tetrazine was …
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Adiciones 1,4-conjugadas a deshidroalaninas quirales para la síntesis de aminoácidos no naturales
… of selenohomocysteine to be used in native chemical ligation is described. In addition, an amino acid bearing an alkyne group susceptible to click reactions with azides is also obtained, which can be used in bioconjugation chemistry. In Chapter 5, the synthesis of different compounds …
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A study on the interactions of synthetic IGF-II analogues with the type 1 IGF and insulin receptors.
… IGF-II protein. This was followed by an improved chemical synthesis of the Fl9Cou IGF-II protein using a linear solid phase peptide synthesis (SPPS) approach and is detailed in Chapter 4. A robust native chemical ligation approach was developed in Chapter 5, which allowed for the facile …
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Self-assembly of synthetic and biological components in water using cucurbit[8]uril
… techniques. Chapter 1 introduces the reader to chemical complexity, and how supramolecular chemists have advanced in their capability of assembling more complex molecular architectures. The discussion focusses particularly on self-assembly carried out in the aqueous phase, and how, like in …
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Studien zur Synthese einer tRNA durch chemische Ligation von RNA-Strängen
In dieser Arbeit wurde eine tRNA durch chemische Ligation von RNA-Strängen synthetisiert. Dazu wurden alle nötigen Methoden für die Synthese, Analyse und Ligation von RNA-Strängen erarbeitet.
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Metalloprotein engineering with unnatural amino acids: application in functional heme-copper oxidase and azurin
… cavity complementation, total synthesis, native chemical ligation, expressed protein ligation and amber suppression. They all have their own advantages and disadvantages. Heme copper oxidases (HCOs) catalyzes reduction of O2 to H2O and harvest energy at the end of respiration chain. The enzyme …