Global ETD Search
Search theses and dissertations gathered from participating repositories worldwide. Every result links back to the library that holds it. No account is needed.
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Showing 1 to 20 of 47 for “"Cell transformation"”.
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Effects of conjugated oligoelectrolytes on cell transformation
… is the delivery of genetic material into the cell. Increasing bacterial cell transformation efficiency can help advance an area of scientific research with a wide range of applications. Previous work has demonstrated conjugated oligoelectrolytes (COEs) neutralize the surface charge of …
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Investigating mitotic vulnerabilities that arise upon oncogenic cell transformation
During mitosis, cells must accurately divide their duplicated chromosomes into two new daughter cells. This process is highly regulated and much of this regulation is centered around kinetochores. Kinetochores are large proteinaceous structures built upon centromeric heterochromatin that must form …
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Identification and Characterization of Genes Associated with <i>V-Jun</i> Induced Cell Transformation
… an unregulated transcription factor would cause cell transfonnation by illicit regulation of its target genes. In support of this, we show that <em>in vivo</em> v-Jun complexes exhibit differential binding to <em>in vitro</em> generated AP-1 and 'AP-1 like' target sequences, suggesting that the …
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Characterization of the effects of decreased expression of ribosomal proteins on cell transformation and cell cycle regulation
Thesis (Sc. D.)--Massachusetts Institute of Technology, Division of Toxicology, 1996.
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Investigating the role of the Epstein-Barr virus-encoded oncoprotein, latent membrane protein 1, in cell transformation in vitro
… and is known for its ability to transform B cells, epithelial cells and rodent fibroblasts in vitro. When expressed in Madin-Darby Canine Kidney (MDCK) epithelial cells, LMP1 induces an epithelial-mesenchymal-transition (EMT), assuming a mesenchymal phenotype. The tumour microenvironment …
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A Role for Oligomerization in the Multifunctional Nature of the Simian Virus 40 Large T Antigen Protein (Tumor, Dna, Cell Transformation, Association)
… of the virus in permissive hosts and in the transformation of non-permissive hosts to a tumorigenic phenotype. Biochemical studies reveal that large T antigen has several activities detectable in vitro. While the multifunctional nature of this protein is already generally accepted, the …
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Biology of the envelope glycoproteins of sheep betaretroviruses
… associate, modulate and alter the biology of the cell with a variety of unique mechanisms. Integration of retroviruses into the germ line of the host results in the formation of vertically transmitted “endogenous” retroviruses (ERVs). It is now becoming apparent that ERVs have often been selected …
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Translational Regulation of the C-Jun Proto-Oncogene
… to understand the role of the v-jun oncogene in cell transformation. The c-Jun protein is a transcription factor which binds to the DNA target TGACTCA. The c-Jun protein binds to DNA in the form of dimers. It can form homodimers with itself and heterodimers with Jun family (JunB and JunD), Fos …
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Of Mice and Men: Studying Innate and Adaptive Immunity Against the Epstein-Barr Virus
… This transforming property can be observed as B cell transformation in vitro and lymphomas as well as epithelial cancers in vivo, but most immunocompetent individuals control EBV infection successfully without the occurrence of disease. Cells of the innate immune system act in synergy to provide …
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Expression of Follicular Helper T-cell Markers in Primary Cutaneous T-cell Lymphoma
… and objectives: A distinct subset of T helper cells, named follicular helper T- (TFH) cells, have been recently described. The TFH-cells can be identified on the basis of the expression of several markers including: the chemokine CXCL13; the costimulatory molecules PD-1 and inducible …
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Optimizing Conditions for Cloning cDNA from Chicken Brain
Acoustic over stimulation can lead to hair cell loss in the auditory epithelium. Hair cell loss or damage in mammals often results in decreased auditory sensitivity which is irreversible. However for birds, hair cells which are damaged by acoustic trauma or ototoxic drugs may be replaced by …
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HPV16 E6* Induces Oxidative Stress and DNA Damage
… E6 and E7, which work together to initiate cell transformation. The approximately 50 amino acid product of the E6* transcript is expressed during the early stages of HPV infection. In this study, we found that expression of E6* increased the level of reactive oxygen species (ROS) in both …
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Harnessing synergy between BCL6 and PI3Kδ to generate a new mouse model for lymphoma
… 3-kinase (PI3K) pathway is critical for immune cell development and function. Patients with a gain of function mutation in PI3Kδ, called Activated PI3Kδ syndrome (APDS) commonly develop autoimmunity, lymphoproliferation, and lymphoma. PIK3CD is also among the most commonly mutated genes in …
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14-3-3Sigma Negatively Regulates The Stability and Subcellular Localization of Cop1
… We discovered that 14-3-3σ controlled COP1 subcellular localization and protein stability. Investigation of the underlying mechanism suggested that, upon DNA damage, 14-3-3σ bound to phosphorylated COP1 at S387, resulting in COP1 translocation to the cytoplasm and cytoplasmic COP1 …
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Detecting Changes in Alternative mRNA Processing From Microarray Expression Data
… changes in global mRNA processing during B cell transformation and show that the conventional U133 platform is able to detect 3' located changes in mRNA processing five times more frequently than the Human Exon array.</p>
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Control of the EBV growth transformation programme: the importance of the Bamhi W repeats
… latent genes whose constitutive expression in B cells leads to cell growth transformation. The initiation of this B-cell growth transformation programme depends on the activation of a viral promoter, Wp, present in each tandemly arrayed BamHI W repeat of the EBV genome. In order to examine the …
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Functional characterisation of the N-terminal domain of polyomavirus large T antigen
… the relationship between viral oncoproteins and cellular tumour suppressor proteins in recent years, the molecular interactions between these proteins is still poorly understood. It is the goal of this thesis to establish the key elements of specific interactions, in particular to characterise …
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Identifying the Molecular Mechanism of TAZ-Induced Lung Tumorigenesis
… plays critical roles in organ size control, stem cell self-renewal, tumorigenesis and drug resistance. Recently, TAZ has been identified as a novel oncogene that is overexpressed in non-small cell lung cancer (NSCLC) cells and mediates their proliferation, transformation and tumorigenesis. …
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Investigating the role of mRNA capping enzyme in C-MYC function
… pol II-associated CE activity. Interestingly, cells driven by C-MYC are highly dependent on CE for C-MYC-induced target gene expression and cell transformation, but only when C-MYC is overexpressed; C-MYC-independent cells or cells retaining normal control of C-MYC expression are insensitive to …
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Identification and functional characterisation of RAM, a novel and essential component of RNA guanine-7 methylation
… methylation is an essential process for cell viability and its up-regulation has been associated with cell transformation. However, the mechanistic details of RNMT function in mammalian cells remain elusive. In order to gain better understanding of the molecular mechanisms associated with …
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