Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 29 for “"Central Dogma"”.
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Targeting the Central Dogma to Inhibit Nodes of Convergence in Cancer
… These nodes can be found within the central dogma processes that drive all cellular function. In this dissertation, this strategy is used to discover inhibitors of nodes involved in transcription, translation, and protein folding of oncogenes and oncoproteins. Targeting nodes of …
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A Common Cellular Response to Broad Splicing Perturbations
… responses to disruptions in many steps of the central dogma - from DNA synthesis to post-translational protein processing - are well classified. However, we have yet to establish a unifying mechanism describing how cells respond to disruptions in the process of mRNA splicing. Here, I …
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How Chromosome-Nuclear Envelope Attachments Affect 3D Genome Organization
… and is reshaping the traditional concept of the central dogma. Although little is known about the principles which govern chromosome folding and influence gene regulation, the nuclear envelope is expected to play a significant role since it serves as the physical boundary preventing chromosome …
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Developing Bottom-Up, Integrated Omics Methodologies for Big Data Biomarker Discovery
… biologists assumed a strict adherence to the central dogma: replication, transcription and translation. Recent studies in genomics and proteomics emphasize that this is not the case. We must employ big-data methodologies to not only understand the biogenesis of these molecules, but also their …
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The synthesis and in vitro evaluation of short interfering RNAs that contain internal modified alkyl spacers
… ability to silence genes at this point in the central dogma of cell biology offers the chance for excellent gene knockdown specificity as the gene silencing tool is tailored for an exact messenger RNA sequence. There are some inherent problems associated with this type of technology which arise …
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Multi-color visualization and quantification of single RNA translation and HIV-1 programmed ribosomal frameshifting in living cells
Sixty years ago, Francis Crick first stated the central dogma of molecular biology: DNA makes RNA, RNA makes protein. At that time the central dogma could only be imagined, but over the past two decades revolutionary advances in fluorescence microscopy have now made it possible to directly image …
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Targeting RNA Structures with Multivalent Branched Peptide Libraries
… for the transfer of genetic information, as the central dogma of biology dictates. The role of RNA, however, is not limited to serving as an information shuttle between DNA and fully functional protein. Indeed, RNA has experienced a surge of interest in the field of chemical biology for its other …
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Accessible and easy-to-use educational tools to teach molecular and synthetic biology using freeze-dried, cell-free technology
… modules using this platform that can teach the central dogma of biology, how certain cellular functions work, and other basic molecular biology topics that are otherwise difficult to easily teach in a hands-on manner. By expanding the platform to other non-visual outputs (such as smell or …
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Germline Genetic Modulator of Cancer Metastasis
… having a profound impact on patient survival. A central dogma in cancer is that during tumorigenesis, cancer cells acquire somatic mutations that regulate metastatic likelihood during evolution of the primary tumor. However, despite extensive tumor sequencing efforts, such causal somatic …
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The elucidation of immunological and oncological transcriptomic signatures using translational ontologies and next-generation sequencing
… these oncological and immunological analyses, a “central dogma” of transcriptomic expression patterns can be derived. In chapter 5, key conclusions and future directions will be discussed in more detail, and the translational implications of the pipeline and ontology explored.
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LncRNA discovery in the Listeria monocytogenes infection model
… have since discredited the long standing central dogma formulated by Franscis Crick in 1958, which states that genetic output is entirely conducted by protein. Recent studies collectively indicate that lncRNAs play important functional roles in the transcriptional regulation of a wide …
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Assembling The Building Blocks For A Unified Splicing Code
… for the functioning of biological systems. The central dogma of molecular biology describes the processing of this code: DNA is transcribed to RNA, which is translated to proteins. In eukaryotes, DNA is transcribed to pre-mRNA. Subsequently, a single pre-mRNA can encode for different proteins by …
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Modeling Disorder in Proteins Yields Insights into the Evolution of Stability and Function
<p>The central dogma of molecular biology dictates that a DNA sequence codes for an RNA sequence, which in turn codes for a sequence of amino acids that comprises a protein. Proteins are responsible with performing myriad functions within living organisms and most proteins require a folded …
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The Sequence Landscape of Bacterial Genes is Shaped by Long-Range mRNA Folding
… tertiary topologies that the molecules of the central dogma can fold into. Specifically, though local messenger RNA (mRNA) structures are known to regulate bacterial gene expression, the role of long-range mRNA folding remains unclear despite the predicted prevalence of such interactions across …
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Genome-Scale Quantification of Target Vulnerability in Mycobacterium Tuberculosis
… We define highly vulnerable steps in Mtb central dogma processes, protein secretion and cell wall synthesis, including targets currently unexplored in the drug discovery pipeline. Likewise, we identify invulnerable processes that can withstand substantial inhibition with little impact on …
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Plutonium pharmacokinetics and blood biochemistry
… are based on such broad understanding and a "Central Dogma" has arisen viz, plutonium is bound avidly in liver and bone; clearance half-lives from these organs differ (by a factor of 2.5) but are very long - a minimum of 50 years for bone; this is why plutonium urinary excretion levels are …
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Single-Molecule Protein Sequencing (I) and Genetically Dominant mRNA Therapies to Combat Viral Evolution (II)
… for quantification and identification. The central dogma of molecular biology, information flow from DNA to RNA to protein, has been studied for decades as these molecules are critical to cell function and diversity. The advent of polymerase chain reaction (PCR) amplification of nucleic acid …
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Studies of Entry, Reverse Transcription, and Regulation of Splicing in Retroviruses
… of the world of biology and medicine. The central dogma of the basic flow of genetic information was shattered when the discovery that retroviruses copy their RNA genome into DNA was made. The same enzyme that performs this step, reverse transcriptase (RT), also revolutionized molecular …
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The role of sphingosine kinases and dihydroceramide desaturase in regulating senescence in prostate cancer cells
… treatment. In addition, the study challenged a central dogma in the field and provided useful and previously unknown mechanistic information about a drug which is currently in phase 2 clinical trials. Finally, the study identified a common novel target (Des1) of two existing compounds which, in …
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The Proteomics Approach To Evolutionary Computation: An Analysis Of Pr
… functions or designs. The observation from the central dogma of molecular biology that proteins are the basic building blocks of life and the recent advances in proteomics on analysis of structure, function and interaction of entire protein complements, lead us to propose a unifying framework of …
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