Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Chalcone Synthase"”.
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Characterization of chalcone synthase genes in red raspberry cultivar (rubus idaeus)
… compounds involves type III polyketide synthases (PKSs): chalcone synthase (CHS), which is the first enzyme of the flavonoid synthetic pathways leading to synthesis of various anthocyanins. The European red raspberries are a commercially important fruit that needed both for fresh fruit …
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Endogenous short interfering RNAs that silence chalcone synthase result in pigment pattern formation on soybean seed coats
… dominant alleles correspond to duplications of chalcone synthase (CHS) genes leading to production of CHS siRNAs which in turn degrade CHS mRNAs resulting in yellow seed coats (Tuteja et al., 2009 and Vodkin et al., 2012). However, it was not known if the same phenomenon applied to the yellow …
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Exploring protein interactions and intracellular localization in regulating flavonoid metabolism
… to probe the role of subcellular localization of chalcone synthase, the first committed flavonoid biosynthetic enzyme, on metabolic flux, and to characterize a novel chalcone synthase-interacting protein. The over-expression of this interacting protein induces novel phenotypes that are likely …
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Investigating Extreme Lifestyles Through Mangrove Transcriptomics
… characterized phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), and dihydroflavonol reductase (DFR) gene families in R. mangle , which are at major regulatpry points in the phenylpropanoid pathway. I have identified one DFR, two CHS, and ten PAL genes in R. mangle . Differential …
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Dual-omics approach for 3,4-dihydroisocoumarin characterisation from Hydrangea serrata and isolation of polyketide synthases for heterologous expression in Pseudomonas putida KT2440
… biocatalysis mechanism is well known from the chalcone synthase superfamily, a group of plant specific Type III polyketide synthases. Chalcone synthases and their homologues, the stilbene synthases produce a huge amount of specialised plant metabolites including phenylpropanoids. The …
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Ecological and Genetic Status of the Purple Pitcher Plant, <i>Sarracenia purpurea</i> L., in Maryland and Virginia
… restriction fragments from the intron of the chalcone synthase gene to compare <em>S. purpurea</em> populations and determine whether the subspecies concept was supported. I performed a census of existing populations, compiled all known historical data on the species, and investigated the …
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MOLECULAR ANALYSIS OF FLORAL PIGMENTATION IN THE ORCHID, BROMHEADIA FINLAYSONIANA
… encoding phenylalanine ammonia-lyase (PAL), chalcone synthase (CHS), flavanone 3-hydroxylase (F3H) and dihydroflavonol 4-reductase (DFR) were then isolated by screening the cDNA library with respective polymerase chain reaction (PCR) generated homologous probes. Southern blot analysis …
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Differential expression of small RNAs in soybean
… seed coats (Tuteja et al., 2009) and that target chalcone synthase mRNAs for down regulation leading to absence of pigment in the seed coats. In addition, several other novel miRNAs and siRNAs were shown to be expressed differentially in seed coats versus cotyledon including highly abundant small …
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Enzyme structure, function, and evolution in flavonoid biosynthesis
… and biochemically characterized orthologs of chalcone synthase (CHS), the enzyme that catalyzes the first step of flavonoid biosynthesis, from diverse plant lineages. By doing so, we gained insight into the sequence changes that gave rise to increased reactivity of the catalytic cysteine …
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Development of antibodies for characterizing the Arabidopsis flavonoid biosynthetic pathway
… cDNAs from Arabidopsis coding regions of chalcone synthase (CHS) and chalcone isomerase (CHI) were sub-cloned in frame into a bacterial expression vector as fusions with glutathione Stransferase (GST) using standard directional cloning techniques. Analysis of crude extracts of Escherichia …
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Structural variation at soybean loci regulating small RNAs and seed color
… is composed of an inverted-repeat cluster of six chalcone synthase (CHS) genes on chromosome 8 whose unique arrangement generates CHS siRNAs that downregulate target CHS7 and CHS8 genes on non-linked chromosomes resulting in yellow seed coats. Chapter 1 presents a transition in size of the siRNAs …
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Profiling phytochemical and nutritional components of potato
… of expression of phenylalanine ammonia-lyase and chalcone synthase were higher in varieties accumulating high contents of phenolic compounds. However, levels of expression of phytoene synthase and L-galactono-1,4-lactone dehydrogenase were not different between varieties showing contrasting levels …
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Early land plant origins of the MYB-bHLH-WDR complex
… of early flavonoid pathway genes such as *Chalcone Synthase*, could have been the ancestral function. We find evidence that duplication of the *MYB* member has repeatedly been associated with neofunctionalization of the complex in rosids, for trichome and root hair patterning, and in …
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The molecular basis of seed coat pigmentation in Glycine max
… kb Hind III polymorphism related to CHS1 of the chalcone synthase (CHS) multigene family. Additionally, in many cases, mutations from the $i\sp i$ allele to the recessive i allele can be associated with the absence of a 2.3 kb Hind III CHS fragment which represents CHS4. Moreover, analysis of …
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Molecular Genetic Analysis of Cyclic GMP Signaling in Higher Plants
… interact to induce the expression of chalcone synthase. A novel genetic screen was developed based on a phytochrome-dependent phenotype induced in constant red and far-red light by the cGMP-dependent phosphodiesterase inhibitor zaprinast in Arabidopsis thaliana. Mutations at a locus …
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Morphological and Biochemical Analysis of Variation in Diploid Glycine Tomentella Hayata (2N = 38, 40)
… with three P32 labeled, genomic DNA probes (Chalcone synthase, soybean seed urease, and soybean lectin). Genomic DNA was also amplified with the Polymerase Chain Reaction (PCR) using primers tailored to one intron of the a' subunit of b -conglycinin These PCR products were digested with …
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Structural Characterization of the Flavonoid Enzyme Complex
… characterize a series of loss-of-function chalcone synthase (CHS) alleles and to define the molecular basis of the interaction between CHS and the second enzyme of flavonoid biosynthesis, chalcone isomerase (CHI). CHS proteins encoded by five previously characterized alleles were …
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The transcriptional regulation and health benefits of anthocyanin in Actinidia (kiwifruit) species
… two key genes from the core anthocyanin pathway, chalcone synthase and flavonoid 3-O-glycosyltransferase, were essential for the biosynthesis of anthocyanin. Two MYB TFs, MYB110 and MYB5, and the WRKY44 TF were involved in the regulation of anthocyanin biosynthesis. Collectively, MYB110 regulates …
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The Utilization of the Hmg2 Inducible Promoter to Genetically Engineer Parasite Resistance in Tobacco
… parasitism. The bean (Phaselous vulgaris L.) chalcone synthase 8 (CHS8) promoter has been characterized as a broomrape–responsive promoter. We introduced the CHS8:TMV replicase gene construct into tobacco plants that contains an endogenous N gene. Transgenic tobacco plants were inoculated with …
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The relationship between fruit color traits and anthocyanin genes in diploid Fragaria
… of structural genes coding for the enzymes: chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), anthocyanindin synthase (ANS), and one Del-like regulatory gene (RAN) in the anthocyanin biosynthetic pathway were amplified, cloned …
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