Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Cellular oxygen"”.
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New phosphorescence based probes and techniques for the analysis of cellular oxygen and respiration
Real time monitoring of oxygenation and respiration is on the cutting edge of bioanalysis, including studies of cell metabolism, bioenergetics, mitochondrial function and drug toxicity. This thesis presents the development and evaluation of new luminescent probes and techniques for intracellular O2 …
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Development of EPR Methods for Simultaneous Measurements of Intra- and Extra-Cellular Oxygen Concentration in Vitro
… for simultaneous measurement of intra- and extracellular concentration of oxygen ( (O$\sb2$)) in viable cells have been developed.
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Defect of Mitochondrial Translation Machinery Enhances Hepatoma Cell Invasiveness
… chain reaction (RT-PCR) 4 3. Endogenous cellular oxygen consumption rate 5 4. Estimation of intracellular and mitochondrial ROS level 5 5. Total genomic DNA isolation and sequencing of mitochondrial DNA fragments 5 6. Western blot analysis 6 7. Real-time reverse transcription-polymerase …
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Interactions Between Heme-Globins and Ligands
… detoxifying reactive species (O2 and NO), cellular oxygen homeostasis, and reversible binding of O2 with a higher binding affinity than hemoglobin.6 Tejero et al. previously showed that a mutant form of Ngb reduces nitrite to nitric oxide 50x faster than myoglobin and 500x faster than …
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Design, modeling, and validation of an apical flow Transwell insert for small intestinal models
… velocity profiles of media flow as well as oxygen transport and cellular oxygen consumption are modeled. External to the device, heat transfer from a warm incubator into room temperature tubing containing media is modeled to ensure proper media warming. Experimental validation of the flow …
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Regulation of mitochondrial respiration by LDHB suppression in hepatoma cell lines
… was suppressed by siRNA-mediated knockdown, cellular oxygen consumption rate was significantly decreased without clear decrease in expressions of respiratory proteins. These results imply that LDHB suppression may control mitochondrial respiration through post translational modification or …
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VHL inhibitors as chemical probes of the hypoxia signalling pathway
… function of VHL in response to hypoxia and cellular oxygen sensing are well established, owing to the use of genetic tools through knockout and knockdown that inactivate VHL. However, the functional consequences of specifically interrupting the interaction between VHL and HIF α remain to be …
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The role of ABHD11 in the regulation of the hypoxia inducible transcription factors
… factors (HIFs) mediate a highly conserved cellular oxygen sensing system, driving a diverse set of transcriptional changes that allow adaptation to hypoxic conditions. However, HIFs can also be activated independently of oxygen by changes in levels of mitochondrial metabolites, a mechanism …
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Studies on renal progenitor cells and kidney cancer
… process is still not fully elucidated and the cellular origin for regeneration is extensively debated. Kidney cancer is characterized by vague clinical symptoms and pronounced resistance to common cancer therapies, like chemotherapy and radiation. Consequently, nearly 25% of patients present …
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Investigating the role of mitochondria in the hypoxia response using in vitro and in vivo models
… CHCHD4 is critical for maintaining intracellular oxygenation and metabolism in human cells, partly by controlling the expression of respiratory chain complex subunits. In cancer, CHCHD4 is required for tumour growth in vivo, with overexpression in a range of cancers correlating with …
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Mitochondrial Uncouplers: Development as Therapeutics for Metabolic Diseases
… with this novel scaffold that increased cellular metabolic rates in vitro using changes in oxygen consumption rate as a read-out. In particular, compound SHS4121705 (2.12i) displayed an EC50 of 4.3 M in L6 myoblast cells and excellent oral bioavailability and liver exposure in mice. In …