Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Channel protein"”.
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Aquaporin 3 Water Channel Protein Gene Expression in Squalis Acanthias Dogfish Shark
Aquaporins are a family of membrane proteins that provide channels for the rapid movement of water molecules and some small solutes across the cellular membrane. Since the discovery of the first water channel protein, hundreds of homologous proteins have been found in all forms of life and much has …
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Structural Analysis of Transient Receptor Potential Vanilloid Type 1 (TRPV1) Channel Protein and Proline Mimics using Computational Techniques
… Transient Receptor Potential (TRP) family of ion channels encompasses more than 30 members, which are expressed in many different tissues and cell types.<sup>1</sup><strong> </strong>Transient Receptor Potential Vanilloid Type 1 (TRPV1) is part of the TRP family gated by vanilloids, heat and …
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Extracting Equilibrium From Nonequilibrium: Free Energy Calculation From Steered Molecular Dynamics Simulations
… is then applied to a process involving an actual protein, glycerol conduction through the membrane channel protein GlpF. From the potential of mean force thus obtained, important observables such as the binding constant and conductivity are estimated. And finally, the problem of finding reaction …
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Imaging biological water permeability barriers using CARS microscopy
… The vessels of mice deficient in the water channel protein aquaporin 1 (AQP1) were examined to evaluate the role of AQP1 in vascular water permeability. A layered hydrogel imaging phantom was constructed from poly(ethylene glycol) diacrylate (PEGDA) to validate ability of water-CARS …
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A translational approach to understanding cellular responses to vascular injury
… arteries after vascular injury. Connexin 43 is a channel protein that facilitates cell to cell communication and regulates cell proliferation as well as migration, yet it's role in vascular cell types is poorly understood. Here, I hypothesized that Cx43 and its functional regulation by kinases …
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Modeling and Simulating Biomolecular Machineries at Atomic Scale and Beyond
… the directional translocation; (2) a membrane channel protein Aquaporin-1 (AQP1), conducting water through its individual monomeric pores, for which we have proposed a feasible mechanism of gating and ion conduction in its tetramerical central pore; (3) a four-way DNA junction (Holliday …
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Extracting Equilibrium From Nonequilibrium: Free Energy Calculation From Steered Molecular Dynamics Simulations
… of life is composed of molecules such as DNA and proteins. Technology has brought us today to the stage where one can investigate biomolecules at the single-molecule level. Atomistic simulations and experimental techniques such as atomic force microscopy and optical tweezer have already proved to …
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STUDIES ON INSECTICIDE RESISTANCE IN TUTA ABSOLUTA (MEYRICK), WITH SPECIAL EMPHASIS ON CHARACTERISATION OF TWO TARGET SITE MECHANISMS
… (point mutations) in the para-type sodium channel protein leading to reduced sensitivity of the insect nervous system to the pyrethroids. Mutations in the Ace gene have also been reported to cause insensitivity to organophosphates. A combination of PCR-based molecular methods and …
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Functional Asymmetry of The Immune Cell Plasma Membrane
… and destroyed by energy-independent lipid channels called scramblases. Although this compositional asymmetry has been known for decades, there has been little investigation of its structural impact on the physical properties of the membrane, nor its functional impact in healthy cells. Here, …
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Development of biomimetic, hybrid protein-synthetic membranes: determining protein insertion efficiency, membrane material suitability and relevance of aquaporin Z
… Biomimetic membranes consist of membrane proteins, such as the bacterial water channel protein aquaporin Z (AqpZ), embedded in a lipid bilayer or a polymer that mimics a cell’s natural lipid bilayer. Permeability and stability pose critical barriers to implementation of biomimetic …
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The Intersection of Neurodegeneration and Mitochondrial Stress in Caenorhabditis Elegans Models of Parkinson's Disease
… of PD is the accumulation of the misfolded protein, α-synuclein (α-syn). Emerging evidence suggests an interplay between α-syn and mitochondria leads to a disruption of mitochondrial function, impaired energy production, and increased oxidative stress. During stress, the transcription …
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Rapid Non-genomic Actions of Cortisol in Fish
… with the calcium release-activated (CRAC) channel may be responsible for the rapid increase in [iCa2+] due to cortisol. This increase in [iCa2+] with cortisol played a key role in the translocation of intracellular GR to the plasma membrane. This was evident because inhibition of the CRAC …
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Modulation of calcium channel expression in cardiac myocytes of rats during growth hormone-induced growth or normal postnatal development
… current properties and constant single T-channel current suggest that the change in T-current density is likely due to a change in membrane density of T-type Ca$\sp{2+}$ channels rather than a change in the channel protein itself. Though atrial T-current density increases when plasma …
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Neurotoxic effects of malathion and lead acetate on the blood-brain barrier: Disruptive effects caused by different mechanisms examined with an in vitro blood-brain barrier system
… suggests that disruptions involve changes in protein levels and gene expression as well as activation of transient receptor potential canonical channels (TRPC) that in turn increase intracellular calcium levels affecting tight junction structure. The hypothesis was tested by assessing …
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Investigation of stability, dynamics and scope of application of mycobacterial porin MspA: a highly versatile biomolecular resource
… smegmatis (MspA) is an octameric trans-membrane channel protein and is one of the most stable porins known to date. MspA has been successfully isolated and purified to obtain liquid extracts and crystals using a modified extraction procedure. A full analytical assessment has been carried out to …
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Functional and Structural Studies of the Human Voltage-Gated Proton Channel
<p>The activity of voltage-gated cation channels underlies the action potentials that allow for neuronal signaling and muscle contraction. The canonical family of voltage-gated K<sup>+</sup>, Na<sup>+</sup> and Ca<sup>2+</sup> channels has been the subject of extensive electrophysiological, …
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Studies on voltage-gated sodium channel β3 subunit structure and cancer-related functions using single-chain variable fragment antibodies and bioinformatics
Voltage-gated sodium channels (VGSC), embedded in the plasma membrane of cells, play a pivotal role in generating sodium currents and action potentials. The mammalian VGSCs consist of a large pseudo-tetrameric pore-forming α subunit, the channel protein, which associates with one or more …
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Action of halothane on large-conductance, calcium-activated potassium channels in rat cerebrovascular smooth muscle cells
… of large conductance, Ca²⁺-activated potassium channels (BK channels) in cerebral artery smooth muscle cells. The present experiments utilized extra cellular patch clamp techniques to investigate whether clinically relevant concentrations of halothane directly alter the biophysical properties of …
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Examination of Placenta and Child Saliva Samples Associated with Childhood Allergy Development
… placental genes are indirect measures of altered protein concentrations in the saliva of children with allergies. The aim of this PhD thesis was to identify proteins associated with childhood allergy using two sources of biological tissue: placental tissue and child saliva. The long term …
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