Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 16 of 16 for “"ChR2"”.
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Integrating Genomics and Metabolomics to Inform Breeding for Powdery Mildew Resistance in Grapevine
… 30% phenotypic variation. Marker haplotypes, hap+chr2 and hap+chr15, were constructed to trace the inheritance of resistance loci in grandparent-parent-progeny relationships. Both hap+chr2 and hap+chr15 in the resistant F1 progeny were inherited from parent MN1264, that originated from grandparent …
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Opto-mechanical control of nerve growth
… ganglia (DRGs) expressing channelrhodopsin 2 (ChR2) as a test system, I have investigated a broad range of optical stimulation parameters and identified conditions that enhance neurite outgrowth by three-fold as compared to unstimulated controls or wild-type DRGs lacking ChR2. I have also found …
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Bis(η1,η5 -pentafulven)titankomplexe: Reagenzien für selektive Reaktionen mit N–H-aciden Substraten und Katalysatoren in der intramolekularen Alkenhydroaminierung
… diese Weise konnte das Hydrazidotitanocen (C5H4–CHR2)2Ti=NN(Ph)2(py) kristallographisch charakterisiert werden. Bei der Reaktion von 1 mit 1,1-Diphenylhydrazin und Pyridin (py) zu (η5-C5H4–CHR2)Ti(=NN(Ph)2)(–NN(Ph)2)py wurden drei unterschiedliche Ti–N-Bindungen gebildet.
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Optogenetic dissection of the dopaminergic circuitry involved in memory consolidation
… were selectively tagged with channelrhodopsin-2 (ChR2). Conditional ChR2 expression made it possible to reliably identify CAergic neurons during unit recording sessions in freely moving animals. The main conclusion of the study is that that CAergic neurons in both VTA and LC selectively increase …
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Homology Cloning, Heterologous Expression and Characterization of A New Channelrhodopsin
… named <em>Cr</em>ChR1 and <em>Cr</em>ChR2, respectively. Our goals are to expand knowledge of channelrhodopsin mechanisms and also to identify new channelrhodopsins from various algal species with improved properties for optogenetic use. For these aims we are targeting algae from …
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Reconfigurable neural probes for chronic electrical recording
… in the rat model and in the optogenetic Thyl-ChR2-YFP mouse model. The electrical and mechanical functionality of the probe was confirmed under acute in vivo conditions in the medial prefrontal cortex of optogenetic Thyl-ChR2-YFP mice. Neural activity was successfully recorded from the probe …
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Analysis of neural circuits in vitro
… ion channel channel rhodopsin-2 (ChR2) promised to revolutionize the way in which we could map connectivity in vitro. We successfully delivered the gene for ChR2 in hippocampal cultures using recombinant adeno-associated virus and characterized the spatial resolution, as well as …
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Fast scanning two-photon microscopy
… to the neuron cell body was tested using a ChR2-MBD construct.
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Expression of microbial rhodopsins in retinal neurons with subcellular targeting motifs: for the study of the structure/function of aii amacrine cells and for vision restoration
… expression of channelrhodopsin-2-GFP (ChR2-GFP) with the AIS-targeting motif of sodium channels (NavII-III) and was shown to be the site of spike initiation. The second part of the dissertation aimed to improve microbial rhodopsin-mediated gene therapy for vision restoration by using …
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Development and application of control tools for use in optogenetics research
Optogenetic actuators such as Channelrhodopsin-2 (ChR2) are seven-transmembrane proteins that function as light-gated ion channels. These naturally occurring proteins are found in green algae and serve as sensory photoreceptors controlling phototaxis. Operationally, they contain the …
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Flexible fibers for optoelectronic probing of spinal cord circuits
… the light sensitive protein channelrhodopsin 2 (ChR2). Furthermore, it is shown that the optical stimulation of the spinal cord with the polymer fiber probes induces on-demand limb movements. Finally, the modest dimensions and high flexibility of the devices permitting chronic implantation into …
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Strategies for optogenetic stimulation of deep tissue peripheral nerves
… to the clinic with a human trial using the opsin ChR2 in the treatment of retinitis pigmentosa currently underway and several more trials potentially planned for the near future. In this thesis, we focus on the use of viral techniques to transduce peripheral nerve tissue to be responsive to light. …
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Novel approaches to explore mechanisms of epileptic seizures - optogenetic and chemogenetic manipulation of hippocampal circuitry
… mice expressing the excitatory cation channel ChR2. Additionally, we corroborated the well known role of the dentate gyrus is this type of progressive seizure model. Finally, using the chemogenetic novel hyperpolarizing Gi-DREADD receptor, activated by CNO, we could show that inhibiting the …
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Characterization of spontaneous and evoked activity of mouse embryonic stem cell derived motor neurons using optogenetics and micro electrode array electrophysiology
… line was transfected with channelrhodopsin-2 (ChR2) tagged with Td-Tomato which allowed for optogenetic stimulation of the networks with a 470 nm LED. Network firing patterns were evaluated for bursting activity and spectral content using analysis algorithms developed in MATLAB. Data presented …
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Multisite optical neuromodulation : invention and application to emotion circuits
… light-activated ion channels Channelrhodopsin-2 (ChR2) and Halorhodopsin (Halo) could be used to treat circuit pathologies because they enable bidirectional control of transfected neurons with high temporal and spatial resolution. Virally delivered to mammalian neurons and expressed under …
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Octopaminergic Regulation of Olfactory Discrimination in the Drosophila Larval Mushroom Body Calyx
… functionality of anatomical circuits, I used ChR2-XXL to activate the calyx-innervating inhibitory neuron APL, and recorded Ca2+ responses in KCs. I then applied this strategy to activate OA neurons, and observe the responses in projection neuron (PN) terminals or KCs. I found a tendency …