Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 53 for “"Cell-Substrate"”.
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Effect of Cell-Substrate Interactions on Epithelial Cell Mechanics
Cell-substrate interactions play a key role in the regulation of epithelial cell mechanics. Through a series of studies, we demonstrate how substrate interactions impact both the response to an oncogene and the cellular contractility and organization of a monolayer. We first examine the effects of …
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Biomolecularly and mechanically instructive materials for guiding cell-substrate interactions
… advance our understanding and ability to guide cell fate and function. Benzophenone (BP) photolithography is uniquely suited to generating chemically and mechanically tailored materials because of its molecularly general and spatially modulated characteristics. Here, we describe the application …
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COMBINATORIAL DEVELOPMENT OF NANOSTRUCTURED MATERIAL LIBRARIES FOR THE STUDY OF CELL SUBSTRATE INTERACTION
An in vivo cellular microenvironment in which cells are immobilized contribute an essential role in diverse cellular behavior, consisting of cellular morphology, dynamics and eventually cellular fate. Micro and nanofabrication tools widely used to mimic the in vivo surrounding to manipulate …
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In vitro models for airway epithelial cell culture
… of the human airway. Various factors such as the cell model, physiochemical factors such as the cell substrate properties including its stiffness, shear stress, stretch, the air-liquid interface and the biochemical factors in the medium influence the biology of the cells. The aim of this work is …
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Design of Biomembrane-Mimicking Substrates of Tunable Viscosity to Regulate Cellular Mechanoresponse
Tissue cells display mechanosensitivity in their ability to discern and respond to changes in the viscoelastic properties of their surroundings. By anchoring and pulling, cells are capable of translating mechanical stimuli into a biological response through a process known as mechanotransduction, a …
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The effects of mechanical forces on lymphocyte adhesion and migration
… is depressed under microgravity conditions. Cells may sense altered gravitational forces through changes in the mechanical forces they experience. To study the effects of microgravity on lymphocytes using ground-based methods, we examined the effects of mechanical forces on cellular function. …
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Probing cellular mechano-sensitivity using biomembrane-mimicking cell substrates of adjustable stiffness
… recognized that mechanical properties of substrates play a pivotal role in the regulation of cellular fate and function. However, the underlying mechanisms of cellular mechanosensing still remain a topic of open debate. Traditionally, advancements in this field have been made using …
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First evidence of skelemin, a myosin-associated protein, in smooth muscle and its involvement in cell adhesion, and, The role of the cell cycle in cell type choice during mammalian development
… underexpression was seen to cause loss of cell-cell as well as cell-substrate adhesion. Time lapse video microscopy was used to analyze the role of the cell cycle in cell type choice during mammalian development. Discussed are methods employed to improve resolution for accurate analysis.
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Investigations into endothelial cell permeability and adherens junction disruption induced by Junín virus infection.
… to produce productive infection of endothelial cells in vitro with no visible cytopathic effects which provides a unique opportunity to study the cells while they are infected. Here, we show that direct Junín virus infection of primary human endothelial cells (EC) corresponds to increased …
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Identification of Cell Biomechanical Signatures Using Three Dimensional Isotropic Microstructures
… biology (BioMEMS) enables precise control of the cellular microenvironments and offers high throughput systems. The focus of this research was to develop three dimensional (3-D) isotropic microstructures for comprehensive analysis on cell-substrate interactions. The aim was to investigate whether …
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Nanoscale and microscale approaches for engineering the in vitro cellular microenvironment
… and microfabrication approaches to manipulate cell microenvironment in culture. To control cell migration and to restrict cell or colony size, cells and proteins were patterned by using molding or printing methods. Poly(ethylene glycol)-based molecules and polysaccharides were used to control …
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Development of poly(3-octylthiophene) thin films for regulating osteoblast growth
… applications and establishes P3OT as a potential cell substrate that sustains MC3T3-E1 attachment and promotes high levels of cell proliferation.
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Design and control of a cellular architecture-based adaptive wiring manifold
… in this thesis that demonstrates the concepts of substrate/cell creation, master routing control and graph creation, and wiring commands generated and transmitted to the cell substrate in order to route electrical connections based on gathered netlists of detected components.
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PROBING CELLULAR MECHANO-SENSITIVITY USING BIOMEMBRANE-MIMICKING CELL SUBSTRATES OF ADJUSTABLE STIFFNESS
… recognized that mechanical properties of substrates play a pivotal role in the regulation of cellular fate and function. However, the underlying mechanisms of cellular mechanosensing still remain a topic of open debate. Traditionally, advancements in this field have been made using …
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Molecular fluorescent reporters for force and smart surfaces for sensing cell-surface interaction
… relationship, and adapted the sensor to a cell adhesion assay. This thesis focuses on the considerations for combining force measurement with the environmental and distance sensitivity offered by fluorescence to measure cell-surface adhesion. We showed that DNA can be used as a scaffold to …
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Development of a direct photochemical method for the generation of biomolecular interfaces for applications in leukocyte biology
… photochemical method for creating biointerface substrates presenting single- and multi-component biomolecular patterns and gradients. In our approach, the generation of a light density gradient across a photoactive benzophenone monolayer on glass results in covalent attachment of solution-phase …
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Investigating spatial distribution and dynamics of membrane proteins in polymer-tethered lipid bilayer systems using single molecule-sensitive imaging techniques
… in the areas of membrane biophysics and cellular mechanics. From the membrane biophysics perspective, the role of lipid heterogeneities in the distribution and function of membrane proteins in the plasma membrane has been a long-standing problem. One of the most well-known membrane …
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The Cx43 Carboxyl-Terminal Mimetic Peptide αCT1 Protects Endothelial Barrier Function in a ZO1 Binding-Competent Manner
… barrier function measurement, including Electric Cell-Substrate Impedance Sensing(ECIS), a TRITC-dextran transwell permeability assay, and a FITC-dextran cardiovascular leakage protocol involving Langendorff-perfused mouse hearts, αCT1 was found to protect the endothelium from thrombin-induced …
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Cell attachment to Zein surfaces
… as tissue engineering and circulating cancer cell retrieval. Understanding the mechanism of cell-substrate interaction is pivotal for exploiting the potential of those materials. Adhesion of cells to substrate surfaces is often mediated by tissue transglutaminase (tTG) in its role as a …
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