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Showing 1 to 20 of 62 for “"Cellobiose"”.
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2,3-butanediol production from cellobiose by engineered Saccharomyces cerevisiae
… cerevisiae to produce 2,3-BDO, especially from cellobiose which is a prevalent sugar in cellulosic hydrolyzates. Specifically, we overexpressed alsS and alsD from Bacillus subtilis to convert pyruvate to 2,3-BDO via α-acetolactate and acetoin in engineered S. cerevisiae capable of fermenting …
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Design of a Saccharomyces cerevisiae strain capable of simultaneously utilizing cellobiose and xylose
… we proposed and constructed an artificial cellobiose assimilating pathway consisting of a cellobiose transporter and a β-glucosidase in S. cerevisiae. A total of six different cellobiose assimilating pathways were constructed and compared in a laboratory S. cerevisiae strain capable of …
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A computational study of acidic Ionic Liquids for cellobiose hydrolysis in ionic liquids
… the β-(1,4)-glycosidic bond linkages in the cellobiose polymer repeating units are cleaved. Problems arise in the hydrolysis of cellulose as experimental and theoretical studies have shown cellulose to have very low solubility in water and most other general molecular solvents. This results …
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The effects of the expression levels of cellobiose transporter and β-Glucosidase on ethanol fermentation
Recently, cofermentation of cellobiose and xylose in yeasts has been reported. It is considered as one of the most innovative strategies to enhance bioethanol production from lignocellulosic fermentation. Through cofermentation with cellobiose and xylose, it is achieved to utilize most abundant two …
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Metabolic basis for the preferential utilization of disaccharide, by the cellulose-decomposing bacterium, cellvibrio gilvus (nov. sp.)
… Cellulose decomposition was demonstrated and cellobiose was the only hydrolytic sugar product. Excellent growth was obtained on mineral salts medium containing cellobiose, a vitamin mixture, and organic nitrogen (casein hydrolyzate) which was required for growth. Volatile and non-volatile …
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Pathway optimization and engineering for biofuel production
… chemicals. One such significant pathway is the cellobiose utilization pathway, identified as a promising route for efficient biomass utilization. Here we describe the simultaneous optimization of the β-glucosidase (gh1-1) and the cellodextrin transporter (cdt-1) through directed evolution of the …
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Improving advanced biofuels production in Saccharomyces cerevisiae via protein engineering and synthetic biology approaches
… approaches. To overcome glucose repression, a cellobiose utilization pathway consisting of a cellodextrin transporter and a β-glucosidase was introduced into S. cerevisiae to allow co-fermentation of mixed sugars. However, the utilization of cellobiose was still much lower than that of glucose, …
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Metabolic engineering of yeast strains for renewable biomass utilization and valuable chemical production
… derived from lignocellulosic biomass, mainly cellobiose and xylose, cannot be assimilated by the industrial microorganisms, such as yeast Saccharomyces cerevisiae. To utilize cellobiose or xylose for the fuels and chemicals production by S. cerevisiae strains, heterologous expression of …
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Molecular ecology of plant cell wall hydrolysis by mixed cultures of Ruminococcus albus 8, Ruminococcus flavefaciens FD-1 and Fibrobacter succinogenes S85
… as monocultures, dicultures and in triculture on cellobiose, cellulose and alkaline hydrogen peroxide-treated wheat straw to study competition among the three bacterial species on these substrates. The cellubiose-grown cultures were incubated at 39$\sp\circ$C for 10 hours and sampled hourly. The …
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Strain engineering and biosensor development for efficient biofuel production by Saccharomyces cerevisiae
… strain capable of simultaneously utilizing cellobiose and xylose derived from lignocellulose. The high efficiency pathway containing a cellobiose transporter and a β-glucosidase enables fast cellobiose utilization and ethanol production, and glucose repression is avoided by the intracellular …
