Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 24 for “"Chaperone Proteins"”.
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Investigations into the roles of bacterial TorD family chaperone proteins
… This translocase functions to transport proteins across the cytoplasmic membrane in a fully folded state. Substrates of the Tat system are targeted to the system by N-terminal signal peptides, which bear the consensus motif (S/T)RRxFLK. These proteins often require redox centres for …
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Cellular mechanisms that modulate kainate receptor trafficking and assembly
… trafficking determinants are presumed to bind to chaperone proteins, but these proteins have not been identified for any KAR subunit. Here we identified two chaperone proteins that associated with the carboxy terminus but differently regulated the localization of KA2 receptors. We found that …
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Local and global investigations into DEAD-box protein function
… RNAs do indeed misfold in the cell; however, chaperone proteins promote escape from these non-native states and foster folding to functional conformations. DEAD-box proteins are ATP-dependent RNA chaperone proteins that function by disrupting structure, which can facilitate structural …
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Regulation of autophagy, endoplasmic reticulum and unfolded protein response by glucocorticoids in physiology and disease
… of dexamethasone resulted in increase of ER chaperone proteins GRP78 and GRP94 in CEM-C7-14 cells. This is potentially related to increased cell death and may play a role in ER stress mediated cell apoptosis. Protein degradation inhibition by BTZ sensitised the ALL cells to the treatments and …
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Design of an in vitro assay to optimize assembly of nanoparticle-tagged nuclear import complexes
… formation between nanoparticle-immobilized proteins and soluble binding partners. Two chaperone proteins, importin-a and importin-3 mediate classical nuclear transport, an essential and highly conserved example of protein complex formation in eukaryotic cells. Together, these two proteins …
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Overexpression of Human Aryl Hydrocarbon Receptor in <i> E.coli</i> Using Two Different Solubility Enhancing Tags
… with AhR nuclear translocator (Arnt), the chaperone proteins in the complex get dissociated followed by the activation of transcription of various genes such as CYP1A1 and CYP1A2 by AhR-ARNT heterodimer. Various cancers have altered levels of AhR in the absence of ligand. Our current …
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Gene Expression Profiling of the nip Mutant in Medicago truncatula
… Functional analysis revealed a large number of chaperone proteins were highly expressed in the nip mutant, indicating high stress in the mutant prior to infection by rhizobia. Additionally, a database containing the information regarding the nip expression profile at both 0 days post inoculation …
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Biochemical Analysis of Evolutionary Divergent Vertebrate Larp6 Proteins
The La-related proteins (LARPs) contain a conserved bipartite RNA binding domain, called the La module, which consists of the La motif and an RNA recognition motif (RRM). Across the LARP subfamilies, the La module interacts with a variety of RNAs, allowing this protein superfamily to perform roles …
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Dual Regulation of Telomerase Activity By HSF1 And Its Role in Prostate Cancer Progression
… been shown that the key components of the hsp90 chaperone complex, including hsp90, p23, hsp70, hsp40, and HOP (p60), associate with telomerase; however, their specific roles in telomerase function and tumor progression have not yet been defined. HSF1, the primary mammalian heat shock protein …
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Scalable methods for the discovery of autophagy and disease genes
… with the ATG12-ATG5 assay, we identify two chaperone proteins as novel autophagy regulators. These chaperones act together to regulate autophagy at multiple stages, by mechanisms likely to involve stabilising the VPS34 complex and enabling DNM2-mediated autophagosome scission. Computational …
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Dissecting Mammalian Replication-Independent Chromatin Assembly — Biochemical and Structural Studies on the H3.3-Specific Histone Chaperones Hira and Daxx
<p>Histones are architectural proteins that wrap approximately two turns of DNA around their octameric core structure, constituting the fundamental packaging unit of eukaryotic chromatin — the nucleosome. Beyond their structural role, they regulate virtually all processes that act on or depend on …
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Improved production of poly(hydroxybutyrate) and related enzymes
… in insoluble and inactive deposits. Therefore, chaperone proteins were co-expressed with PhaCRe to facilitate its correct folding. This resulted in an approximately three-fold increase in the yield of PhaCRe. Another strategy for controlling the physical properties of PHAs is to blend them with …
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Role of <i>bax</i>, <i>ibpA</i>, <i>ibpB</i> and <i>cspH</i> Genes in Protecting CFT073 (Uropathogenic <i>Escherichia coli</I>) Against Salt and Urea Stress
… hypothetical protein), IbpAB (small heat shock chaperone proteins) and CspH (speculated to be DNA/RNA chaperone protein) were chosen for the current study in which their role in protecting CFT073 against salt or urea stress was investigated. First <i>bax</i>, <i>ibpAB</i> and <i>cspH</i> gene …
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Determinants of antibody specificity
… to a multicomponent PSR reagent. We found that chaperone proteins may work as well-defined, easily producible reagents with similar broad predictive power to predict downstream antibody behavior. Next, we applied these assays to assess core determinants of nonspecificity. We first analyzed a …
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THE INTERPLAY BETWEEN THE PROTEIN QUALITY CONTROL SYSTEM AND EXTRACELLULAR VESICLES IN THE DISPOSAL OF DISEASE-ASSOCIATED PROTEINS AND MIRNAS IN ALS AND FTD MODELS
… the protein quality control (PQC) system (i.e. chaperone and co-chaperone proteins, the ubiquitin proteasome system (UPS) and the autophagy). However, TDP-43 has been also found into extracellular vesicles (EVs), both large (LEVs) and small (SEVs), suggesting that EVs could cooperate with the …
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Branching into RNAi: Synthesis, Characterization and Biology of Branch and Hyperbranch siRNAs
… silenced, thereby inhibiting the translation of proteins that can lead to the production of pathological disorders such as cancer. In spite of their utility, several challenges still remain towards the development of a fruitful cancer-targeting gene therapy approach.</p> <p>Here, a new class of …
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Poly(Arginine) Derived Cancer-Targeting Peptides for the Development of a Cancer-Targeted Gene Therapy Approach in HepG2 Liver Cancer Cells
… GRP78 is a member of the heat shock family of chaperone proteins, assisting in protein folding events under physiological stress induced conditions that are mitigated by the unfolded protein response (UPR) mechanism. In cancer, GRP78 is overexpressed and cell surface localized where it …
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Investigating the effects of calreticulin and beta-galactosidase on microglial functions and neuronal loss
… by calreticulin and β-galactosidase, two proteins associated with ageing, chronic inflammation, and neurodegeneration. The aggregation of amyloid-β to form oligomers and insoluble amyloid plaques in the brain is a hallmark of AD. Amyloid-β can be directly neurotoxic and induce …
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Analogues of Nitrofuran Antibiotics are Potent GroEL/ES Pro-drug Inhibitors with Efficacy against Enterococcus Faecium, Staphylococcus Aureus, and Escherichia Coli
In 2019, the ESKAPE pathogens were highlighted by the World Health Organization as some of the most prominent threats to human health, capable of developing significant antibiotic resistance. These pathogens contribute to over 2 million annual infections and ~23,000 annual deaths in the U.S. In …
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