Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 39 for “"Cell-Seeding"”.
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Characterization and Cell-Seeding of Decellularized Adipose Tissue Foams for Wound Healing
… in healing these wounds so alternative cell-based approaches are being investigated. Each cell-based approach necessitates a delivery strategy that minimizes cell death and maximizes cell retention. To this end, an adipose-derived stem cell (ASC) delivery system was developed, using an …
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Cell seeding and proliferation simulations to plan experiments of multicellular osteogenic systems
Osteogenesis in vivo involves changes to cell density, morphology, cell-cell contacts, gene expression, and proliferation; however, the coordination of these changes is unknown. Specifically, cells undergo a morphological switch; they are proliferative, pluripotent, elongated cells early in …
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Design and development of a bioreactor for ligament tissue engineering
Mechanical signals applied in-vitro to cell-scaffold construct may induce the formation of ligament-like structure having in-vivo functional properties. To explore this hypothesis, a novel bioreactor was designed to study the in vitro effect of biaxial cyclic mechanical stimulation on the adhesion, …
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Microfluidic device for cytotoxicity screening
… be detrimental to the proliferation of mammalian cells. Current cytotoxicity screening method requires initial cell seeding in 96-well cell culture plates and a large volume of culturing media, overall a time-consuming and resource-intensive process. We have developed a microfluidic device with …
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The mechanics of cell-seeded collagen hydrogels
… namely permeability and stiffness, of HepG2 cell-seeded collagen hydrogels. Confined compression testing in conjunction with biphasic theory modelling was utilised to provide measures of these mechanical parameters of interest. Different cell seeding densities and culture durations were …
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Biodegradable polymer scaffold fabrication and the creation of tissue-engineered bone
… has been proposed which utilizes autologous cell transplantation within porous biodegradable polymer foam scaffolds. The work presented in this thesis was aimed at creating a porous biodegradable scaffold with appropriate mechanical properties, which in concert with an appropriate vascular …
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Tissue Engineering the TMJ Condyle using Human Umbilical Cord Mesenchymal Stromal Cells
… thesis, human umbilical cord mesenchymal stromal cells (hUCMSCs), identified as multipotent cells only in this decade, had not yet been used for TMJ tissue engineering. Hence, this thesis proposed a revolutionary tissue engineering approach in which hUCMSCs and mature hyaline cartilage cells, …
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Cardiac tissue engineering : bioreactor cultivation parameters
… system consisting of isolated cardiac cells, polymer scaffolds, and tissue culture bioreactors, we could engineer cardiac muscle mimicking native tissue in structure and function in the presence of appropriate biochemical and physical signals. The specific objectives were to: ( 1) vary …
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Modeling cell proliferation in a perfusion tissue engineering bioreactor
… takes place in a bioreactor in which a cell seeded porous scaffold is placed, and the scaffold experiences a perfused flow of a nutrient-rich culture medium. The goal of the model is to assist experimentalists in evaluation of different parameter scenarios as the time needed to simulate …
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Development of a Cell Depositing System Using Inkjet Technology
… first be fabricated then exposed to a sea of cells in the hopes of seeding. Furthermore, even though cells may have attached, more time must be spent in order to allow the cells to migrate to their ideal locations. To deal with this problem, researchers have investigated whether rapid …
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Vascular Network Formation via 3D Printing and Cell-Based Approaches
… bioprinting, and sacrificial templating; and cell-based approaches, in which cellular self-organization processes form vessels; all are deficient in their ability to form a vessel system of sufficient complexity for supporting a large cellular construct. What is missing from the literature is …
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The Role of Biomechanics in Liver Tissue Engineering
… tissue engineering is to use the process of decellularization to create acellular liver scaffolds that retain the native tissue's extracellular matrix and architecture. These scaffolds can then be repopulated with a variety of hepatic and non-hepatic cell-types depending on the application, or …
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Inhibiting metastatic potential: an analysis of migratory body formation in chondrosarcoma
… might occur. Our in vitro observations with CHS cell-line SW1353 have shown that spheroids which we call migratory bodies (MB) can detach from the monolayer in our cell culture plates and remain suspended in the media. These MBs have shown to be able to adhere and proliferate, forming new …
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Proliferation and morphology of pre-osteoblast cells on 25 – 1000 micron diameter patterned features on polyacrylamide hydrogels
… distinct phases: proliferation, during which cells multiply, form nodules, and begin expressing early stage osteogenic markers; extracellular matrix development and maturation, during which mature cells in nodules build a collagen matrix and secrete the building blocks for mineralization; and …
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Finding Common Ground to Treat Primary and Metastatic Cancer: The Potential of Targeting Tumor Stroma
… stroma formed by accumulation of hematopoietic cells, mesenchymal stromal cells and extracellular matrix. The crosstalk between malignant epithelial cells and tumor stroma is becoming increasingly appreciated as a key determinant in tumor development, progression and metastasis, as well as …
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Investigating Cell Viscoelastic Properties with Nanonet Force Microscopy
Determining the mechanical properties of living cells accurately and repeatably is critical to understanding developmental, disease, and repair biology. The cellular environment is composed of fibrous proteins of a mix of diameters organized in random and aligned configurations. In the past two …
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Development Of Scaffold Architectures And Heterotypic Cell Systems For Hepatocyte Transplantation
… of a useful size, while maintaining full cell viability, an issue which is closely related to the high metabolic rate of hepatocytes. In this study, we first compared two designs of highly porous chitosan-heparin scaffolds seeded with hepatocytes in dynamic perfusion bioreactor systems. …
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Modulation of cell morphology and function using synthetic biodegradable polymers
… into thin films for use as scaffolds for cell transplantation and guided tissue regeneration. We evaluated the ability of PLGA films to provide a suitable template for directing specific cell growth and tissue formation using cells derived from retinal pigment epithelium (RPE) as an in …
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Enhancing Mesenchymal Stem Cell Chondrogenesis for Cartilage Tissue Engineering
… replacement tissues, and mesenchymal stem cells (MSCs), which can undergo chondrogenesis, have emerged as a promising cell source. To date however, the functional properties of MSC-based constructs remain lower than those of the native tissue and of chondrocyte-based constructs cultured …
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Engineering Biodegradable Vascular Scaffolds for Congenital Heart Disease
… The bioreactor applied fluid flow to support cell seeding, differentiation, and growth of endothelial progenitor cells on the grafts, demonstrating a robust strategy for tissue formation prior to implantation. The second component of this research centers on the development of a biomaterial …
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