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Cellulolytic enzyme systems of Myrothecium verrucaria
… which synthesized higher saccharides from cellobiose. In paper electrophoresis at pH 8.55 in veronal buffer of ionic strength 0.05 and a potential gradient of 10 volts/cm, concentrated culture filtrates exhibited multiple components with cellulase activity. The electrophoretic pattern for …
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Engineering yeast strains for producing fuels and value-added chemicals from cellulosic biomass
… challenges. Simultaneous co-utilization of cellobiose (a dimer of glucose) and xylose could bypass the inhibition of xylose uptake by glucose and improve ethanol production. To this end, heterologous genes coding for cellodextrin transporter (cdt-1) and β-glucosidase (gh1-1) were integrated …
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Density Functional Theory Calculations Complement Mass Spectrometry Experiments in the Investigation of Biomass Fast Pyrolysis and Ion-Molecule Reaction Mechanisms
… a simple model compound, the cellulose dimer, cellobiose. A novel approach of using mass spectrometry to instantly characterize fast pyrolysis vapors allows for identification of initial products to help delineate the reaction pathways that dictate the final product distribution of fast …
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Combinatorial biosynthetic pathway engineering for microbial production of biofuels
… strategies for the optimization of the cellobiose utilization pathway, a pathway which is important in biofuel production. One strategy focused on optimizing enzyme combinations by creating a library of homologous proteins from the pathway. A second strategy investigated engineering all …
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EVALUATION OF CELLULOLYTIC ENZYMES FROM A NEWLY ISOLATED BREVIBACILLUS SP. JXL; AND OPTIMIZATION OF COSLIF PRETREATMENT VARIABLES OF SWEET SORGHUM BAGASSE USING A RESPONSE SURFACE METHOD
… cellulose, carboxymethylcellulose (CMC), xylan, cellobiose, glucose, and xylose. In two different media supplemented with crystalline cellulose and CMC at 57°C under aeration, strain JXL produced a basal level of cellulases as FPU of 0.02 IU/ml in the crude culture supernatant. When glucose or …
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Engineering and evaluation of yeast strains for the production of lactic acid from cellulosic sugars
… sugars including glucose (6C), xylose (5C) and cellobiose (a glucose dimer). Complete conversion of each of these sugars is necessary to efficiently produce a target fermentation product from lignocellulosic hydrolysates. Until now, biofuels, such as ethanol, have been the only major …
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ΔΙΗΛΕΚΤΡΙΚΗ ΜΕΛΕΤΗ ΤΗΣ ΚΑΤΑΣΤΑΣΗΣ ΤΟΥ ΝΕΡΟΥ ΣΕ ΥΔΑΤΙΚΑ ΔΙΑΛΥΜΑΤΑ ΚΑΙ ΥΔΑΤΩΜΕΝΑ ΣΤΕΡΕΑ ΔΕΙΓΜΑΤΑ ΜΟΝΟ-ΚΑΙ ΔΙ-ΣΑΚΧΑΡΙΤΩΝ
… RIBOSE, AND ARABINOSE AND THE DISACCHARIDES CELLOBIOSE, LACTOSE, AND MALTOSE, IN THE TEMPERATURE REGION OF 80-300K AND CONCENTRATIONS BETWEEN 0 .0003-15 MOL/LT, AND OF THE HYDRATED SOLID SAMPLES OF GALACTOSE AND MALTOSE IN THE RANGE OF HYDRATION H=0.8-20.2% AND THE SAME TEMPERATURE REGION THE …
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Identification and Optimization of the Antagonistic Potential of Native Spinach Microbiota towards Escherichia coli O157:H7
… potential in vitro and on spinach leaves when cellobiose, a carbon source utilized by the antagonists but not E. coli O157:H7, was added. There were larger culturable populations of bacteria on the leaves of savoyed cultivars compared to flat. From the isolated colonies, 47 displayed antagonism …
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Molecular analysis of two cellulase genes from Ruminococcus flavefaciens FD-1 and their transcriptional regulation
… and other cellodextrins to predominantly cellobiose. Thus CelA is a cellodextrinase. It also has high activity against p-nitrophenyl-β-D-cellobioside. A gene, expressing a protein with both carboxymethyl cellulase and xylanase activity, was cloned from R. flavefaciens FD-1 using an E. …
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Characterization of structure and enzymic activity of endo-l, 4-β-D-glucanases purified from Trichoderma viride
… and IV; it is distinguished by low affinity for cellobiose (K<sub>m</sub>=162 compared to K<sub>m</sub>=1.03 and 1.26 mM) and its ten-fold higher activity with cellotriose (V<sub>max</sub>=2.49 compared to V<sub>max</sub>=0.339 and 0.279 µmole minute⁻¹mg protein⁻¹). In addition, Endoglucanase III …
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