Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 42 for “"Chemical Crosslinking"”.
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Evaluating Chemical Crosslinking as a Tool for Enhancing DNA Damage Repair Interactome Analysis
… In this thesis, I have investigated whether chemical crosslinking incorporated into a DNA-based AP-MS workflow can improve the coverage of DSB repair interactome in S. cerevisiae. To this end, I first evaluated and optimized the RIME protocol for isolating affinity-tagged putative EBC members …
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Exploring Chemical Crosslinking of the Extracellular Matrix as a Potential Therapy for Recurrent Glioblastoma
… brain ECM to inhibit cancer cell migration chemically is possible. The study then examined brain ECM crosslinking using oxidised hyaluronic acid (oxi HA) to prevent glioblastoma cell migration. The results showed that a low concentration of oxidised hyaluronic acid gel with adipic acid …
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Physical and biochemical studies of histone H1 and its globular domain
… chromatin was studied using several physical and chemical techniques, including electron microscopy, dynamic light scattering, equilibrium density and velocity ultracentrifugation, and chemical crosslinking.
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Genipin-crosslinked electrospun collagen nanofibers
… were unstable in aqueous environments, and thus crosslinking was required. Glutaraldehyde, which is cytotoxic to cells, is currently the chemical crosslinking agent used by most research groups to stabilize collagen nanofibers. In this thesis, a novel approach, using genipin (a natural …
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Crosslinking of cotton cellulose by lignin-isocyanate copolymer
… was cured. There is emperical evidence for the chemical crosslinking of cellulose with lignin. The effects of the finish on the fabric physical properties like durable press rating, dimensional stability, tensile strength, abrasion resistance, warp and weft recovery angle were investigated. …
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DISTINCT GENOME WIDE FUNCTIONS OF CHROMATIN REMODELERS IN NUCLEOSOME ORGANIZATION AND TRANSCRIPTION REGULATION
… histone H4 Nterminal tail. Our protein-protein chemical crosslinking and mass spectrometry (CX-MS) analysis indicate that the SLIDE domain regulates the ATPase core through N terminal domains of the accessory subunit Itc1. Moreover, we show that the accessory subunits of ISWI modulate the ATPase …
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Spatially-resolved transcriptomic mapping in live cells using peroxidase-mediated proximity biotinylation
… in situ protein biotinylation with RNA-protein chemical crosslinking, while APEX-Seq utilizes peroxidase-catalyzed in situ biotinylation on RNA. We demonstrated that APEX-RIP can isolate RNAs from a variety of subcellular compartments, including the mitochondrial matrix, nucleus, bulk cytosol, …
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Probing the Ability of Flexible Hydrogels to Protect Fragile Objects
… impact. We study gels made by either physical crosslinking (e.g., gelatin) or chemical crosslinking (e.g., acrylamide) and also the same gels with various particulate additives. None of the bare gels are protective, and nanoparticles like iron oxide or silica do not help. However, the addition …
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Estratégias baseadas em reticulação de polímeros para evitar perdas de circulação em poços de petróleo
… aims to develop plugging fluids based on the crosslinking of poly (vinyl alcohol) (PVA) to seal the cracks of the rock formations and to prevent circulation loss. The study is conducted with respect to chemical crosslinking of the PVA, aiming to understand how the type of crosslinking agent, …
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Design, Production and Characterisation of Anisotropic Collagen Scaffolds for Cardiac Tissue Repair
… Protocols were established to characterise chemical crosslinking, degradation and fatigue resistance. Chemical crosslinking increased compressive Young’s modulus (*E*) from (1.69±0.60) kPa to (6.42±1.54) kPa and critical stress (σ<sub>crit</sub>) from (0.22±0.08) kPa to (0.71±0.11) kPa. …
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The design and synthesis of benzodithiophene-containing poly(arylene ethynylene)s
… poly(arylene ethynylene)s via the electrochemical and chemical crosslinking of two different 1 D-conjugated precursor polymers. We describe the characterization of the conjugated polymer network thin films and bulk materials and discuss the differences in the material properties depending …
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Photonic Properties of Liquid Crystalline Hydroxypropyl Cellulose in the Solid-state
… of functionalities. Firstly, by combining chemical crosslinking and heat treatment, photonic films across the entire visible spectrum were obtained and instead of the typical metallic iridescence associated with HPC mesophases, these films appeared matte and angular independent. Secondly, a …
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Top-Down Interrogation And Characterization Of Biological Macromolecules
… deals with gas phase fragmentation behavior of chemically modified oligonucleotides. Factors, such as the observation time-scale, the ion type and the energy deposition method, are explored using a quadrupole/time-of-flight mass spectrometer. Novel fragmentation pathway associated with negative …
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Conjugated Carbon Monolayer Membranes -- Synthesis and Integration Techniques
… solid support such as SiO2 and Si3N4 followed by chemical crosslinking within monolayer. Once linked, the membranes are robust enough to be released from the support and transferred to other surfaces. Likewise, three-dimensional objects, such as balloons and cylinders, with monolayer thickness can …
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Polymer Grafted Nanoparticles as Functional and Mechanically Robust Single-Component Composites
… bonds between PGNPs via traditional chemical crosslinking, thermal self-crosslinking, and embedding inside of a polymer network are explored as routes to functional nanocomposites.
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Anchoring Energy of Dengue E Protein into Host Membranes and Quaternary Assembly Depend upon Lipid Composition
… results from sucrose gradient sedimentation and chemical crosslinking studies comparing trimer formation in the presence of liposomes with and without anionic lipids. Though further refinement of the trimer detection assays is necessary, sedimentation results suggest that trimerization occurred …
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Conjugated carbon monolayer membranes - synthesis and integration techniques
… as silicon oxide and silicon nitride followed by chemical crosslinking within monolayer. Once linked, the membranes are robust enough to be released from the support and transferred to other surfaces. Likewise, three-dimensional objects, such as balloons and cylinders, with monolayer thickness can …
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Insights into the substrate specificities, interactions and regulatory mechanisms of bacterial glycoconjugate biosynthesis enzymes
… annotated sequence space; however, limited biochemical characterization of this enzyme superfamily has been performed. To close the gap between sequence annotation and biochemical characterization of these critical enzymes, this thesis presents methods for characterizing the substrate …
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Extracellular Matrix-Derived Microcarriers as 3-D Cell Culture Platforms
… a range of concentrations without the need for chemical crosslinking. The microcarriers were well tolerated in vivo and supported the growth of tissue-specific stromal cell populations within spinner flasks. Recognizing the multilineage differentiation capacity of human adipose-derived stromal …
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Biobased And Biodegradable Polymer Nanocomposites
… had excellent tensile and thermal properties. Crosslinking of PVA using glutaraldehyde (GA) not only increased the mechanical and thermal properties but the water-resistance. Chapter 4 describes the development and characterization of microfibrillated cellulose (MFC) based biodegradable polymer …
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