Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 13 of 13 for “"Centriole"”.
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A screen for novel interactions within the vertebrate centriole
The centrioles organize the centrosomes and cilia which play a role in cell division, motility, sensing, polarity, trafficking and signalling. There has been an increase in the understanding of centriole composition and function recently. However, the exact assembly mechanism of the centriole at …
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Structural and Functional Analysis of the Protein Network Controlling Centriole Biogenesis
… cilia and flagella formation. The duplication of centrioles, which are at the core of each centrosome, is tightly regulated and occurs only once per cell cycle, which allows for correct segregation of chromosomes. In <i>C. elegans</i>, only five proteins are essential for centriole duplication. …
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Role of the Rcd4:Ana3 sub-complex in Drosophila centriole duplication
… framework of conserved proteins that govern the centriole duplication cycle. In an attempt to search for additional molecular components, two genome-wide RNA interference screens were conducted in cultured Drosophila cells (Dobbelaere et al., 2008; Goshima et al., 2007). While these studies …
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The dual use of cohesin and its protector Sgo1 contributes to the choreography of the chromosome and the centrosome cycle
… licensing step in late mitosis/early G1 phase, centriole disengagement. Reminiscent of the control of chromosome number, this dependence usually ensures that centrosomes are duplicated only once per cell cycle. The multi-subunit protein complex cohesin forms a tripartite Scc1-Smc1-Smc3-ring …
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Stiffness controls primary cilia position and length: mechanisms and potential in reversing osteoarthritis phenotypes
… stiffness on cilia frequency, length and centriole position as well as cell and nuclei area over time in mouse articular chondrocytes. Contrary to other cell types, this study shows that chondrocyte primary cilia shorten on softer substrates demonstrating tissue-specific mechanosensing …
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Investigating the cytoplasmic role of E2F4 in multiciliogenesis
… in the nucleus to transcriptionally activate centriole biogenesis genes required for cilia formation. Subsequently, E2F4 locates to the cytoplasm and colocalizes with the early components of deuterosome complexes, which enable the large-scale amplification of basal bodies, from which cilia are …
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INVESTIGATION OF THE DISTAL APPENDAGE PROTEIN CEP164 IN Trypanosoma brucei
… plasma membrane and are structurally similar to centrioles. Docking of the basal body requires the presence of transitional fibres, which mature from distal appendages on the centriole. These structures rely on the presence of the Cep164 protein to carry out docking and ciliogenesis. …
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Towards structural characterisation of ciliary rootlets, ciliary caps and intraflagellar transport
… and the ciliary caps. In interphase cells, centriole-pairs are connected by striated fibrils composed of the coiled-coil protein rootletin along with CEP68, CCDC102B and potentially other proteins. Upon ciliogenesis, the centriole-pairs template the ciliary cytoskeleton and the fibrils merge …
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Characterisation of ALADIN’s function during cell division
… were also found at the base of the cilia, a centriole-derived organelle that participates in differentiation, migration, cell growth from development to adulthood. Here it is shown that ALADIN is also localised at the base of the cilia. With this work, new ALADIN’s functions have been …
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Functional Elucidation and Characterization of GPA2/GPB5 and Its Receptor LGR1 in the Mosquito, Aedes Aegypti
… localized to the plasma membrane of the centriole adjunct, which contains proteins important for flagellar formation in developing spermatids. Using RNA interference, downregulation of LGR1 led to sperm defects, such as shortened flagella. Moreover, newly emerged males were characterized …
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Human cohesin dynamics and their regulation in M and S phase
… the centrosomes. Each centrosomes comprises two centrioles, which are rigidly coordinated ("engaged") in a perpendicular fashion. Separase-mediated proteolysis of centrosome- associated cohesin causes centriole disengagement, a prerequisite for centrosome duplication in the following S phase. In …
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Investigating the structure and function of HSV-1 tegument proteins: UL7 and UL51
… between UL51 and the G-Box domain of the centriole protein CPAP was identified by Y2H screen, and validated by immunoprecipitation from transfected cells and in pull-down experiments using recombinant proteins purified from E. coli. The CPAP-binding region within UL51 was mapped and shown …
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Towards the understanding of pericentriolar satellite biology
Pericentriolar satellites (PS) are electron dense granules surrounding the centrosome, the major microtubule-organizing centre in eukaryotic cells. In cycling cells the centrosome promotes spindle assembly and the faithful execution of mitosis. In non-cycling cells it is involved in forming the …