Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 45 for “"Cell wall biosynthesis"”.
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Evaluating methiozolin programs for golf putting greens and investigating potential modes of action
… but has been proposed to act as either a cell wall biosynthesis inhibitor (CBI) or an inhibitor of tyrosine aminotransferase (TAT). Field studies were conducted at Virginia Tech to investigate strategies promoting surface recovery on putting greens following atypically rapid annual …
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Mechanisms of bacterial polyprenyl transferases and multi-target drug discovery for tuberculosis
… entities that are involved in bacterial cell wall biosynthesis, biofilm formation, virulence formation and natural product formation. Structurally, bacterial polyprenyl transferases can be categorized into eight groups: αcyclase, α, αβ, αβγ, βγ, ε, ζ and TIM barrel fold proteins. Six …
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Multi-target drug discovery against Staphylococcus aureus and Trypanosoma brucei infections
… synthase (UPPS), an essential enzyme involved in cell wall biosynthesis pathway. Besides, they bound to an AT-rich DNA dodecamer (CGCGAATTCGCG)2 and were found to increase the melting transition by up to 24 °C using differential scanning calorimetry (DSC). Good correlations (R2 = 0.89, S. aureus) …
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Elucidating Targets for Infectious Diseases and Cancer: A Computational Approach
… relating to parasitic diseases, bacterial cell wall biosynthesis, and cancer. These modeling efforts have not only resulted in more potent inhibitors, but have also increased our understanding of these disease targets, which include T. cruzi hexokinase, farnesyl diphosphate synthase, …
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The synthesis of aminophosphonic acid derivatives & related compounds
… as antibacterial agents, which inhibit bacterial cell wall biosynthesis; as structural units in peptidic phosphonylating agents that irreversibly inhibit a wide variety of serine proteases; as structural units in butyloxycarbonyl protected phosphonate esters that complex with alpha-lytic …
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Targeting isoprenoid and quinone biosynthesis for antimicrobial discovery
"Enzymes in isoprenoid biosynthesis pathway play important roles in all living organisms. Here we report the first structure of heptaprenyl diphosphate synthase from Staphylococcus aureus (SaHepPPS) together with an investigation of its mechanism of action, and inhibition. HepPPS is involved in …
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Design and synthesis of inhibitors of dTDP-D-glucose 4,6-dehydrate (Rm1B), and enzyme required for dTDP-L-rhamnose production in M. tuberculosis
… developed to investigate a pathway involved in cell wall biosynthesis as a promising target: the dTDP-L-rhamnose pathway. This pathway plays an essential role in linking the peptidoglycan and arabinogalactan portions of the mycolic acid-arabinogalactan-peptidoglycan complex, a significant part …
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Investigation into peptidoglycan biosynthetic enzymes from the Lyme disease spirochete Borrelia burgdorferi
… dissertation explores the enzymes involved in PG biosynthesis in B. burgdorferi, focusing on Mur ligases, which are key to constructing the peptide stem of PG. An in vitro assay was developed to characterize MurE activity, leading to the discovery that BB0201 encodes a functional MurE ligase that …
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Characterization of D-boroala as a novel Broad-spectrum antibacterial agent targeting D-Ala-D-Ala ligase & designer linkers : model reactions and linear free energy relationships in the reactivity and design of solid-phase linkers
… transition-state analog inhibitors for bacterial cell wall–synthesizing enzymes, we observed that D-boroAlanine (D-Ala with the -COOH group replaced with a -B(OH)2 group) had effective antibacterial activity. In the first part of this dissertation, we describe the antibacterial properties of …
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Targeting isoprenoid biosynthesis for drug discovery
… there is a need for new drugs, and isoprenoid biosynthesis is an attractive target for drug discovery. In chapter 1, I briefly introduce the isoprenoid biosynthesis pathway and two important drug targets: a trans-prenyl transferase farnesyl diphosphate synthase (FPPS), and a cis-prenyl …
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Design, Synthesis, and Evaluation of Small Molecules in the Discovery of Novel Antimicrobial Agents
… inhibitors of Mycobacterium tuberculosis cell wall biosynthesis via the rhamnose pathway. Chapter 3 describes the discovery of novel inhibitors of the PlsX/PlsY pathway to phosphatidic acid, a key intermediate in the biosynthesis of phospholipids in Gram-positive bacteria. Substrate …
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Cellulose biosynthesis inhibitors modulate defense transcripts and regulate genes that are implicated in cell wall re-structuring in arabidopsis
The cell wall is a multifunctional structure which is implicated in plant growth and development as well as responding to any environmental changes including biotic and abiotic stresses. One of the practical approaches in cell wall integrity studies is the modification of the quality and quantity …
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Defining the tropism of Listeria monocytogenes using a comparative genomics approach
… it was found that this enzyme is involved in cell wall biosynthesis and could be responsible for increased survival of lineage II strains in the host environment, thus rendering these isolates more prevalent in cases of human listeriosis.
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Elucidation and Characterization of Glycosyltransferases Implicated in Pectic Polysaccharide Biosynthesis in Arabidopsis thaliana
The cell wall is a complex extracellular matrix composed of cellulose, hemicellulose, and pectic polysaccharides that confers multiple functions to plant cells and the plant as an organism. Glycosyltransferases (GTs) are enzymes that catalyze the transfer of a sugar molecule from an activated …
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Biosynthetic and Chemical Investigation of Lipid II-Binding Antimicrobials.
… act as potent antimicrobial agents by disrupting cell wall biosynthesis via sequestering the late-stage intermediate lipid II. However, the emergence of resistance mechanisms and poor bioavailability have hindered the utility of these molecules as promising therapeutic intervention strategies to …
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Two strategies to deepen knowledge the antibiotic resistance related traits in Italian MDR Staphylococci: comparative transcriptomic of two DAP-R/DAP-S MRSA isogenic couples and CRISPR system characterization of pathogenic S. epidermidis
… with multiple pathways associated, including the cell-wall biosynthesis and organization, metabolism, nucleic acid metabolism, stress response and transport, confirming the role of transcriptome in developing antibiotic resistance. CRISPR-Cas system presence seems to not be a common trait in MDR …
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Structural And Functional Characterization Of Proteins Of Unknown Function (hp0495, Hp0100 And Hp1259) In Helicobacter Pylori
… mraY and murD, these two enzymes are involved in cell wall biosynthesis, suggesting that Hp0495 might be involved in regulating this process. There is also precedence for the involvement of EF-Tu in cell wall biosynthesis, supporting this hypothesis. </p> <p>In collaboration with a colleague, Dr. …
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Studies of unusual catalysis : a tale of four enzymes from diverse biosynthesis pathways
… for decades. Continuing investigations in the biosynthesis of both primary and secondary metabolites have led to the discovery of enzymes that employ unusual ways to mediate bio-transformations. Exploration of such atypical biological catalysts is not only important for a comprehensive …
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Assays for lycosyltransferases
… a large enzyme family that are involved in the biosynthesis of complex carbohydrates and glycoconjugates. These glycosylation reactions are essential to many fundamental biological processes, including cellular adhesion, cell signalling and bacterial cell wall biosynthesis. The aim of this …
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