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Showing 1 to 12 of 12 for “"Cellulose biosynthesis"”.
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Characterization of a novel cellulose biosynthesis inhibitor, CBI28, in Gluconacetobacter xylinus
To study the underlying mechanisms for microbial cellulose biosythesis, a novel compound, CBI28, was used as an inhibitor along with classical genetics and EMS mutagenesis. An EZ-Link Biotin Hydrazide Kit was used to create a CBI28-Biotin conjugate for further studies. Gluconacetobacter xylinus …
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Characterization and transcriptomic profiling of Arabidopsis thaliana under cellulose biosynthesis inhibitor treatment
Cellulose is one of the most important components of plant cell walls for industry and plant development. Screening mutants resistant to chemicals that conditionally inhibit cellulose synthesis, known as cellulose biosynthesis inhibitors (CBIs), allows us to dissect the cellulose biosynthetic …
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Forward genetic analysis of cellulose biosynthesis inhibitor resistance and wall hydrolysis sensitivity.
The functional analysis of components involved in cellulose biosynthesis is central in understanding cell wall assembly and structure in plants. We conducted screens using the herbicides, isoxaben and flupoxam which inhibit cellulose biosynthesis in higher plants. Mutations resulting in a high …
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Forward and reverse genetic approaches to investigate cellulose biosynthesis in Physcomitrium patens
Cellulose biosynthesis is a common feature of land plants and involves multimeric complexes composed of cellulose synthase A (CESA) proteins and other structural proteins. The exact stoichiometry of CESA proteins and interactions among proteins within cellulose synthase complex (CSC) is not well …
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Cellulose biosynthesis inhibitors modulate defense transcripts and regulate genes that are implicated in cell wall re-structuring in arabidopsis
… step in cell wall synthesis pathway using Cellulose Biosynthesis Inhibitors (CBIs). In this case, chemical screen for swollen organ phenotype has proved to be an important technique to identify the genes that are directly or indirectly involved in cellulose biosynthesis. In the present …
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Ethylene response and phytohormone-mediated regulation of gene expression in Komagataeibacter xylinus ATCC 53582
… xylinus ATCC 53582 is a fruit-associated, cellulose-producing bacterium that responds to and synthesizes phytohormones. This thesis elaborates on the ecophysiology of K. xylinus. Responses to indole-3-acetic acid (IAA), abscisic acid (ABA) and ethylene, produced in situ from ethephon, were …
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Characterization of novel genetic alleles of cellulose synthase
Cellulose is the most abundant polymer on earth. Despite this, relatively little is known about the proteins and biochemical mechanisms governing cellulose biosynthesis. Only in the past 10 years since the sequencing of the Arabidopsis thaliana genome have we begun to unravel the full complexity of …
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Forward screening for herbicide resistance in Arabidopsis thaliana and the monocot species Triticum aestivum
Cellulose is the primary structural component of plant cell walls and is composed of β-1,4-glucan chains. The cellulose synthesis complex is composed of multiple cellulose synthases (CESAs), which work together to extend the cellulose molecule. Although extensively studied, cellulose biosynthesis …
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The transcription factor, fixK, plays a central role in carbon source metabolism and cellulose synthesis in Komagataeibacter species
… and K. hansenii ATCC 23769 are model bacterial cellulose producers. This thesis investigated both carbon source utilization and the role that a Crp/Fnr transcription factor, FixK, plays in bacterial cellulose biosynthesis. Extracellular proteins secreted by Komagataeibacter were assayed for …
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The Cloning and Characterization of Two ROP/RAC G-Proteins from Gossypium Hirsutum
… fiber elongation, decreasing significantly when cellulose biosynthesis began. Transcript abundance of GhRac3 doubled between fiber elongation and secondary wall synthesis, remaining constant until 20 days post-anthesis. Expression of GhRac2 and GhRac3 was compared between the unfertilized ovules …
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THE SUBTLE BIOFILM REGULATION IN ESCHERICHIA COLI: CSGD AND THE YDDV-DOS OPERON
… signals and on regulation of curli fibers, cellulose and poly-N-acetylglucosamine (PNAG), the most important biofilm determinants in E. coli. E. coli is an Enterobacterium, normally living inside the mammalian gut, at temperature of 37° C and in relatively nutrient-rich environment. Once …
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Structural colour from a helicoidal cellulose architecture in the secondary cell wall: Optical properties, cell wall composition, structure and morphology of components, and their assembly and interactions.
… structural coloration consists of assembling cellulose microfibrils into helicoidal structures. Examples of these architectures can be found in phylogenetically distant species and in different tissues of plants, such as in the endocarp of the fruit of $\textit{Margaritaria nobilis}$, an …