Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"Channel Gating"”.
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Molecular Determinants of TRP Channel Gating
<p>Transient Receptor Potential (TRP) ion channels are a family of proteins that contribute to a myriad of physiological functions. They have been extensively studied since their discovery 35 years ago; however, there are still many questions regarding the mechanisms by which they function. This …
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Characterization of cardiac IKs channel gating using voltage clamp fluorometry
Voltage-gated ion channels make up a superfamily of membrane proteins involved in selectively or non-selectively conducting charged ions, which can carry current in and out of cells, in response to changes in membrane voltage. Currents carried by ion channels influence the voltage across the cell …
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The Extracellular Domains of the AMPA, Kainate, and Orphan Delta Receptor Regulate Channel Gating in Distinct Ways
… glutamate receptors are ligand-gated cationic channels essential for neuroplasticity and neurological functions such as learning, memory, behavior, cognition, and motor coordination. Mutations in these channels are linked to various disorders including autism, ataxia, and epilepsy, making them …
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Solid-state nuclear magnetic resonance investigations of the influenza M2 protein : structure and dynamics characterization of channel gating and conduction
… In this thesis, the mechanisms of conduction and gating are investigated in the influenza AM2 and BM2 proton channels bound to native-like lipid bilayers. Using pH-dependent 15N chemical shifts of the conserved Histidine residue, we have found that the proton conduction mechanism is the same in …
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Mechanistic investigation of coordinated conformational changes in multisubunit ion channels and enzymes
Many enzymes and ion channels consist of multiple subunits and/or multiple distinct functional components. Coordinated conformational changes through allosteric interactions between subunits and/or between functional units can efficiently regulate protein activity. This dissertation describes …
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Light Stimulation of Sensory Hair Cells
… Their mechanosensitivity arises from direct gating of force-sensitive ion channels by the tension in tip links located at the tops of the stereocilia. The study of hair cells and their mechanically gated channels has heretofore required physical displacement of a hair bundle to open its …
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Exploring the Dynamics of Mechanosensitive Channels Utilizing a Post Translational Modification Approach
… in osmolarity. The bacterial mechanosensitive channel of large conductance (MscL) and the mechanosensitive channel of small conductance (MscS), from E. coli, have been the most studied mechanosensors to date. These channels coordinate a response to rapid changes in osmolarity to serve as …
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The emerging role of the a4(+)a4(-) interface as a determinant of functional signatures of a4ß2 nicotinic acetylcholine receptors
… Being heteromeric pentameric ligand-gated ion channels (pLGIC), a4p2 receptors are activated by binding of agonist to sites located at the a4( + )/P2( -) interfaces. These interfaces are present in both stoichiometries they are unlikely to contain structural differences conferring specific …
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Cholesterol Modulation of BK Currents and Cerebral Artery Diameter Via Channel-Forming Alpha-Subunit
… and calcium-gated, large conductance potassium channels (BK; MaxiK) are ubiquitously expressed and subsequently mediate numerous physiological processes. Cholesterol is consumed in high amounts with the diet and accumulates in cellular membranes when plasma levels become elevated. Cholesterol is …
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Decoding Gating Properties of the Pannexin1 Channel: ATP Release and pH Sensitivity and the Potential Role in Health and Disease
… next. In a cell model Panx1 showed pH dependent channel gating properties. Site directed mutagenesis and high throughput dye uptake tests revealed a conserved domain of the protein as a pH sensor. Further, the critical role of a single histidine residue in this domain was determined. In summary, …
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Computational Studies of the Concealed Gating Pathways and Electrophysiological Function of the Human Cardiac IKs Channel
… approach, we study molecular processes of ion-channel gating using IKs as a paradigm. The simulations reproduce experimentally recorded saturation of gating charge displacement at positive membrane voltages, two-step voltage sensor movement shown by fluorescence, ion-channel statistics, and …
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Lipid Chemistry and Mechanical State of the Membrane Modulate Ion Channel Function
… focused on voltage-dependent K<sup>+</sup> (Kv) channels. Kv channels serve many different functions in different cells, but most notably underlie action potentials in electrically excitable cells, such as neurons and muscle (Hodgkin and Huxley, 1952, 1945). Kv channel gating is governed by the …
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Structural Studies of the Cold and Menthol Sensing Ion Channel TRPM8
… sensory circuit is mediated by receptors or ion channels expressed at peripheral nerve endings, which respond to specific chemical, thermal, or mechanical stimuli and trigger ion fluxes that lead to firing of action potentials for signal transduction. Understanding the mechanisms by which these …
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Dynamics and Model of the Pore-Forming Protein Lysenin
… in both a soluble and membrane phase. Lysenin channels possess several important properties characteristic of ion channels without the inherent difficulties that plague investigations with biologically vital membrane transporters like voltage-gated ion channels.</p> <p>Work described here …
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Phosphorylation kinetics of cardiac gap junction regulation during stress
… terminus can alter the subcellular localization, channel gating, and internalization of Cx43. The channel open probability of gap junctions is regulated, in part, by the phosphorylation of S368. Phosphorylation of S365 and S373 have been reported to exert gatekeeper effects on the phosphorylation …
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Engineering Hv1 Channel Mutations and Targeted Drug for Treatment of Hv1 Mutation Associated with Cancer
The voltage-gated proton channel Hv1 is crucial for maintaining cellular pH homeostasis by regulating proton movement across membranes, playing a vital role in acid extrusion and intracellular pH regulation. Dysregulation of Hv1 is associated with various diseases, including cancer, where it helps …
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Structure and Function of the Alpha3Beta4 Nicotinic Acetylcholine Receptor
… receptors are pentameric ligand-gated ion channels that are essential for the proper function of the central and peripheral nervous systems. The α3β4 subtype is highly expressed in the autonomic ganglia, where it contributes to signal transduction from the central nervous system to the …
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Modulation of The Receptor Gating Mechanism and Allosteric Communication In Ionotropic Glutamate Receptors
… nature, large size of the protein, complex gating mechanism and inadequate knowledge regarding the conformational dynamics of the receptors during channel gating mechanism have been some of the limiting factors in elucidating the structure-function relation of iGluRs. Thus, to understand the …
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Magic angle spinning NMR applied to membrane protein 2D crystals : the structure and function of VDAC
… protein VDAC, the Voltage Dependent Anion Channel. Our results on VDAC have relied on a number of improvements in NMR methodology described in this thesis. These new methods include '3C-' 3C and 5N- 3C recoupling experiments optimized for measuring long-range homonuclear and heteronuclear …
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