Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 7 of 7 for “"Chalcone Isomerase"”.
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Immunomodulation of Flavonoid Biosynthesis in Transgenic Arabidopsis thaliana
… from a phage display library using Arabidopsis chalcone isomerase (CHI) of the flavonoid biosynthetic pathway as the antigen. Each of the genes encoding the scFv's was cloned into a plant transformation vector, which was subsequently used to generate transgenic plants. One transgenic line with …
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Development of antibodies for characterizing the Arabidopsis flavonoid biosynthetic pathway
… cDNAs from Arabidopsis coding regions of chalcone synthase (CHS) and chalcone isomerase (CHI) were sub-cloned in frame into a bacterial expression vector as fusions with glutathione Stransferase (GST) using standard directional cloning techniques. Analysis of crude extracts of Escherichia …
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Structural Characterization of the Flavonoid Enzyme Complex
… characterize a series of loss-of-function chalcone synthase (CHS) alleles and to define the molecular basis of the interaction between CHS and the second enzyme of flavonoid biosynthesis, chalcone isomerase (CHI). CHS proteins encoded by five previously characterized alleles were …
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Proteomic Analysis of the Flavonoid Biosynthetic Machinery in Arabidopsis Thaliana
… complex. One of the key flavonoid enzymes, chalcone isomerase (CHI), was used in these experiments to detect interacting enzymes. Recombinant thioredoxin (TRX), or TRX-CHI, was produced in E. coli, then purified by metal affinity chromatography, and covalently coupled to an activated resin, …
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The relationship between fruit color traits and anthocyanin genes in diploid Fragaria
… of structural genes coding for the enzymes: chalcone synthase (CHS), chalcone isomerase (CHI), flavanone 3-hydroxylase (F3H), dihydroflavonol 4-reductase (DFR), anthocyanindin synthase (ANS), and one Del-like regulatory gene (RAN) in the anthocyanin biosynthetic pathway were amplified, cloned …
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Molecular Genetics and Subcellular Localization of Flavonoid Metabolism in Arabidopsis
… the first enzyme of flavonoid biosynthesis, chalcone synthase (CHS), were characterized in an effort to develop tools for investigating the organization of flavonoid metabolism in Arabidopsis. A variety of mutant CHS genotypes were identified in this allelic series, including ones that …
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Identifying and characterizing genes and transcription factors involved in coloration of apple fruit
… biosynthesis pathway by down-regulating chalcone isomerase (CHI), dihydroflavonol reductase (DFR), and leucoanthocyanidin reductase (LAR) genes. Moreover, the observed loss of flower color in transgenic tobacco lines was attributed to reduction of anthocyanin pigments. This was likely due …