Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 10 of 10 for “"Cellulose-binding domain"”.
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Production and preliminary characterization of a fusion protein comprising streptavidin and a cellulose-binding domain
The cellulose-binding domain of exoglucanase Cex (CBDCex) from Cellulomonas fimi can be fused to heterologous proteins. The fusion proteins can be purified by affinity chromatography on cellulose or immobilized on cellulose (Ong et al., 1989b). Streptavidin, a protein produced by Streptomyces …
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Use of the cellulose-binding domain of a cellulase from cellulomonas fimi for affinity purification of fusion preteins
This study describes the use of a cellulose-binding domain (CBD) from a bacterial cellulase as an affinity tag for purification of heterologous proteins. The CBD of endoglucanase A (CenA) from Cellulomonas fimi is an N-terminal domain comprising amino acids. CenA binds strongly to cellulose, and …
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Molecular Analysis of a Cellulase/xylanase gene,celD, From the Ruminal Bacterium Ruminococcus Flavefaciens Fd-1
… against both Ostazin brilliant red-hydroxyethyl cellulose and carboxymethyl cellulose (CMC) was isolated. This clone (designated FD1-1) was further analyzed by restriction endonuclease mapping and Southern blot analysis. Substrate specificity data show that the cloned gene encodes both …
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Characterization of endoglucanase cela from the rumen bacterium Clostridium longisporum
Cellulose is the most abundant organic compound on earth and offers great potential as a source of renewable energy and other chemicals. Cellulases are being studied to elucidate the enzymatic degradation of cellulose. We are interested in the molecular mechanisms of microbial degradation of …
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Development of a regenerable glucose biosensor probe for bioprocess monitoring
… immobilization of enzymes conjugated with the cellulose binding domain (CBD)of cellulases from Cellulomonas fimi. A regenerable biosensor probe is configured with a cellulose matrix onto which the solubilized enzyme-CBD conjugate can be repeatedly loaded (via the attachment of the CBD) and …
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Identification and Characterization of In-planta Expressed Secreted Effector Proteins from Magnaporthe oryzae
… MGG_08370, MGG_08409, contain carbohydrate-binding modules (CBMs). To elucidate the pathogenesis function of the three CBM-containing effectors, we generated transgenic M. oryzae strains that carried out gene replacement and gene overexpression. Among the three of them, the MGG_08409 …
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Printing protein-engineered paper for biosensors
… with the recombinant protein production of paper-binding bioreagents could provide an accessible, scalable tool for the fabrication of affordable paper-based microfluidic biosensors with customised and varying paper properties. A novel printing method based on the localised vacuum-driven …
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Biomass pretreatment toward efficient hydrolysis for sustainable biofuel applications
… been investigated to understand their impacts on cellulose and/or lignocellulose and the subsequent hydrolysis steps. Physicochemical pretreatments, such as steam explosion, have been identified as one of the most effective and cost-efficient pretreatment methods for lignocelluosic materials. In …
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Understanding the molecular basis of Zymoseptoria tritici-wheat interactions
… disease symptoms inplanta. Zt61 has a predicted cellulose binding domain and shares high structure similarity to bacterial expansin protein, BsEXLX1. Hence, Zt61 gene was cloned and expressed in E.coli, to purify Zt61 protein, further confirmed via mass spectrometry. Upon wheat infiltration, it …
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Understanding the molecular basis of Zymoseptoria tritici-wheat interactions
… disease symptoms inplanta. Zt61 has a predicted cellulose binding domain and shares high structure similarity to bacterial expansin protein, BsEXLX1. Hence, Zt61 gene was cloned and expressed in E.coli, to purify Zt61 protein, further confirmed via mass spectrometry. Upon wheat infiltration, it …