Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 32 for “"Cell-based assay"”.
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Toward a Cell-Based Assay for Cubam-Mediated Intrinsic Factor-Vitamin B12 Binding and Uptake
… with the ultimate goal to establish a Ba/F3 cell model for evaluating IF-Cubam receptor interactions predictive of functionality in the human digestive tract. Methods: A Cubam/EpoR chimeric receptor was expressed on the cell surface, which was expected to activate the JAK-STAT5 pathway and …
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The identification of chemical compounds that decrease cellular levels of toxic Huntington's disease protein through a novel cell-based assay
… and cognitive symptoms. I sought to develop an assay system with the capability for identification of chemical compounds that selectively decrease the intracellular levels of disease-causing expanded polyglutamine huntingtin (Htt) protein without reducing the intracellular levels of the …
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Development and application of a high throughput cell based assay to identify apoptosis inducing proteins, and functional characterization of the candidate Vacuole Membrane Protein 1 (Vmp1)
… upon overexpression. To achieve this, a cell-based high throughput assay was developed. The assay is based on the detection of activated caspase-3 in cells overexpressing proteins tagged C- and N-terminally with YFP. Apoptotic cells were detected by staining with a specific antibody …
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Development and application of a high throughput cell based assay to identify novel modulators of ERK1/2 activation and,Functional characterisation of the candidate Radial spokehead like (Rshl1)
… human proteins that influence cancer relevant cellular processes like cell proliferation, signalling, and apoptosis upon over-expression. The focus of my work was 1) The establishment of a high throughput cell based assay to screen for proteins involved in the modulation of cell signalling …
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Design, Synthesis and Evaluation of Diphenylamines as MEK5 Inhibitors
… signal transduction kinases mediating intracellular responses to extracellular events. They are involved in mediating complex cellular events including, cell differentiation, cell proliferation, and cell death. Extracellular mitogens or the binding of other ligands to cell-surface receptors …
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Using photonic crystal biosensors for pharmaceutical screening
… and sensing is presented. A label-free biosensor assay is used to monitor the density of cell attachment to the sensor surface, and to quantify changes in attached cell density induced by the introduction of plant extracts. This image-based biosensor detection approach can effectively identify …
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Cell Targeted Ribosome Inactivating Proteins Derived from Protein Combinatorial Libraries
Combinatorial protein libraries based on a protein template offer a vast potential for deriving protein variants harboring new receptor specificity while retaining other tem-plate functions to serve as library search-engines, cell-routing sequences and therapeutic domains. This concept was tested …
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Small Molecule Inhibitors of HPV16 E6
… HPV 16 E6, one of two viral oncogenes, protects cells from apoptosis by binding to and accelerating the degradation of several apoptotic proteins, including caspase 8 and p53. We proposed that blocking the interactions between HPV E6 and its partners using small molecules had the potential to …
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Lipid sensitivity of the GPR132 receptor
… this family, GPR68, is activated by low pH extracellular conditions. However, GPR132 does not contain histidine residues at equivalent positions and is likely not to be activated by acid. GPR132 is described by the International Union of Basic and Clinical Pharmacology (IUPHAR) as an orphan GPCR, …
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Microfluidic devices for studying early response of cytokine signaling
… of a microfluidic device integrated with cell culture, cell stimulation, and protein analysis as a single device towards an efficient and productive cell-based assay development. In particular, this thesis demonstrates the feasibility of culturing human cancer cells in microliter-volume …
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Cellular and acellular assays for measuring oxidative stress induced by ambient and laboratory-generated aerosol
… species (ROS/RNS). This dissertation explores cellular and acellular measurements of PM-induced oxidative stress through systematic laboratory chamber experiments and ambient field studies. A cell-based assay involving murine alveolar macrophages was developed to measure intracellular ROS/RNS …
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Configurable and Up-Scalable Microfluidic Life Science Platform for Cell Based Assays by Gravity Driven Sequential Perfusion and Diffusion
… purification and real time nucleic acid sequence based amplification (NASBA) device. The device is manufactured using soft lithography and a unique silica bead immobilization method for the nucleic acid micro purification column. The integrated device produced a pathogen-specific response in < 3 …
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The Mechanisms of Tau Entry to the Cytosol
… of tau have been proposed to transit between cells of the brain in a ‘prion-like’ manner, resulting in templated aggregation of native tau in recipient neurons. Interactions between tau assemblies, surface receptor LRP1 and heparan sulphate proteoglycans promote the uptake of tau assemblies to …
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Identifying Genes in Pancreatic Beta-Cell Function using a Novel High-Thoughput Cell-Based Screening Assay
… insulin secretion from the pancreatic beta-cells. It is a global epidemic affecting millions of people around the world, bringing serious economic burden to health care systems. Thus, the underlying components of this disorder are under extensive investigation. The interest of my thesis …
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Photofrin-Mediated Electroporation-Assisted Photodynamic Therapy of MCF-7 Human Breast Cancer and B16-F10 Mouse Melanoma Cells
… creating reactive oxygen species that cause intracellular damage that ultimately brings about cell death. Photodynamic therapy (PDT) has the advantage over contemporary cancer treatment methods in that it is both non-invasive and can be tailored to largely avoid damage to noncancerous cells. The …
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Immunological and biochemical techniques in the analysis of tear proteins
… and unnecessary immune response. A novel on-lens cell based assay has been developed and exploited in order to study the role of the ubiquitous cell adhesion glycoprotein, vitronectin, in tears and contact lens wear under various parameters. Vitronectin, whose levels are known to increase in the …
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Characterisation of the structural motifs Involved in the cleavage and secretion of human angiotensin-converting enzyme
… cleavage of membrane proteins is an essential cellular process that controls their expression and function, and modulates cellular and physiological processes. Testis ACE (tACE) is shed at a higher rate than somatic ACE and it has been proposed that regions in its ectodomain direct its …
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Use of Echogenic Immunoliposomes For Delivery of Both Drug and Stem Cells For Inhibition of Atheroma Progression
… for Delivery of both Drug and Stem Cells for Inhibition of Atheroma Progression </strong></p> <p>By Ali K. Naji B.S. Advisor: Dr. Melvin E. Klegerman PhD</p> <p><strong>Background</strong> and significance: Echogenic liposomes can be used as drug and cell delivery vehicles that …
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TRPML1: Role In Autophagy And Potential Target To Treat Lysosomal Storage Disorders
The view of the lysosome as the terminal end of cellular catabolic pathways has been challenged by recent studies showing a central role of this organelle in the control of cell function. Here we show that a lysosomal Ca2+ signaling mechanism controls the activities of the phosphatase calcineurin …
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