Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 11 of 11 for “"Cellular forces"”.
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Spatial Dynamics and the Mechanoresponse in CD4+ T Cell Activation
… in generating TCR-driven contractile forces is then investigated through use of micropillar arrays. This approach also enables the quantification of forces generated by T cells during cellular activation. The impact of CD28 costimulation on TCR-driven force generation is assessed and …
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Mechanical Manipulation of Eukaryotic Chromatin by DNA-Binding Proteins
… 1) protein:DNA co-condensation can generate high forces, up to ~7 pN, comparable to other reported cellular forces, 2) the intrinsically disordered regions (IDRs) are dispensable for condensate formation but necessary for high force generation, and lastly, 3) chromatin components, such as …
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Cells and force transduction
… how force and substrate stiffness guide cellular decision-making during initial cell attachment and spreading on deformable substrates. The well-established dependence of cell traction and spreading on substrate stiffness has been attributed to levels of force exerted on molecular …
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A new computational model for multi-cellular biological systems
… the mechanical properties of a cell and inter-cellular forces affect the movements we see within a tissue. It is designed to probe the relationships between cellular and tissue behaviours; such as how forces propagate through a system or the response of a tissue to applied forces. The novel …
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Piezo1-dependent mechanical regulation of chemical signalling in the developing Xenopus laevis brain
… signals include tissue stiffness, viscosity and cellular forces. In the developing embryo, chemical and mechanical signals are not isolated. However, how cells integrate these two different types of signals remains poorly understood. Here, I investigated how tissue stiffness and longrange …
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Cell-mediated contraction & induced regeneration of the injured peripheral nerve
Cell-mediated mechanical forces drive closure of severe wounds in adult mammalian organs, including the sciatic nerve following neurotmesis. Without experimental intervention the defect closes rapidly via contraction of transected nerve stumps by a thick, cohesive capsule of myofibroblasts (MFB) …
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Engineering Substrates for the Study of Cell Mechanical Interactions
… with controlled radius of curvature on cellular behavior, a novel approach to measure and map protrusive forces in cells and the application of fabrication techniques like wet etching and microcontact printing to answer fundamental biological problems. In order to isolate the effects of …
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Cytoskeletal Remodeling in Fibrous Environments to Study Pathophysiology
… of cells with their immediately surrounding extracellular matrix (ECM) is now known to be critical in pathophysiology. For example, during cancer progression, while uncontrollable cell division leads to tumor formation, the subsequent metastatic migration of cells from the primary tumor site to …
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Traps, Tensions and Transmissions: The Unionisation of Resistance Movements against External Destabilising Forces; Design, Development and Deployment of Holographic System for Active Surveillance and Targeted Manipulation
… the dynamics of molecules, measure local forces, and re-localise or switch molecular behaviour. Here, we present the first application of this hybrid system to the study of cell shape regulation and the role of effective membrane tension in unionising actomyosin movement to resist external …
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Emergent behavior of cells on microfabricated soft polymeric substrates
… dynamic deformation of the substrates on the cellular and cluster level organization of the cells remains elusive. This dissertation tries to explore the emergent behavior of cells on different types of micro-scale deformable, soft polymeric substrates. In the first part of the dissertation, …
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A Nuclear Mechanoresponse to Morphogenesis Drives Acquisition of Neuroectoderm Lineage Competence During Pluripotent Cell Differentiation
… morphogenesis-associated, actomyosin-generated cellular forces to the nucleus and influence cell state. Nuclear mechanosensitivity can result in changes a cell’s molecular-epigenetic state and regulate gene expression during differentiation, thereby influencing cell fate. During implantation, …