Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 26 for “"Cell-fate decision"”.
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Dynamics of Cell Fate Decision Making Between Sporulation and Competence in Bacillus Subtilis
During multipotent differentiation cells must reliably make a cell fate decision under a variety of conditions, yet remain sensitive to changes in extracellular environment. It is unclear how the cells reconcile these seemingly contradictory requirements. To complicate the issue, the cells often …
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The role of polarisation in the first cell fate decision of the mouse embryo
… process in embryonic development is the first cell fate decision, when cells take on distinct lineage identities for the first time. In mammals, this separates embryonic inner cell mass (ICM) from extra-embryonic trophectoderm (TE) during pre- implantation development. In the mouse, the process …
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Single-Cell Image Analysis Enables High-Throughput Phenotypic Drug Screen and Elucidates Cell-Fate Decision Principles
Cellular phenotypes encode information that can be used to infer the external signals cells experience. Here we applied quantitative image analysis to enable a one-pass multi-class phenotypic drug screen. Our combined experimental and computational approach can functionally annotate large compound …
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Single-Cell Image Analysis Enables High-Throughput Phenotypic Drug Screen and Elucidates Cell-Fate Decision Principles
Cellular phenotypes encode information that can be used to infer the external signals cells experience. Here we applied quantitative image analysis to enable a one-pass multi-class phenotypic drug screen. Our combined experimental and computational approach can functionally annotate large compound …
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Theoretical and Computational Studies on the Dynamics and Regulation of Cell Phenotypic Transitions
Cell phenotypic transitions, or cell fate decision making processes, are regulated by complex regulatory networks composed of genes, RNAs, proteins and metabolites. The regulation can take place at the epigenetic, transcriptional, translational, and post-translational levels to name a few. …
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Bioengineering of a TAT-conjugated Peptide to Modulate the Activity of Glycogen Synthase Kinase-3 in Adult and Embryonic Stem Cells
The intracellular delivery of molecules to modulate signaling pathways and gene expression is a powerful approach to control stem cell fate decision. For applications in gene therapy and regenerative medicine, the use of genetic material and viral vectors raise concerns because stem cells persist …
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Mathematical Models of Some Signaling Pathways Regulating Cell Survival and Death
In a multi-cellular organism, cells constantly receive signals on their internal condition and surrounding environment. In response to various signals, cells proliferate, move around or even undergo suicide. The signal-response is controlled by complex molecular machinery, understanding of which is …
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Dynamics of Cell Fate Decisions Mediated by the Interplay of Autophagy and Apoptosis in Cancer Cells: Mathematical Modeling and Experimental Observations
… biological stress response in mammalian cells that is responsible for clearing damaged proteins and organelles from the cytoplasm and recycling their contents via the lysosomal pathway. In cases where the stress is not too severe, autophagy acts as a survival mechanism. In cases of severe …
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Modeling transcriptomic dynamics and epigenomic conservation
… differentiation processes of embryonic stem (ES) cells and embryo development processes. This thesis work centers on two aspects in analyses of such gene expression patterns: temporal models for gene expression patterns and comparative analysis of epigenomic contributions to gene expressions …
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Role of the cell cycle during human endothelial-to-haematopoietic transition
Haematopoietic stem cells (HSC) are responsible for the maintenance of blood homeostasis and are of fundamental importance for the treatment of a variety of life-threatening diseases. However, their scarce availability constitutes a major obstacle. Thus, understanding their embryonic specification …
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Modeling of gene regulatory networks controlling cell-fate decisions in Bacillus subtilis
To adapt to various environments, bacterial cells can activate distinct gene-expression programs and differentiate into mutually exclusive cell types. This process is called cell-fate decision. Bacillus subtilis is a well-studied model system for investigating bacterial cell-fate decisions. In …
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Prdm13, a Direct Downstream Target of Ptf1a, Regulates the Balance of Gabaergic Neurons versus Glutamatergic Neurons in the Dorsal Spinal Cord
… and is required for the glutamatergic neuronal fate. In contrast, another bHLH factor Ptf1a, is restricted in progenitors to dI4 and is required to specify the GABAergic neuronal fate. Since progenitors to dI4 express both Ascl1 and Ptf1a, some mechanism must exist to repress Ascl1 specification …
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Metabolic Regulation of Transcription Through Compartmentalized NAD+ Biosynthesis
Extracellular signaling and nutrient availability are major factors for the cell fate decision. Responds to extracellular information requires metabolic alterations and differential gene expression. Recently emerging concept of metabolic regulation of transcription reveals that fluctuation of …
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Mechanisms of Genome Buffering and Cell Fate Coordination in Adult Tissue Homeostasis
Self-renewal competency of adult stem cells is essential for tissue homeostasis. The corruption of genes essential for genome preservation or for niche-stem cell interactions frequently results in loss of stem cell viability and disease. The two components of my thesis focus on understanding adult …
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Interplay between interkinetic nuclear migration and cell fate determination in the developing zebrafish retina
… and directly imaged. All main retinal cell types are derived from multipotent retinal progenitor cells (RPCs), whose clonal sizes and compositions are stochastically determined. However, the biological processes that contribute to the stochastic cell fate decision making of RPCs remain …
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The role of space in homeostasis and preneoplasia in stratified squamous epithelia
… in biological research is the dynamics of stem cells in squamous epithelia. Given that most common human cancers develop from epithelia, understanding the rules of cell fate decision in these systems is key to explaining not only healthy tissue growth and maintenance but also the processes of …
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Pattern Formation During Extensive Cell Movements
… which impart spatial coordinates to individual cells. These coordinates then inform the cell of its respective fate, and hence a pattern of gene expression with spatial organisation is generated. This way of thinking about the problem of pattern formation has been highly successful in explaining …
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Role of multicellular organisation in mesoderm differentiation
… important for the differentiation of pluripotent cells in vitro and in vivo? During development, pluripotent cell fate decisions are spatiotemporally coordinated with changing tissue architecture. Typically, changes in tissue architecture are thought to be a downstream consequence of changes in …
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Studying early development using droplet single-cell RNA sequencing
… development is characterised by an increase in cell number and a diversification of the various cell types that are present. Development has been extensively studied not only to understand how cells commit to various lineages, but also with a view to reversing typical developmental pathways for …
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Mechanisms behind the fate of early chromosomal and transcriptional heterogeneities in the mouse embryo
… broad questions: 1) what mechanisms dictate the fate of the progenies of the chromosomally abnormal cells generated during the 4-8 cell stage division; 2) how the transcriptional heterogeneities between the blastomeres of the 4-cell stage embryo affect subsequent lineage segregation. A high …
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