Global ETD Search
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Showing 1 to 20 of 29 for “"Cells--Analysis"”.
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Regulation of progesterone receptor expression and activity in uterine cells: Analysis of the molecular actions of estrogens and progestins on the progesterone receptor gene
Estrogens and progestins play important roles in many aspects of female reproductive biology. These hormones exert their effects via intracellular protein receptors, termed estrogen receptors (ERs) and progestin receptors (PRs). We have examined the regulation of PR levels in the rat uterus by …
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Topology and analysis in power conversion and inversion
… as a converter cell, is identified. Converter cells can be used in generation and classification of basic PWM dc-to-dc converters. A large number of new converters are generated. Converter analysis, whereby the nonlinear response of the system to perturbations in the control or the input, is …
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The construction and characterization of recombinant sindbis virus vectors as a platform for anthrax vaccine development
… and promoting toxin entry into susceptible cells. Analysis of the PA molecule has identified a specific region, termed domain four, that contains epitopes responsible for antibody binding. Sindbis virus, the prototypical member of the genus alphavirus, has been extensively used as a vector …
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Investigating The Role of The P63 Isoform Δnp63 In Lung Stem Cell Populations and Lung Cancer
… separate populations of lung progenitor cells that give rise to lung adenocarcinoma and squamous cell carcinoma. ΔNp63 is known to regulate lung development but its role in lung cancer progression remains unclear. We utilized a ΔNp63-specific conditional knockout mouse model to determine …
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Design and analysis of MEMS-based metamaterials
… describe a metamaterial constructed of MEMS unit cells. Analysis of these models provides information about the potential behavior of a MEMS metamaterial. It is discovered that it is possible to create a left-handed metamaterial using MEMS resonators. Finally, phase-shifting and antenna …
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Development and Evaluation of an Isospora Suis Sporozoite Neutralizing Antibody Assay in Swine Testicular Cells
… has been developed in swine testicular (ST) cells. This assay measures the titer of neutralizing antibodies present in sera, milk and monoclonal antibody culture fluids by determining both the amount of intracellular sporozoites by immunofluorescence and the cytopathic effect in ST cells. A …
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An ancestral regulatory mechanism underlies Hoxd gene expression in both developing genitals and digits
… as forming a regulatory archipelago in digit cells. Analysis of the epigenetic status and spatial conformation of the HoxD cluster and its centromeric neighborhood has identified several putative regulatory elements that interact with Hoxd genes via chromatin looping in the developing genital …
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Modelling metabolic landscapes in human naive and primed embryonic stem cells
… this transition can be modelled with pluripotent cells in culture which exist in a naive (more pluripotent) and a primed (epiblast like) state and show the same differences in metabolic regulation. These two cell states also show major differences in their epigenetic landscape including in DNA …
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Multi-Constriction Microfluidic Sensors for Single-Cell Biophysical Characterization
… easy-to-use technologies capable of classifying cells. Analysis of cell behavior in microfluidic deformability assays provides a label-free method of observing cell response to physical and chemical stimuli. This body of work shows advancements made toward reaching our goal of a robust and …
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Invadopodia Formation in Breast Cancer
… To resist the negative effect of hypoxia, cells increase expression of transcription factor hypoxia-inducible-factor-1 (HIF-1α). However, little is known about how HIF-1α might influence the invasive behaviour of the cancer cells. Cell invasion requires actin cytoskeletal reorganisation and …
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An investigation into dystroglycan deficiency in fibroblasts and a role for dystroglycan in cytokinesis
… from dystroglycan null embryonic stem cells. Analysis of these cells by western blot, however, showed them to be expressing dystroglycan and this was confirmed by RT-PCR, genomic PCR and northern blot. Subsequently, Swiss 3T3 fibroblasts with greater than 60% reduction in dystroglycan …
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Correlation of Heparan Sulfate Proteoglycan Structure and Metabolism With the Regulation of Cell Division
… a ubiquitous cell surface component in animal cells, is a complex, polyanionic macromolecule consisting of a core protein to which are attached several heparan sulfate (HS) chains. Growing and confluent cultures of a rat hepatocyte cell line were labeled with $\sp{35}$SO$\sb4\sp{2-}$ and the HS …
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Development and application of tools for glycan characterization
… and fetuin were successfully profiled, and the analysis of the former provided the motivation for a second project focused on the significance of goblet cells and mucins in influenza infection. This project explored the potential benefits of a glycoprotein direct binding assay as a way to obtain …
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Targeting iNAMPT to Break the Obesity-Associated Liver Cancer Link: An In Vitro and In Vivo Approach
… focused on inhibiting its behavior in cancer cells. The objective of this study was to determine the contribution of NAD biosynthesis in obesity-induced liver cancer progression. Methods: Cell studies were conducted with serum from mice randomized to a diet-induced obesity (OB) or control chow …
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Regulation of The Oxidative Stress Response By Arid1A
… Sensitivities in Cancer’ database. From this analysis, I found that <em>ARID1A</em>-mutant cell lines are more sensitive to elesclomol, which is a reactive oxygen species (ROS)-inducing agent. This finding was validated using a panel of ovarian and endometrial cancer cell lines, where …
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Characterization of The Ato Gene Family In Alternative Carbon Metabolism
… Macrophages are important innate immune cells that limit the niches in the human body in which <em>C. albicans </em>can persist through phagocytic removal. However, following phagocytosis <em>C. albicans</em> readily escapes from the immune cell by differentiating into filamentous hyphae, …
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Conserved and Novel Properties of Clathrin-Mediated Endocytosis in Dictyostelium Discoideum
… that is homologous to that present in mammalian cells. Analysis of this pathway at a high spatial and temporal resolution shows the high degree of similarity in the kinetics of internalization of individual clathrin-coated vesicles between Dictyostelium and mammalian cells. These similarities …
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Mechanisms of dynein-based mRNA transport processes in human cells
Protein function in cells is intricately regulated in both space and time through the positioning of mRNA molecules in the cytoplasm. This process is commonly mediated by molecular motors, which transport mRNAs along the cytoskeleton to specific cellular regions. Despite the widespread occurrence …
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Characterizing Chromosomal Aberrations in Cells Deficient for Both ATM and MSH2
… were examined for chromosomal aberrations in B cells, which undergo genetically programmed DNA recombination and mutation. To bypass the lethality in <em>ATM<sup>-/-</sup>MSH2<sup>-/-</sup> </em>mice, a conditional knockout of ATM was bred onto an <em>MSH2<sup>-/-</sup></em> background strain to …
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DEPDC5 is a metabolic checkpoint regulator in the B cell AA-mTORC1 pathway
… and induction of immune responses. Naïve B cells rest in quiescent states until antigen (Ag) is encountered, which simultaneously triggers a metabolic switch enabling enhanced growth, proliferation and differentiation in activated B cells. Central to this metabolic activation is the …
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