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Showing 1 to 8 of 8 for “"Cellobiose Utilization"”.
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Pathway optimization and engineering for biofuel production
… chemicals. One such significant pathway is the cellobiose utilization pathway, identified as a promising route for efficient biomass utilization. Here we describe the simultaneous optimization of the β-glucosidase (gh1-1) and the cellodextrin transporter (cdt-1) through directed evolution of the …
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Strain engineering and biosensor development for efficient biofuel production by Saccharomyces cerevisiae
… Great efforts have been made to improve the co-utilization efficiency of sugars derived from lignocellulose hydrolysates. A lot of research has been carried out to lower the effect of glucose repression that leads to inefficient pentose sugars utilization in the presence of glucose, but it …
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Metabolic basis for the preferential utilization of disaccharide, by the cellulose-decomposing bacterium, cellvibrio gilvus (nov. sp.)
… Cellulose decomposition was demonstrated and cellobiose was the only hydrolytic sugar product. Excellent growth was obtained on mineral salts medium containing cellobiose, a vitamin mixture, and organic nitrogen (casein hydrolyzate) which was required for growth. Volatile and non-volatile …
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Combinatorial biosynthetic pathway engineering for microbial production of biofuels
… strategies for the optimization of the cellobiose utilization pathway, a pathway which is important in biofuel production. One strategy focused on optimizing enzyme combinations by creating a library of homologous proteins from the pathway. A second strategy investigated engineering all …
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The effects of the expression levels of cellobiose transporter and β-Glucosidase on ethanol fermentation
Recently, cofermentation of cellobiose and xylose in yeasts has been reported. It is considered as one of the most innovative strategies to enhance bioethanol production from lignocellulosic fermentation. Through cofermentation with cellobiose and xylose, it is achieved to utilize most abundant two …
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Metabolic engineering of yeast strains for renewable biomass utilization and valuable chemical production
… derived from lignocellulosic biomass, mainly cellobiose and xylose, cannot be assimilated by the industrial microorganisms, such as yeast Saccharomyces cerevisiae. To utilize cellobiose or xylose for the fuels and chemicals production by S. cerevisiae strains, heterologous expression of …
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Improving advanced biofuels production in Saccharomyces cerevisiae via protein engineering and synthetic biology approaches
… of cellulosic biofuels, such as the sequential utilization of sugar mixtures caused by glucose repression and the low efficiency of synthesizing fatty acid derived advanced biofuels. In this thesis, we aim to improve the production of advanced biofuels in S. cerevisiae using protein engineering …
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Design of a Saccharomyces cerevisiae strain capable of simultaneously utilizing cellobiose and xylose
… hydrolysates inhibits xylose and other sugars’ utilization. As a result, it is attractive to construct a glucose derepressed S. cerevisiae strain for efficient utilization of lignocellulosic sugars. In this thesis, we proposed and constructed an artificial cellobiose assimilating pathway …