Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 34 for “"Chaperone Protein"”.
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Assembly and biochemical properties of a human chaperone/co-chaperone protein complex
The 70kDa members of the heat shock protein family (eg. Hsp70) function as molecular chaperones by binding to exposed hydrophobic patches on nascent polypeptides forming non-covalent interactions, thereby preventing their aggregation and facilitating their proper folding. The folding reaction …
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Elucidation Of The Functional Role Of Pcbp-The Cytosolic Iron Chaperone Protein Family, In Cellular Iron Homeostasis
… life and often utilized as a cofactor in many proteins. In humans, iron accumulation causes cirrhosis, arthritis, cardiomyopathy and diabetes mellitus, and it is associated with increased risk of cancer and heart disease. In contrast, decreased brain iron content results in permanent …
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The Chlamydia Trachomatis Protein Interaction Network: Insights into the Unique Composition of the Type Three Secretion System
… system to identify and characterize chlamydial proteins involved in virulence mechanisms. In this study we describe the identification of twenty-eight proteins potentially involved in modulating host cellular functions and the secretion of virulence factors into the host. Since the delivery of …
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An E. coli small RNA inhibits translation initiation from a distance
… competes with the initiating ribosomes while the chaperone protein Hfq plays a supporting role. Contrary to this widely accepted model, there are a few examples in the literature where the sRNA base pairs far from the SD region, yet translation of the target mRNA is still inhibited. …
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Inhibition of Hsp90 in Streptomyces coelicolor
Inhibition of the chaperone protein Hsp90 in plants and insects has been found to result in drastic changes in phenotype. We investigated the effect of Hsp90 inhibition on the bacteria Streptomyces coelicolor. These changes were studied by growing S. coelicolor in the presence of Hsp90 inhibitors, …
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Exploring the Mitigation of TDP-43 Toxicity by Sis1 in Yeast
… cure. Research has revealed that Tar DNA-binding Protein 43 cytoplasmic aggregates are involved in many cases of Amyotrophic Lateral Sclerosis. One possible mechanism for TDP-43 induced toxicity is that these aggregates are titrating away a molecular chaperone protein, Sis1, from the nucleus of …
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Characterization of a novel pro-apoptotic role for nuclear associated BCL-2
… of binding between Bcl-2 and its mitochondrial chaperone protein FKBP38. My work has characterized the function of nuclear-associated Bcl-2 as a pro-apoptotic protein and Bcl-2’s BH4 domain as a critical domain for binding the chaperone protein FKBP38. Based on these findings we attempted a …
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RNA interference targeting Glucose-Regulated-Protein 78 induces HepG2 cell Apoptosis
… The over-expression of an endoplasmic reticulum chaperone protein, 78-kDa glucose-regulated-protein (GRP78), has been observed on the surface of cancer cells, but not on normal tissues. By selectively targeting GRP78 with short-interfering RNA (siRNA), potent GRP78 silencing is anticipated by the …
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Regulation of Expression and Function of Neuronal Nicotinic Receptors by Accessory Subunits
… (α4)2(β2)3 by overcoming the effects of a native chaperone protein that otherwise facilitates (α4)3(β2)2 trafficking. Furthermore, we hypothesized that in the (α3)3(β4)2 stoichiometry, the α3 accessory subunit forms a third ligand binding site as observed in the paralogous α4β2 nAChR. To study …
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Novel Design Strategies For Engineering Biliverdin-Binding Fluorescent Proteins
Fluorescent proteins (FPs) have revolutionized our ability to image biological systems. However, poor penetration and high autofluorescence of visible light in mammalian tissue has motivated the design of FPs that can take advantage of the minimal autofluorescence, scattering, and absorption in the …
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Dual Regulation of Telomerase Activity By HSF1 And Its Role in Prostate Cancer Progression
… been shown that the key components of the hsp90 chaperone complex, including hsp90, p23, hsp70, hsp40, and HOP (p60), associate with telomerase; however, their specific roles in telomerase function and tumor progression have not yet been defined. HSF1, the primary mammalian heat shock protein …
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Targeting the calcium ATPase to the endoplasmic reticulum
… PMCA sequence results in<br/>mistargeting of the protein to the plasma membrane. The opposite construct (consisting of<br/>PMCA with the C-terminus replaced by that of SERCA) is located in the ER, suggesting that<br/>the ER retrieval signal lies towards the C-terminus of the protein. Many of the …
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Regulation of autophagy, endoplasmic reticulum and unfolded protein response by glucocorticoids in physiology and disease
… reticulum stress (ER stress) and unfolded protein response (UPR) respectively, in acute lymphoblastic leukemia cell lines including CEM-C1-15, CEM-C7-14 and Molt4. The results showed that autophagy inhibition by CLQ increased cell death and combination treatment with Dex exerted cytotoxic …
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Development of West Nile virus candidate vaccines in Nicotiana benthamiana
… be a very cost-effective means of making complex proteins. Plants can produce and modify proteins in a similar manner to mammalian cells and production does not require sterile conditions or specialised facilities. We propose that plants could be a viable means of making feasible, low-cost …
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Regulation of Mammary Gland Development and Tumorigenesis By 14-3-3 Zeta
… and <em>in vitro</em> approaches. 14-3-3z is a chaperone protein that interacts with a multitude of oncogenes and tumor suppressor genes, thereby functioning as a critical node in multiple oncogenic signaling networks. Mammary gland-specific 14-3-3z transgenic mouse models showed that 14-3-3z …
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Hepatitis C Virus NS5A Targets the Nucleosome Assembly Protein NAP1L1 to Control the Interferon Response
… a chronic infection. The non-structural viral protein NS5A has the capacity to interact with a large number of cellular factors involved in promoting viral replication/assembly and in the cell antiviral response to HCV. Interaction of NS5A with the nucleosome assembly protein NAP1L1 has been …
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Pilus biogenesis in uropathogenic Escherichia coli : An electrophysiology study on the PapC usher
… the polymerization of different subunits. The chaperone/usher pathway is a bi-component secretion system that is dedicated to the biogenesis and export of pili to the cell surface. It is comprised up of a periplasmic chaperone protein and an outer membrane usher protein that functions as the …
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Inhibition of Heat Shock Protein 90 Reduces Inflammatory Signal Transduction in Murine J774 Macrophage Cells and Lessens Disease in Autoimmune MRL/lpr Mice: What in vitro, in vivo, and in silico Models Reveal
Heat shock protein 90 (HSP90) is a molecular chaperone protein that protects proteins from degradation, repairs damaged proteins, and assists proteins in carrying out their functions. HSP90 has hundreds of clients, many of which are inflammatory signaling kinases. The mechanism by which HSP90 …
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Regulation of sterol regulatory element binding protein-1 in bovine mammary epithelial cells
… factor sterol regulatory element binding protein-1 (SREBP1) plays a central role in milk fat synthesis. SREBP1 stimulates the transcription of genes encoding lipogenic enzymes. The overall objective of these studies was to investigate the mechanisms of SREBP1 regulation by nutrients. In …
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Identification and Characterization of the Hfq protein and small RNAs in Francisella novicida
… were used to both determine the role of the RNA chaperone protein Hfq and identify potential novel small RNAs in <italic>F. tularensis</italic> subsp. <italic>novicida</italic> strain U112. The Hfq protein is recognized as an important regulatory factor in a variety of cellular processes, …
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